Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of NRT2_5 protein to adjustment and control of width of plant subtended leaves

A protein and plant technology, applied in the application field of NRT2_5 protein in regulating the width of plant bracts, can solve the problem of slow dehydration rate of grain

Active Publication Date: 2020-01-31
CHINA AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dehydration rate is negatively correlated with the degree of bract leaf coverage. Yan Shuqin et al. (2007) measured 9 maize inbred lines and their hybrid combinations. Related; the number of bract leaves is large, the area is large, and the water content is high, the dehydration rate of the grain is slow

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of NRT2_5 protein to adjustment and control of width of plant subtended leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the discovery of NRT2_5 gene

[0059] 1. In 2017, the inventor of the present invention sowed 500 related populations of maize inbred lines with extensive variation in Tieling, Liaoning, sowing 10 seeds in each row, and repeating in three plots. Measure the bract width with a soft rope ruler.

[0060] 2. After step 1 was completed, a mixed linear model (MLM) was used to conduct GWAS analysis on the BLUP values ​​of the bract width of 442 materials, and then a gene that might be related to the maize bract width was found, which was named NRT2_5 gene.

[0061] The NRT2_5 gene is located on chromosome 8 of maize, and its nucleotide sequence is shown in SEQ ID NO:1. The nucleotide sequence of the cDNA of the NRT2_5 gene is shown in SEQ ID NO: 2, which encodes the NRT2_5 protein shown in SEQ ID NO: 3.

Embodiment 2

[0062] Embodiment 2, the application of NRT2_5 gene in the regulation and control maize bract leaf width

[0063] A. Application one

[0064] In this example, a target was selected for the experiment, and the target sequence was: 5'-ATGTTCTCGCCGCCCAAGG-3' (ie, the 760-778th position from the 5' end of SEQ ID NO: 1), corresponding to the target gene NRT2_5 gene.

[0065] 1. Synthesis of sgRNA

[0066] Design and artificially synthesize sgRNA, the nucleotide sequence of sgRNA is: 5'-GUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC-3' (SEQ ID NO: 5). The target recognized by sgRNA is the DNA molecule shown in positions 760-778 from the 5' end of SEQ ID NO: 1.

[0067] The coding gene of sgRNA is:

[0068] 5'-GTTTTAGGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC-3' (SEQ ID NO: 6).

[0069] Second, the acquisition of recombinant plasmids

[0070] 1. Synthetic primer NRT2_5-F: 5'-GGCG ATGTTCTCGCCGCCCAAGG -3' (the underline is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of NRT2_5 protein to adjustment and control of width of plant subtended leaves. The amino acid sequence of NRT2_5 protein is shown as SEQID NO:3. Experiment verifies that the expression level and / or activity of the NRT2_5 protein in corn B104 are / is reduced, and transgenosis corn is obtained. Compared with the corn B104, the transgenosis corn has the advantage that the width of the subtended leaves is notably reduced. Therefore, the NRT2_5 protein can adjust and control the width of the plant subtended leaves. The NRT2_5 protein has important applicationvalue.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of NRT2_5 protein in regulating the width of plant bracts. Background technique [0002] The photosynthesis of maize bracts plays an important role in the formation of ear grain yield. Corn Bract No C 4 The rosette structure of plant leaves, but the photosynthetic carbon assimilation pathway has both C3 and C4 pathways. Yakir et al. (1991) conducted a quantitative analysis of carbohydrate sources in maize husks using isotope labeling. The results showed that 16% of the cellulose synthesis in maize husks came from the husk C3 pathway, 62% came from the C4 photosynthetic pathway, and the remaining 22% came from the C4 pathway. Transported from sucrose synthesized by other leaves. Although the husk area and the stem-leaf area of ​​corn are relatively small, if the husk leaves are removed, the corn yield will be reduced to a certain extent. Jain (1971) als...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/82A01H5/12A01H6/46
CPCC07K14/415C12N15/8261
Inventor 贺岩王琪夏嫒嫒崔震海王淄
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com