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Method for detecting ethyl sulfate and ethyl glucuronate in human urine

A technology of ethyl glucuronate and ethyl sulfate, which is applied in the field of forensic identification, can solve the problems of lower sensitivity of the analyte, affect the accuracy of sensitivity, prolong the detection time, etc., and achieve easy operation and promotion, simple pretreatment process, Reduce the effect of matrix effect

Pending Publication Date: 2021-01-08
FUDAN UNIV
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Problems solved by technology

[0003] At present, there are many methods to detect EtS and EtG in urine, among which liquid phase-tandem mass spectrometry (LC-MS / MS) is the most common; however, due to the large polarity of EtS and EtG Due to its high solubility and water solubility, there is a serious matrix effect in the detection of EtS and EtG in urine by LC-MS / MS, which will directly affect the sensitivity of detection and the accuracy of quantification; Key issues to be solved urgently in EtG detection
At present, the matrix effect can be effectively reduced by simple sample dilution, but the sensitivity of the analyte will also be reduced; or the method of sample pretreatment by solid phase extraction (SPE) or adding acetonitrile after the column can also be used to reduce the matrix effect, but the above method is cumbersome to operate and greatly prolongs the detection time

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  • Method for detecting ethyl sulfate and ethyl glucuronate in human urine
  • Method for detecting ethyl sulfate and ethyl glucuronate in human urine
  • Method for detecting ethyl sulfate and ethyl glucuronate in human urine

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[0026] The chromatographic conditions are as follows:

[0027] Chromatographic column: Phenomenex Synergi 2.5μm Hydro-RP 100A column (2mm×100mm, 2.5μm); column temperature: 40°C;

[0028] Mobile phase: water (0.1% formic acid) (phase A); acetonitrile (0.1% formic acid) (phase B); gradient elution (see Table 3), flow rate 0.25mL / min.

[0029] Injection volume: 5μL

[0030] Table 3 gradient elution conditions

[0031]

[0032] The mass spectrometry conditions are as follows:

[0033] ESI; spray voltage: 3.6kV(+) / 2.8kV(-); sheath gas: 35Arb; auxiliary gas: 10Arb; ion transfer tube temperature: 350°C; desolvation temperature: 300°C.

[0034] Scanning method: multiple reaction monitoring (MRM)

[0035] The chromatographic retention time and mass spectrometry data of the target object were detected, and the results are shown in Table 1.

[0036] Sample handling:

[0037] Drop 20 μL of urine onto Whatman 903# analytical filter paper and microwave for 1 min until dry; cut the...

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Abstract

The invention belongs to the technical field of judicial expertise, and relates to a method for detecting ethyl sulfate and ethyl glucuronate in human urine. The method has the advantages of less detection material consumption, high sensitivity, strong specificity, wide linear range, simple and quick operation, less extraction solvent consumption and short analysis time. The requirements of judicial expertise task urgency and high detection time requirements can be met, and the method can be popularized and applied in a large range; meanwhile, compared with an existing method, the detection method has the advantages that the matrix effect of ethyl sulfate (EtS) and ethyl glucuronate (EtG) in urine can be effectively reduced, and the reliability of a detection result is improved.

Description

technical field [0001] The invention belongs to the technical field of judicial appraisal, and relates to a method for measuring non-oxidative metabolites of ethanol in urine, in particular to a method for simultaneously measuring ethyl sulfate and ethyl glucuronate in human urine; the method uses less material for testing , high sensitivity, strong specificity, small matrix effect, wide linear range, simple and fast operation, and short analysis time, which can meet the urgent task of forensic identification and the demand for high detection time, and can be popularized and applied on a large scale. Background technique [0002] The prior art discloses that ethyl sulfate (EtS) and ethyl glucuronate (EtG) are non-oxidative metabolites of ethanol, produced by phase II metabolism of ethanol, and are biomarkers for judging ethanol intake. Due to the high specificity and long detection time limit, in general, EtS and EtG in urine can be used to judge recent drinking behavior in ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 饶渝兰王昊樊恩杉
Owner FUDAN UNIV
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