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Double-layer micro-fluidic chip for capturing circulating tumor cells

A microfluidic chip and tumor cell technology, applied in the field of double-layer microfluidic chips, can solve the problem of difficult to achieve precise capture of tumor cells circulating in the blood, and achieve the effects of avoiding being missed, efficient capture, and improving the capture rate

Pending Publication Date: 2020-02-07
ANHUI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the microfluidic chip in this scheme does not use antibodies, it only adopts a single physical scale screening method, so it is difficult to achieve precise capture of circulating tumor cells in the blood.

Method used

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  • Double-layer micro-fluidic chip for capturing circulating tumor cells
  • Double-layer micro-fluidic chip for capturing circulating tumor cells
  • Double-layer micro-fluidic chip for capturing circulating tumor cells

Examples

Experimental program
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Effect test

Embodiment 1

[0049] combine Figure 1 to Figure 4 , a double-layer microfluidic chip for capturing circulating tumor cells in this embodiment, including an upper layer chip and a lower layer chip that are arranged correspondingly up and down and connected to each other. The upper layer chip extends outward from the center of the circle along the radial direction to form multiple clusters of the upper layer Channel 100, the upper layer chip is provided with an upper layer inlet 112 at the center of the circle, and an upper layer outlet 113 is arranged at the circumference. The upper layer inlet 112 and the upper layer outlet 113 are connected to the upper layer channel 100 respectively. The pillar array 200 forms micropillar channels for trapping circulating tumor cells, and the width of the micropillar channels is smaller than the diameter of circulating tumor cells.

[0050] Specifically in this embodiment, the upper micropillar array 200 includes arc-shaped micropillars 210, rectangular ...

Embodiment 2

[0066] combine Figure 5 and Figure 6 , Figure 5 It is a schematic diagram of the internal structure of the lower layer chip. A double-layer microfluidic chip for capturing circulating tumor cells in this embodiment has basically the same structure as that in Embodiment 1. Further, the lower layer micropillar array 400 in this embodiment includes Bowl-shaped structures 420 are distributed in an array at equal intervals along the extending direction of the lower channel 300. The bowl-shaped structures 420 are provided with two notches 421 on the side of the inner side 312 of the lower channel. The radius of curvature of the notches 421 is 30 microns. The gap 421 of the structure 420 forms a capture cavity 422 for enriching circulating tumor cells; the bowl-shaped structure 420 is basically parallel to the lower channel 300, between the bowl-shaped structure 420 and the inner side 312 of the lower channel, and the bowl-shaped structure 420 and the outer side 313 of the lower ...

Embodiment 3

[0069] The structure of a double-layer microfluidic chip for capturing circulating tumor cells in this embodiment is basically the same as that in Embodiment 1. Furthermore, in this embodiment, the lower micropillar array 400 also includes at least two groups along the lower channel 300. The trapezoidal micropillars 410 arranged in the extending direction, each group of trapezoidal micropillars 410 are arrayed from the sidewall of the channel to the center of the channel and meet at the center of the channel. A bowl-shaped structure 420 is provided at the intersection.

[0070] combine Figure 7 and Figure 8 , Figure 7 It is another schematic diagram of the internal structure of the lower chip. Specifically, in this embodiment, the lower microcolumn array 400 includes four sets of trapezoidal microcolumns 410 arranged along the extending direction of the lower channel 300. Every two groups of trapezoidal microcolumns 410 extend from the channel side walls The direction of ...

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Abstract

The invention discloses a double-layer micro-fluidic chip for capturing circulating tumor cells, and belongs to the technical field of micro-fluidic. The double-layer micro-fluidic chip comprises an upper-layer chip and a lower-layer chip which are correspondingly arranged up and down and are communicated with each other, and micro-column arrays are arranged in the upper-layer chip and the lower-layer chip so as to capture the tumor cells for multiple times; and a bowl-shaped structure is also arranged in the lower-layer chip to gather the tumor cells, so that signals are enhanced when characteristic spectrums of enriched CTCs are detected by laser, and the tumor type and gene protein spectral characteristics of a tumor patient can be obtained. According to the double-layer micro-fluidic chip, the defect of low tumor cell capture rate in the prior art is overcome, the structural design is ingenious, capture methods of circulating the tumor cells can be combined and selected according to requirements, the capture rate of CTCs is increased, and missing of individual tumor cells is avoided.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and more specifically relates to a double-layer microfluidic chip for capturing circulating tumor cells. Background technique [0002] Cancer is the number one disease threatening human health. In 2018, there were 18.1 million new cancer cases and 9.6 million deaths worldwide, further increasing the global cancer burden. Lung cancer, breast cancer, and colon cancer rank among the top three in the incidence of cancer worldwide, and they rank first, fifth, and second in the death rates of various types of cancer, respectively. Taken together, the morbidity and mortality of these three types of cancer account for 1 / 3 of the global cancer. 90% of cancer patients die from cancer metastasis. Overcoming cancer is the goal that human beings are striving for. Traditional resection, chemotherapy, radiation therapy and other methods are costly, bring great pain to patients and have recurrence. Circ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/25G01N33/569C12N5/09
CPCB01L3/502707B01L3/502761B01L2200/0647C12N5/0693C12N2509/00G01N21/25G01N33/56966
Inventor 陈红梅刘银年李庆利焦晓栋刘国庆王双寿贾虎任福刚李勇
Owner ANHUI UNIVERSITY OF TECHNOLOGY
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