Application of stem cell exosomes to preparation of product capable of promoting wound healing

A technology for wound healing and exosomes, applied in the field of medicine, to achieve the effects of convenient storage, elimination of limitations, and mature extraction methods

Inactive Publication Date: 2020-01-17
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Stem cell therapy has opened up a new perspective in regenerative medicine and wound management. Although stem cells have made impo

Method used

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  • Application of stem cell exosomes to preparation of product capable of promoting wound healing
  • Application of stem cell exosomes to preparation of product capable of promoting wound healing
  • Application of stem cell exosomes to preparation of product capable of promoting wound healing

Examples

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Embodiment 1

[0034] Example 1 Application of Umbilical Cord Mesenchymal Stem Cells (huc-MSCs) Exosomes in Wound Healing

[0035] 1. Extraction and identification of exosomes from umbilical cord mesenchymal stem cells (huc-MSCs)

[0036] 1. Preparation of umbilical cord mesenchymal stem cells

[0037] The umbilical cord of the newborn is provided by the obstetrics department during caesarean section (gestational age 37-40 weeks). The obtained umbilical cord is washed with normal saline, the residual blood of the umbilical cord is removed, and it is cut into 5 cm sections. Each section is cut along the vein cavity and removed after being tiled For the vein, cut deeply and remove 2 arteries, take the jelly between the blood vessels and between the blood vessels and the adventitia and cut them into small pieces of 1mm or less, wash them with balanced salt solution, and soak them in a concentration of 0.2 % collagenase solution, placed in a 37°C water bath for 17 hours to digest the tissue blo...

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Abstract

The invention discloses application of stem cell exosomes to preparation of a product capable of promoting wound healing, firstly provides application of the stem cell exosomes to preparation of the product capable of promoting wound healing, and further provides the product capable of promoting wound healing. The stem cell exosomes are free of immunogenicity and tumorigenicity, an extraction method is mature, preservation is convenient, the stem cell exosomes are attached to the skin with pressure sores, the content of myocollagen fibers in wound tissue and the expression quantity of I-type collagens and/or III-type collagens and/or HMGB1 and/or TGF-beta are decreased, and thus wound healing is promoted. The exosomes are used for substituting stem cells subjected to strict clinical use caused by the reasons of immunogenicity and tumorigenicity, the limitation of stem cell treatment is eliminated, and the corresponding treatment effect can be achieved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the application of stem cell exosomes in the preparation of products for promoting wound healing. Background technique [0002] Stem cell therapy has opened up a new perspective in regenerative medicine and wound management. Although stem cells have made important progress in regenerative medicine and tissue engineering, wound healing remains a challenging clinical problem with some limitations. . [0003] Exosomes are small membrane vesicles (30-150nm) that contain complex RNA and proteins. The application of mesenchymal stem cells can improve diseases, and paracrine and immune regulation pathways are the main mechanisms of its in vivo effects. A large number of studies have demonstrated the potential of exosomes as therapeutic carriers for stem cell therapy. Exosomes can be used by cells as messengers and carriers to transmit signals to other cells, alter their function in n...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P17/02
CPCA61K35/28A61P17/02
Inventor 李富荣于丽闫飞
Owner SHENZHEN PEOPLES HOSPITAL
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