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Method for inducing callus of clematis 'Yongshiwu'

A technology of callus induction and callus, applied in the field of plant propagation, can solve the problems of lagging development speed, low rooting rate of cutting propagation, affecting the industrialization development of excellent clematis varieties, etc., to increase the number of induction, split powerful effect

Inactive Publication Date: 2020-01-03
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The seed reproduction rate of clematis is very low, and the rooting rate of cutting propagation is not high. The use of tissue culture and somatic embryogenesis is a very promising approach, and large-scale production can be obtained by using tissue culture. However, the tissue culture of clematis is domestically There are few studies, and the development speed is relatively lagging behind, which seriously affects the industrial development of excellent clematis varieties

Method used

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  • Method for inducing callus of clematis 'Yongshiwu'
  • Method for inducing callus of clematis 'Yongshiwu'

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Embodiment 1

[0021] The method for inducing callus of Clematis "Warrior Dance" ('Krakowiak') of the present invention specifically comprises the following steps:

[0022] (1) Obtain sterile materials:

[0023] Cut the semi-lignified branches of clematis in winter, and the cut branches are stem sections with buds, which are disinfected and sterilized; the disinfection and sterilization treatment is specifically: first use 0.1 Soak in % washing powder for 8-10 minutes, then rinse with tap water for 60 minutes; after washing, first soak with 0.1-0.2% chlorothalonil for 5-10 minutes, after soaking, rinse with sterile water for 3 times, then soak with carbendazim for 5-10 minutes, Then rinse it with running water for 3 times, and finally put it on the ultra-clean workbench, soak it with ethanol with a mass fraction of 75% for 30-60 seconds, rinse it with sterile water for 3 times after soaking, and then add 1 drop of Tween 80 Soak in mercuric chloride solution with a fraction of 0.1% for 6-8 m...

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Abstract

The invention discloses a method for inducing callus of clematis 'Yongshiwu'. The method comprises the following steps: (1) obtaining of a sterile material: cutting a semi-lignified stem segment withbuds of the clematis in winter, and sterilizing the stem segment with buds; (2) primary culture: cutting the sterilized branches into 1-2 cm stem segments, carrying at least one healthy full bud on each stem segment, inoculating the stem segments on an initial culture medium, germinating the buds on the branches seven days after the primary culture is carried out, and transferring the buds into acallus induction culture medium for callus induction culture; (3) callus culture: when the callus appears in the callus induction culture medium, inducing cluster buds on the callus after 20-25 days of culture, cutting the cluster buds into single plants or small stem segments, inoculating the single plants or small stem segments again into a new callus induction culture with the pH value of 5.8-6.2, greatly differentiating the cluster buds from the callus after 20-25 days of culture, and keeping culturing to induce adventitious roots.

Description

technical field [0001] The invention relates to a method for inducing callus of clematis "Warrior Dance", which belongs to the technical field of plant propagation. Background technique [0002] Clematis belongs to the genus Clematis sp. of Ranunculaceae (Ranunculaceae), which is a kind of vines with high ornamental value and various stress resistance. Clematis are perennial woody or herbaceous vines, and a few are erect shrubs. It has a long flowering period, strong adaptability, and strong cold resistance, and occupies an important position in international ornamental horticulture. Today, in the pursuit of new, strange and special flower varieties, the ornamental value of clematis plants has been paid more and more attention by the horticultural circles at home and abroad. The seed reproduction rate of clematis is very low, and the rooting rate of cutting propagation is not high. The use of tissue culture and somatic embryogenesis is a very promising approach, and large-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 尹富闯宋微王磊张虎
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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