A kind of tuna white meat ACE inhibitory peptide and preparation method thereof
A technology for inhibiting peptide and tuna, applied in the field of tuna white meat ACE inhibitory peptide and its preparation, can solve the problems of high cost of blood pressure-lowering peptide, unsuitable for industrial production, low antihypertensive activity, etc., and achieves good inhibitory activity, low preparation cost, Efficient effect
Active Publication Date: 2021-07-13
ZHEJIANG OCEAN UNIV
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Problems solved by technology
[0003] In order to overcome the problems of high cost, low efficiency, inapplicability to industrial production, and low antihypertensive activity of existing antihypertensive peptides, the present invention proposes a tuna white meat ACE inhibitory peptide and a preparation method thereof
Method used
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Embodiment 1-15
[0041] Example 1-15: A method of preparing a tuna white meat ACE inhibits peptide, including the following steps:
[0042] 1) Tone tuna white meat, dry and remove the water, then add ethyl acetate, immersion degreasing, rotary evaporation, dry, and pulverize the spare;
[0043] 2) Add the pre-treated white meat powder to distilled water, and after adjusting the pH to 8.5-10.5, 1-3 wt% alkaline protease is added, and the enzymatic hydrolysis is obtained at 45-65 ° C to obtain an enzymatic flu fluid;
[0044] 3) Heat the enzymatic hydrolyzate at a water bath at a water bath for 10 min for the treatment, then centrifuge at 4000 R for 20 min, take the supernatant, the enzyme solution, and then freeze, to obtain polypeptide powder, measure ACE inhibitory activity;
[0045]4) Ultrafiltrate the tuna white meat solution using 3.5 kda ultrafiltration membrane, and the product component is crystallized to obtain an enzymatic and hydrolyzate powder; then the enzymatic solution and ultrapure ...
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Abstract
A tuna white meat ACE inhibitory peptide and a preparation method thereof, relating to the field of ACE inhibitory peptide preparation, the peptide amino acid sequence of the tuna white meat ACE inhibitory peptide is Ser-Pro or Val-Asp-Arg-Tyr-Phe, comprising the following preparation steps: 1 ) Thaw the white meat of tuna, dry it to remove water, then degrease and dry it; 2) add the pretreated white meat powder to distilled water, adjust the pH value, add protease for enzymolysis, and obtain an enzymatic solution; 3) add the enzyme The hydrolyzate was subjected to enzyme-inactivating treatment, and the supernatant was obtained by centrifugation to obtain an enzyme-inactivating hydrolyzate, which was freeze-dried to obtain polypeptide powder for measuring ACE inhibitory activity; Purified by liquid chromatography and amino acid sequencing, the ACE inhibitory peptide of tuna white meat is obtained. The ACE inhibitory peptide of tuna white meat prepared by the invention has good inhibitory activity, low preparation cost and high efficiency, and is suitable for industrial production.
Description
Technical field [0001] The present invention relates to the preparation of ACE inhibitory peptides, and more particularly to a tuna white meat ACE inhibitory peptide and a preparation method thereof. Background technique [0002] Hypertensive pressure is characterized by high blood pressure or (and) diastolic pressure, and leads to systemic diseases such as stroke, myocardial infarction, heart failure, dementia, liver and kidney failure, blindness, etc., has become my country and the world an important public health issue. The blood pressure adjustment of the human body is related to a variety of nerves, such as sympathetic system, renin-angiotensin system, ras, kallikrein-kinin system, kks) , Kidney and body fluid balancing system and nitric oxide-endothelin system. Among them, renin-angiotensin systems and kinacin-release enzyme-kini-free systems play an important role in the regulation of blood pressure. RAS and KKS two systems interact and achieve the adjustment of blood pres...
Claims
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IPC IPC(8): C07K5/062C07K7/06A61P9/12C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16
CPCA61P9/12C07K5/06069C07K7/06C12P21/06
Inventor 赵玉勤
Owner ZHEJIANG OCEAN UNIV