Penaeus monodon Na<+>/K<+>/2Cl<-> cotransporter NKCC gene and application thereof

A co-transporter, Penaeus monodon technology, applied in the field of molecular biology, can solve the problems of insufficient metabolic function and lack of key transporters
CN110684776AActive Publication Date: 2020-01-14SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2020-01-14

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Abstract

The invention discloses a penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene. An alkali base sequence of the penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene is as shown in SEQ ID NO:1. The invention further discloses a coding protein of the penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene. An amino acid sequence of the coding protein is as shown in SEQ ID NO: 2. The invention further discloses application of the penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene or the coding protein in improving the low salt tolerance of penaeus monodon and SNP loci related to low salt tolerance of the penaeus monodon. The SNP loci are the 2061<st>and / or the 2157 loci of the alkali base sequence as shown in the SEQ ID NO: 1, and an alkali base mutates into A from T. Important reference marks are provided for selecting and breeding molecules of new species of low salt tolerance of the penaeus monodon.
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Description

Technical field

[0001] The invention belongs to the technical field of molecular biology, and specifically relates to a Penaeus monodon Na + / K + / 2Cl - Cotransporter NKCC gene and its application. Background technique

[0002] Na + / K + / 2Cl - Cotransporter (Na + / K + / 2Cl - -cotransporter, NKCC) is a type of ion transmembrane transport protein that exists widely in animals, encoded by SLC12A family genes, responsible for the transmembrane transport of Na, K, and Cl ions. It is an electrically neutral ion transmembrane transporter that transports in the ratio of 1Na:1K:2Cl. It is divided into two subtypes of NKCC1 and NKCC2 in vertebrates, while only one type of NKCC is found in crustaceans.

[0003] At present, a lot of progress has been made in the research of NKCC in aquatic animals. Among aquatic animals, NKCC1 was first found in the rectal glands of soft-shelled turtles; in 2002, Cutler et al. cloned the NKCC1a and NKCC1b sequences in European eels (Anguilla anguilla); in 20...

Claims

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