Method for simultaneously detecting various ethanol non-oxidized metabolites in blood spot
A non-oxidative and metabolite technology, applied in the field of forensic identification, to achieve the effect of improving stability, high sensitivity, easy operation and promotion
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[0033] The chromatographic conditions are as follows:
[0034] Chromatographic column: Thermo Hypersil Gold C18column (2.1mm×100mm, 1.9μm); column temperature: 47°C;
[0035] Mobile phase: 5% acetonitrile-water (phase A); 90% methanol-water (0.1% formic acid) (phase B); methanol (0.1% formic acid) (phase C); isopropanol (5mM ammonium acetate) (phase D ), gradient elution (see Table 3), flow rate 0.2mL / min;
[0036] Injection volume: 5μL
[0037] Table 3 shows the gradient elution conditions;
[0038] Table 3 Gradient elution conditions
[0039]
[0040] Mass Spectrometry Conditions:
[0041] ESI; spray voltage: 3.6kV(+) / 2.8kV(-); sheath gas: 35Arb; auxiliary gas: 10Arb; ion transfer tube temperature: 350°C; desolvation temperature: 300°C;
[0042] Scanning method: multiple reaction monitoring (MRM)
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