Fluorescent quantitative PCR detection method of human coronavirus and respiratory syncytial virus and application thereof

A human coronavirus, fluorescence quantitative technology, applied in the field of fluorescence quantitative PCR, to achieve good sensitivity and specificity, good reproducibility
CN110724764AInactive Publication Date: 2020-01-24INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
Publication Date
2020-01-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides primers and probes or a combination thereof of fluorescent quantitative PCR that can be used to detect diseases caused by human coronavirus and respiratory syncytial virus. In aspecific embodiment, the sequences of the primers and probes are as shown in SEQIDNOs: 1-3, and / or as shown in SEQIDNOs: 4-6, and / or as shown in SEQIDNOs: 7-9. The invention also provides applicationof the primers and probes or the combination thereof to preparation of reagents for detecting human coronavirus OC43, human coronavirus HKU1 and / or respiratory syncytial virus RSVB in samples.
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Description

technical field

[0001] The invention relates to the field of human respiratory virus detection, in particular to a fluorescent quantitative PCR method and application thereof which can be used in the field of detection of diseases caused by human coronavirus and respiratory syncytial virus. Background technique

[0002] At present, infection is still a major threat to human health, especially acute respiratory infectious diseases, which have high morbidity and mortality due to their many types, rapid spread, and wide prevalence, posing a major threat to human health, social stability, and economic development. . The Global Burden of Diseases, Injuries and Risk Factors (GBD) study found that lower respiratory tract infections killed 650,000 children under 5 years of age and 1.08 million adults over 70 years of age in 2016, out of an estimated 2.38 million deaths worldwide in all age groups lower respiratory infection, making it the sixth leading cause of death in all age gro...

Claims

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