Method for preparing chebulagic acid by ethanol reflux method

Abstract

The invention provides a method for preparing chebulagic acid by an ethanol reflux method. The method comprises the following steps of (1) preparing an extracting solution: performing reflux extraction on fructus phyllanthi medicinal materials with ethanol so as to obtain the extracting solution; (2) preparing a concentrated solution: concentrating the extracting solution until no ethanol taste exists; (3) preparing a sample feeding solution: adding water to the concentrated solution, performing dispersing, and performing centrifuging or filtration so as to obtain supernatant namely the samplefeeding solution; (4) performing macroporous resin enrichment: enabling the sample feeding solution to be subjected to macroporous resin adsorption, and performing eluting; (5) performing purification: enabling eluent obtained in the step (4) to be subjected to column chromatography; and (6) performing drying: drying the eluent obtained in the step (5). The method is simple and convenient, the content and the transfer rate are high, the amplification technology verification result also indicates that the established preparation technology is stable and feasible, and the method is suitable forindustrial production requirements.

Description

technical field [0001] The invention relates to a preparation method of active ingredients of traditional Chinese medicine, in particular to a method for preparing challelac by reflux of ethanol. Background technique [0002] Amla emblica Phyllanthus emblica The fruit of L. has the effects of resolving phlegm, promoting body fluid, relieving cough and detoxification. It is mainly used to treat cold and fever, cough and sore throat, diphtheria, restlessness and dry mouth. Distributed in Fujian, Guangdong, Guangxi, Yunnan, Guizhou, Sichuan, Taiwan and other places. Most of emblica are wild or semi-wild, almost free from industrial pollution, and are veritable raw materials for green food processing. [0003] The fresh fruit of Amla emblica contains 45% tannin, the immature fruit contains 30% to 35% tannin, and the total tannin content in dried fruit can reach 14%, of which chebrinic acid and chebrinic acid can be separated (chebulagic acid), corilagin, terchebin, chebulic ...

AI Technical Summary

Problems solved by technology

[0004] At present, there are few domestic researches on chrysalis acid, which is still in its infancy. CN104945447A, disclosed on September 30, 2015, relates to the preparation method of chellia acid. Extraction, ceramic membrane separation, macroporous resin adsorption and other steps, such as enzymatic hydrolysis and ultrasonic extraction are not widely used in production, which is not conducive to industrialization promotion, and the content is low and the cost is high