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Probiotic composition as well as preparation and application of probiotic composition

A probiotic and composition technology, applied in the field of probiotic preparations, can solve problems such as unclear mechanism, and achieve the effect of alleviating RSV virus infection

Active Publication Date: 2020-01-24
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic scholars have found that probiotics have a good effect in the treatment of infantile bronchopneumonia, but the mechanism is not clear

Method used

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  • Probiotic composition as well as preparation and application of probiotic composition
  • Probiotic composition as well as preparation and application of probiotic composition
  • Probiotic composition as well as preparation and application of probiotic composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] The present invention can be better understood through the following experimental procedures.

[0046] (1) The experimental process is as follows:

[0047] (1) RSV virus amplification Use RPMI1640 containing 10% fetal bovine serum as the RSV maintenance solution, and the human laryngeal carcinoma cells (Hep-2) passed to the second generation are RSV reproductive cells, and the Hep-2 cells grow and fuse until dense Virus amplification experiments were performed after the monolayer. Discard the cell culture medium before amplification, wash with PBS buffer three times, inoculate 5 mL of RSV-A type A2 standard strain, 37°C, 5% CO 2 Adsorption under constant temperature conditions for 2h. After the adsorption was completed, the virus liquid was discarded, and 30 mL of RPMI1640 maintenance solution with 10% fetal bovine serum was added for cultivation. Observe day by day, when more than 80% of the cells have lesions, the cells are ultrasonically disrupted, centrifuged at ...

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PUM

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Abstract

The invention discloses a probiotic composition as well as a preparation and application of the probiotic composition. The probiotic composition comprises VSL#3, lactobacillus rhamnosus M9 and lactobacillus plantarum P-8 which are mixed in an equal ratio of 1:1:1. The probiotic composition is applied to preparation of a drug for treating or preventing pneumonia, particularly pneumonia caused by respiratory syncytial virus (RSV) infection and infantile bronchopneumonia caused by RSV infection. The probiotic composition can relieve change of lung tissue inflammatory pathologic structures causedby RSV infection and inhibit virus infection process of RSV infection, activate TLR3 and RIG-1 signal pathways in alveolar macrophages to promote production of IFN-beta to inhibit expression of lung inflammation factors, and regulate lung florae and intestinal florae to relieve RSV infection.

Description

technical field [0001] The invention belongs to the field of probiotic preparations, and in particular relates to a probiotic composition and its preparation and application. Background technique [0002] Probiotics are a class of active microorganisms that can promote the balance of intestinal microbial flora and have beneficial effects on the health or physiological functions of the host. At present, it is widely used in the fields of life and health, scientific research, bioengineering, industry and agriculture, and food safety. A large number of domestic and foreign studies have shown that probiotics have positive effects in lowering blood pressure, lowering blood sugar, lowering blood fat, anti-allergy, anti-inflammation, regulating immunity, maintaining the balance of intestinal flora, etc., and have very broad application prospects in functional foods and medicines . Probiotics can play an immunomodulatory role in various ways. For example, probiotics can directly e...

Claims

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Application Information

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IPC IPC(8): A61K35/747A23L33/135A61P11/00A61P31/14A61K35/741
CPCA61K35/747A61K35/741A61P31/14A61P11/00A23L33/135A23V2002/00A61K2300/00A23V2200/314A23V2400/175A23V2400/169
Inventor 吕志刚纪建建孙沁梅王倩
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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