Composition for increasing hair quantity in follicular unit and preparation method thereof
A composition and unit technology, applied in the directions of drug combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve problems such as the small number of hairs, and achieve the effects of promoting hair follicle health, improving oil environment, and promoting sustainable healthy growth.
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specific Embodiment approach 1
[0041]Embodiment 1: The composition for increasing the number of hairs in the follicular unit of this embodiment includes liquid A, liquid B, and liquid C; the volume ratio of liquid A, liquid B, and liquid C is (1-2):(0.5-1) :(7-8.5);
[0042] Liquid A is hair follicle telogen inhibitor, including lactic acid 0.1-10.0mg / ml, citric acid 0.1-1.5mg / ml, gluconic acid 1.0-4.4mg / ml, tryptophan 3-30μg / ml and deionized water ;
[0043] Liquid B is an inflammatory control agent, including interleukin-1 receptor antagonist IL-1ra 3.0-14.0ng / ml, soluble tumor necrosis factor receptor-1 10.0-30.0ng / ml and deionized water;
[0044] Liquid C is a hair growth promoter, that is, super active platelet lysate (sPL).
specific Embodiment approach 2
[0045] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the preparation method of the C liquid is:
[0046] A. Collect autologous blood, add anticoagulant to obtain anticoagulant blood, centrifuge at 20°C, 200g-250g for 10 minutes, remove the lower red blood cells; then mix the remaining blood in the centrifuge tube evenly, and then centrifuge at 22°C, 3000g After 15 minutes, the upper 4 / 5 plasma was removed, and the bottom 1 / 5 plasma was inverted and mixed to form concentrated platelet plasma;
[0047] B. Sonicate the concentrated platelet plasma obtained in step A in an ice bath for 10-15 minutes, and then bathe in liquid nitrogen for 1-2 hours to obtain a platelet plasma lysate;
[0048] C. Take the platelet lysate obtained in step B out of liquid nitrogen and resuscitate, then place it at 18-25°C for 4-6 hours,
[0049] D. Then add heparan sulfate, mix well at 4°C, so that the final concentration of heparan sulfate is 1-3U / ml...
specific Embodiment approach 3
[0057] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the anticoagulant in step A is sodium citrate anticoagulant. Others are the same as in the second embodiment.
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