Method for simultaneously detecting 4 species of ethyl alcohol non-oxide metabolins in human whole blood
A non-oxidative and metabolite technology, applied in the field of forensic identification and analytical chemistry, can solve the problems of large differences in the physical and chemical properties of the four types of non-oxidative metabolism, and achieve the effect of easy operation and promotion, high difficulty and good accuracy
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[0030] Example 1 Detection of 4 types of ethanol non-oxidative metabolites in human whole blood
[0031] The chromatographic conditions are as follows:
[0032] Chromatographic column: Thermo Hypersil Gold C18 column (2.1mm×100mm, 1.9μm); column temperature: 47°C
[0033] Mobile phase: 5% acetonitrile-water (phase A); 90% methanol-water (0.1% formic acid) (phase B); methanol (0.1% formic acid) (phase C); isopropanol (5mM ammonium acetate) (phase D ) gradient elution (as shown in Table 3), flow rate 0.2mL / min.
[0034] Injection volume: 5μL
[0035] Table 3 Gradient elution conditions
[0036]
[0037] The mass spectrometry conditions are as follows:
[0038] ESI; spray voltage: 3.6kV(+) / 2.8kV(-); sheath gas: 35Arb; auxiliary gas: 10Arb; ion transfer tube temperature: 350°C; desolvation temperature: 300°C.
[0039] Scanning method: multiple reaction monitoring (MRM)
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