Method for detecting active ingredients of salviae miltiorrhizae and products

A technology of active ingredients and detection methods, which is applied in the field of quality detection of Chinese medicinal materials and their preparations, can solve the problems of single detection index, unfavorable quality control of Zidanshen medicinal materials and Zidan Huoxue series preparations, etc.

Pending Publication Date: 2020-02-07
YUNNAN CHUXIONG TIANLI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to comprehensively control the quality of Zidanshen medicinal material and Zidan Huoxue series preparations, the technical problem to be solved by the present invention is that in the prior art, the purple Danshen medicinal material and its Zidan Huoxue series preparations have a singl

Method used

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  • Method for detecting active ingredients of salviae miltiorrhizae and products
  • Method for detecting active ingredients of salviae miltiorrhizae and products
  • Method for detecting active ingredients of salviae miltiorrhizae and products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] This example is aimed at the detection method of the active ingredients of the purple salvia miltiorrhiza produced in Chuxiong, Yunnan.

[0099] This example is based on the HPLC determination of the contents of danshenin sodium, rosmarinic acid, shikonic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in purple salvia miltiorrhiza produced in Yunnan Chuxiong, including the following steps:

[0100] Step (1), preparation of reference substance solution:

[0101] Accurately weigh the appropriate amount of Danshensu sodium, rosmarinic acid, shikonic acid, salvianolic acid B, dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ, tanshinone ⅡA reference substance, and add methanol to prepare 1 mL of danshensu sodium containing 16.48 μg, Reference substance solution of 12.32 μg of rosmarinic acid, 22.72 μg of shikonic acid, 404 μg of salvianolic acid B, 6.384 μg of dihydrotanshinone I, 14.08 μg of cryptotanshinone, 6.656 μg of...

Embodiment 2

[0113] This example is aimed at the detection method of the active ingredients of wild purple salvia miltiorrhiza.

[0114] In this example, based on HPLC, the content of danshenin sodium, rosmarinic acid, shikonian acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in wild purple salvia miltiorrhiza is determined, including the following steps:

[0115] Step (1), preparation of reference substance solution:

[0116] Accurately weigh the appropriate amount of Danshensu sodium, rosmarinic acid, shikonic acid, salvianolic acid B, dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ, tanshinone ⅡA reference substance, and add methanol to prepare 4.120 μg of danshensu sodium per 1 mL, Reference substance solution of 3.080 μg of rosmarinic acid, 5.680 μg of shikonic acid, 101 μg of salvianolic acid B, 1.596 μg of dihydrotanshinone I, 3.520 μg of cryptotanshinone, 1.640 μg of tanshinone IIA and 4.200 μg of tanshinone IIA. Instantly.

...

Embodiment 3

[0129] This embodiment is aimed at the detection method of the active ingredients in the medicinal material of Danshen.

[0130] This example is based on the HPLC determination of the contents of danshenin sodium, rosmarinic acid, shikonic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in Danshen medicinal materials in various provinces, including the following steps:

[0131] Step (1), preparation of reference substance solution:

[0132] Precisely weigh the appropriate amount of Danshensu Sodium, Rosmarinic Acid, Shikoninic Acid, Salvianolic Acid B, Dihydrotanshinone Ⅰ, Cryptotanshinone, Tanshinone Ⅰ, Tanshinone ⅡA reference substance, add methanol to prepare 41.20 μg of Danshensu Sodium per 1 mL, A solution of 30.80 μg of rosmarinic acid, 56.80 μg of shikonic acid, 1010 μg of salvianolic acid B, 15.96 μg of dihydrotanshinone I, 35.20 μg of cryptotanshinone, 16.40 μg of tanshinone I, and 42.00 μg of tanshinone IIA was obtain...

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Abstract

The invention relates to a method for detecting active ingredients of salviae miltiorrhizae and products. The method comprises the following steps: preparation of a test solution, preparation of a reference solution and sample detection. In the method provided by the invention, index ingredients such as sodium danshensu, rosmarinic acid, alkannic acid, danshinolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone II contained in salviae miltiorrhizae and preparations thereof are simultaneously detected for the first time to realize more comprehensive evaluation and control of the quality of salviae miltiorrhizae and preparations thereofs. The detection time is shortened, the detection cost is reduced, the method is not only accurate, but also is simple and feasible. The method provided by the invention has good stability, good repeatability, high recovery rate and relatively high specificity.

Description

technical field [0001] The invention relates to a method for detecting effective components, in particular to a method for detecting effective components of salvia miltiorrhiza and its products, and belongs to the field of quality detection of Chinese medicinal materials and preparations thereof. Background technique [0002] Purple salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza, which is mainly produced in Yunnan, Shanxi, Hebei, Shandong, Shaanxi, Jiangsu, Jiangxi and Hunan. Purple salvia miltiorrhiza is bitter in taste, slightly pungent, and slightly cold in nature, and has the functions of nourishing blood and calming the nerves, promoting blood circulation and removing blood stasis, cooling blood and reducing swelling. It not only has the functions of promoting blood circulation and removing stasis, clearing away heat and detoxification, analgesia, and calming the nerves, but also dilates blood vessels and increases coronary blood flow. Clinicall...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 李俊管小军厉君
Owner YUNNAN CHUXIONG TIANLI PHARMA
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