Biological wetting reversal agent for high-temperature high-salt heavy oil reservoir, and preparation method thereof
A wetting inversion, heavy oil reservoir technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor temperature and salt resistance, and achieve strong temperature and salt resistance, construction The process is simple and ensures the effect of competitive adsorption advantages
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Embodiment 1
[0034] Example 1 biological wetting reversal agent D 1 and its preparation
[0035] Biological Wetting Reversal Agent D 1 The composition and components are as follows:
[0036]
[0037] The rhamnolipid fermentation broth is a fermentation broth of Pseudomonas aeruginosa, and its concentration is greater than 35g / L.
[0038] The sophorolipid fermentation liquid is a yeast fermentation liquid, and its concentration is greater than 100g / L.
[0039] The alkyl polyglycoside is a biological anionic nonionic surfactant with a relative molecular weight of 290-370.
[0040] Described alcohol auxiliary agent is butanol.
[0041] Biological Wetting Reversal Agent D 1 Preparation of:
[0042] (1) Insert Pseudomonas aeruginosa seed liquid into the sterilized culture medium by 10%, the fermentation condition is 37°C and 180r / min, and ferment for 2 days to obtain rhamnolipid fermentation liquid;
[0043] (2) Insert the yeast seed liquid into the sterilized culture medium by 10%, t...
Embodiment 2
[0047] Example 2 Biological wetting reversal agent D 2 and its preparation
[0048] Biological Wetting Reversal Agent D 2 The composition and components are as follows:
[0049]
[0050] The rhamnolipid fermentation broth is a fermentation broth of Pseudomonas aeruginosa, and its concentration is greater than 35g / L.
[0051] The sophorolipid fermentation liquid is a yeast fermentation liquid, and its concentration is greater than 100g / L.
[0052] The alkyl polyglycoside is a biological anionic nonionic surfactant with a relative molecular weight of 290-370.
[0053] Described alcohol auxiliary agent is amyl alcohol.
[0054] Biological Wetting Reversal Agent D 2 Preparation of:
[0055] (1) Insert Pseudomonas aeruginosa seed liquid into the sterilized culture medium by 10%, the fermentation condition is 38°C and 200r / min, and ferment for 2 days to obtain rhamnolipid fermentation liquid;
[0056] (2) Insert the yeast seed liquid into the sterilized culture medium by 1...
Embodiment 3
[0060] Example 3 Biological Wetting Reversal Agent D 3 and its preparation
[0061] Biological Wetting Reversal Agent D 3 The composition and components are as follows:
[0062]
[0063] The rhamnolipid fermentation broth is a fermentation broth of Pseudomonas aeruginosa, and its concentration is greater than 35g / L.
[0064] The sophorolipid fermentation liquid is a yeast fermentation liquid, and its concentration is greater than 100g / L.
[0065] The alkyl polyglycoside is a biological anionic nonionic surfactant with a relative molecular weight of 290-370.
[0066] Described alcohol auxiliary agent is hexanol.
[0067] Biological Wetting Reversal Agent D 3 Preparation of:
[0068] (1) Insert Pseudomonas aeruginosa seed liquid into the sterilized culture medium by 10%, the fermentation condition is 40° C. and 250 r / min, and ferment for 3 days to obtain rhamnolipid fermentation liquid;
[0069] (2) Insert the yeast seed liquid into the sterilized culture medium by 10%...
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