Rapid identification method for soybean varieties with different phosphorus efficiency genotypes

An identification method and genotype technology, applied in the field of rapid identification of soybean varieties with different phosphorus efficiency genotypes, can solve the problems of long test period, heavy workload, slow test progress, etc., so as to reduce the overall screening workload and work cycle. Effect

Inactive Publication Date: 2020-01-17
SHENYANG AGRI UNIV
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Problems solved by technology

[0006] For this reason, the embodiment of the present invention provides a rapid identification method for soybean varieties with different phosphorus efficiency genotypes, so as to solve the problems of heavy workload and long test period in the screening process of existing phosphorus-efficient soybean varieties with yield traits as identification indicators. Slow test progress and other issues

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  • Rapid identification method for soybean varieties with different phosphorus efficiency genotypes
  • Rapid identification method for soybean varieties with different phosphorus efficiency genotypes
  • Rapid identification method for soybean varieties with different phosphorus efficiency genotypes

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Experimental program
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Embodiment 1

[0028] 1 cultivated soybean

[0029] Adopt sand cultivation method to cultivate soybeans, and the cultivation plate used is made of PVC pipe, with an internal diameter of 16 cm and a height of 25 cm. Three small holes with a diameter of 0.2 cm are drilled at the bottom of the basin. The quartz sand after every basin is filled to the basin mouth 3-4cm place (the weight of quartz sand is about 5kg). Two treatments of normal phosphorus and low phosphorus were set up for each variety. Potassium dihydrogen phosphate (KH 2 PO 4 ), repeated three times, and randomized blocks.

[0030] Sow five seeds per pot, pour 500mL distilled water at 8:00 every day, and pour 1000mL distilled water at 4:00 p.m. Pour 500mL of semi-nutrient solution every hour, and pour 1000mL of distilled water at 4 o'clock in the evening. After one week, change the semi-nutrient solution to full nutrient solution until the soybeans mature.

[0031] The phosphorus concentration in the nutrient solution used fo...

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Abstract

The embodiment of the invention discloses a rapid identification method for genotype soybean varieties with different phosphorus efficiency genotypes, and belongs to the technical field of soybean breeding. The method comprises the following steps: (1) after soybean seeds to be detected germinate into buds, respectively carrying out low-phosphorus treatment and normal-phosphorus treatment on eachvariety; (2) measuring the root surface area and the root diameter of the soybeans in the seedling stage under low-phosphorus treatment and the relative value of the phosphorus utilization efficiencyof the roots in the seedling stage of the soybeans; and (3) substituting into a regression equation, carrying out systematic clustering on a D value, and identifying the phosphorus high-efficiency genotype soybean variety, the phosphorus low-efficiency genotype soybean variety and the intermediate type soybean variety according to a clustering result. According to the method, the phosphorus efficiency of the genotype soybean varieties can be rapidly identified through three indexes in the seedling stage, the overall screening workload can be reduced, the working period can be shortened, and afoundation is laid for rapid screening of the genotype soybean varieties with different phosphorus efficiencies.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of soybean breeding, in particular to a rapid identification method for soybean varieties with different phosphorus efficiency genotypes. Background technique [0002] Soybean is a phosphorus-loving crop, and phosphorus is one of the important factors affecting the growth and yield of soybean. In my country, about 50% of cultivated land soil available phosphorus <10mgP / kg. According to research, 43% of the 1.37 billion hectares of cultivated land in the world are deficient in phosphorus, while 2 / 3 of the 107 million hectares of cultivated land in my country are deficient in phosphorus. Therefore, phosphorus deficiency is a common problem in the world and a major obstacle restricting agricultural production. factor. Phosphorus fertilizer application is currently the most common way to solve soil phosphorus deficiency, but excessive fertilization and inappropriate fertilization me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K9/62G06Q10/06G06Q50/02A01C21/00
CPCG06Q10/0639G06Q50/02A01C21/00G06F18/24323
Inventor 敖雪王辉谢甫绨赵宏宇张惠君王海英姚兴东
Owner SHENYANG AGRI UNIV
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