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Method for extracting and preparing total flavonoids of Hibiscus mutabilis

A technology of hibiscus leaves and total flavonoids, which is applied in the field of extraction and preparation of hibiscus leaves total flavonoids, can solve the problems of large loss of extracted components, residual organic solvents, and low efficiency, and achieve high extraction efficiency, mild reaction conditions, and simple operation.

Inactive Publication Date: 2019-12-31
湖州耕香生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the common shortcomings of traditional extraction methods such as low efficiency, residual organic solvents, and large loss of extracted components, it is urgent to study the application of some new solvent extraction methods in the total flavonoids of hibiscus leaves

Method used

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  • Method for extracting and preparing total flavonoids of Hibiscus mutabilis
  • Method for extracting and preparing total flavonoids of Hibiscus mutabilis
  • Method for extracting and preparing total flavonoids of Hibiscus mutabilis

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Experimental program
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Effect test

Embodiment 1

[0021] The preparation method of embodiment one deep eutectic solvent

[0022] All chemical reagents, including choline chloride, urea, ethylene glycol, glycerol, 1,3-butanediol, citric acid, glucose, oxalic acid, acetamide, by hydrogen bond acceptor: hydrogen bond donor = Fixed molar ratio of 1:2 Weigh the chemical reagents and mix them in a beaker. Place the beaker in a magnetic stirrer and heat it in a water bath at a temperature of 80°C. Stir it magnetically for about 30 minutes to obtain a uniform and transparent liquid. Cooled, no crystallization or other abnormal phenomena.

Embodiment 2

[0023] The color development method and the absorbance measurement of embodiment diflavone

[0024] Precisely draw 1ml of sample solution into a 25ml volumetric flask, add 5% NaNO 2 Solution 1ml, shake well, stand for 6min; add 10% Al(NO 3 ) 3 1ml of the solution, shake well, stand for 6min; add 10ml of 4% NaOH solution, add to the mark with pure water; shake well, stand for 15min, then measure the UV absorbance at 510nm.

[0025] Establishment of the standard curve: Dilute the rutin standard substance (purchased from Shanghai Yuanye Biotechnology Co., Ltd., model B20771, HPLC≧98%) with 65% ethanol aqueous solution to a concentration of 0.021mg / mL and 0.042mg respectively. / mL, 0.063mg / mL, 0.084mg / mL, 0.105mg / mL control solution, NaNO 2 -Al(NO 3 ) 3 -After NaOH color development, measure the UV absorbance at 510nm, and obtain absorbance values: 0.180, 0.361, 0.551, 0.719, 0.905. Taking the rutin concentration as the abscissa and the absorbance as the ordinate, draw the s...

Embodiment 3

[0026] The investigation of embodiment three deep eutectic solvent composition types

[0027] In this embodiment, hibiscus leaves are used as flavonoid raw materials

[0028] A method for extracting and preparing total flavonoids from hibiscus leaves, comprising the following steps:

[0029] (1) hibiscus leaf raw material is dried in the vacuum blast drying box of 50 ℃, pulverizes, passes No. 4 sieves that " Pharmacopoeia of the People's Republic of China " stipulates;

[0030](2) Accurately take 8 parts of Hibiscus leaf powder 2.000g after sieving, according to the ratio of material to liquid 1:10, the deep eutectic solvent water content is 40%, choline chloride: hydrogen bond donor=1:2 conditions, hibiscus leaf powder was mixed evenly with the prepared 8 kinds of deep eutectic solvents, and extracted with ultrasonic assistance, the extraction temperature was 40 ° C, and the extraction time was 30 minutes; after the reaction was completed, 8 kinds of deep eutectic solvents w...

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Abstract

The invention provides a method for extracting and preparing total flavonoids of Hibiscus mutabilis. The method comprises the following steps: (1) drying, crushing and sieving a Hibiscus mutabilis leaf raw material; and (2) uniformly mixing the sieved Hibiscus mutabilis leaf powder with an eutectic solvent, carrying out ultrasonic-assisted extraction and performing suction filtration to obtain a Hibiscus mutabilis leaf extracting solution containing total flavonoids, and carrying out rotary evaporation and concentration to obtain the total flavonoids of the Hibiscus mutabilis leaves. Preferably, a sieve in the step (1) is a No.4 sieve specified in Pharmacopoeia of People's Republic of China. Preferably, in the step (2), the screened Hibiscus mutabilis leaf raw material and the eutectic solvent are mixed according to the weight-volume ratio of 1: 5-1: 35 in terms of the solid-liquid ratio.

Description

technical field [0001] The invention belongs to the field of medicinal material extraction technology, in particular to a method for extracting and preparing total flavonoids from Hibiscus hibiscus leaves. Background technique [0002] Hibiscus leaf is a traditional Chinese medicine in my country, also known as hibiscus leaf and anti-shuang leaf. It has a long history and is recorded in many ancient medical books. "Bencao Tujing" and "Compendium of Materia Medica" record its characteristics as "pungent, flat, non-toxic", and has pharmacological effects such as cooling blood and detoxification, reducing swelling and relieving pain. Modern pharmacological experiments have proved that the effective extracts from hibiscus leaves can inhibit insects and bacteria in vitro, resist non-specific inflammation, and protect renal ischemia-reperfusion injury. Among them, flavonoid glycosides are one of its main active ingredients, mainly including rutin, kaempferol-3-O-β-rutinoside, kae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K127/00
CPCA61K36/185A61K2236/333
Inventor 王平蒋锦燕江瑜
Owner 湖州耕香生物科技有限公司
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