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Penaeus monodon Na<+>/K<+>/2Cl<-> cotransporter NKCC gene and application thereof

A co-transporter, Penaeus monodon technology, applied in the field of molecular biology, can solve the problems of insufficient metabolic function and lack of key transporters

Active Publication Date: 2020-01-14
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The salinity in the water environment will show obvious diurnal fluctuations and seasonality under natural conditions. In addition, severe weather such as heavy rains and typhoons and water changes in aquaculture production will also change the salinity in the water environment. This acute salinity However, the research on the response of Penaeus monodon to low-salt stress and the metabolic function under low-salt stress is not deep enough, especially the key transporter that plays a role in material transfer during the process of low-salt stress. Protein research is still lacking

Method used

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  • Penaeus monodon Na&lt;+&gt;/K&lt;+&gt;/2Cl&lt;-&gt; cotransporter NKCC gene and application thereof
  • Penaeus monodon Na&lt;+&gt;/K&lt;+&gt;/2Cl&lt;-&gt; cotransporter NKCC gene and application thereof
  • Penaeus monodon Na&lt;+&gt;/K&lt;+&gt;/2Cl&lt;-&gt; cotransporter NKCC gene and application thereof

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Embodiment 1

[0027] 1. Experimental materials

[0028] 1.1 Experimental animals

[0029] Penaeus monodon was taken from the Shenzhen Experimental Base of the South China Sea Fisheries Research Institute. The prawns with a body length of 8±2cm and a body weight of 10±2g were temporarily cultured in a cement pond (4.71m×4.13m×1.73m) in natural seawater. 5.84m 3 ), and fully aerated, the breeding temperature is 27±2℃. The average weight of Penaeus monodon used for dsRNA injection is 5±1g.

[0030] 1.2 Experimental instruments and experimental reagents

[0031] Use conventional experimental instruments and experimental reagents in the field, commercially available instruments or reagents.

[0032] 2. Experimental method

[0033] 2.1 Extraction of total RNA and construction of cDNA library

[0034] Randomly select 3 healthy and complete female and male prawns from the temporarily reared prawns, and take tissue samples of hepatopancreas, gills, heart, intestine, stomach, lymph, epidermis, muscle, stalk ne...

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Abstract

The invention discloses a penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene. An alkali base sequence of the penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene is as shown in SEQ ID NO:1. The invention further discloses a coding protein of the penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene. An amino acid sequence of the coding protein is as shown in SEQ ID NO: 2. The invention further discloses application of the penaeus monodon Na<+> / K<+> / 2Cl<-> cotransporter NKCC gene or the coding protein in improving the low salt tolerance of penaeus monodon and SNP loci related to low salt tolerance of the penaeus monodon. The SNP loci are the 2061<st>and / or the 2157 loci of the alkali base sequence as shown in the SEQ ID NO: 1, and an alkali base mutates into A from T. Important reference marks are provided for selecting and breeding molecules of new species of low salt tolerance of the penaeus monodon.

Description

Technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a Penaeus monodon Na + / K + / 2Cl - Cotransporter NKCC gene and its application. Background technique [0002] Na + / K + / 2Cl - Cotransporter (Na + / K + / 2Cl - -cotransporter, NKCC) is a type of ion transmembrane transport protein that exists widely in animals, encoded by SLC12A family genes, responsible for the transmembrane transport of Na, K, and Cl ions. It is an electrically neutral ion transmembrane transporter that transports in the ratio of 1Na:1K:2Cl. It is divided into two subtypes of NKCC1 and NKCC2 in vertebrates, while only one type of NKCC is found in crustaceans. [0003] At present, a lot of progress has been made in the research of NKCC in aquatic animals. Among aquatic animals, NKCC1 was first found in the rectal glands of soft-shelled turtles; in 2002, Cutler et al. cloned the NKCC1a and NKCC1b sequences in European eels (Anguilla anguilla); in 20...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435C12Q1/6888
CPCC07K14/43504C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 周发林李运东江世贵何鹏杨其彬姜松杨丽诗黄建华
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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