A kind of mineralized keratin biomimetic material and preparation method thereof
A technology of bionic materials and keratin, which is applied in the field of biomedical materials, can solve the problems of unsatisfactory and stable promotion of DPSCs proliferation, and achieve the effect of promoting growth
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Embodiment 1
[0033] Example 1: Constructing a mineralized corneum bionic material
[0034] A method of preparing a mineralized keratin bionic material comprising the following steps:
[0035] 1) Adding a sterile simulated body fluid environment at a concentration of 100 mg / ml of a concentration of 100 mg / mL, and the mineralization treatment is separated, and the mixture is slightly dispersed every 12h, where it is sampled as a sample, sampling The mineralized corneum was placed in a 1.5 ml EP tube, and the centrifugation was centrifuged at room temperature for 15 min, discarded, collecting precipitation, resuspended with deionic water, 3 times, vacuum freeze, grinding into powder, respectively, respectively The mineralized corneum particles were obtained from the 1 to 6 weeks of mineralization and used for subsequent physical and chemical characterization experiments;
[0036] 2) Taking step 1) The resulting mineralized cormope particles of the resulting mineralization were resuspended int...
Embodiment 2
[0037] Example 2: Constructing a mineralized corneum bionic material
[0038] A method of preparing a mineralized keratin bionic material comprising the following steps:
[0039] 1) Take the namdone protein powder to add a sterile simulated body fluid environment at a concentration of 0.5 mg / ml, and then stand for 6 weeks, slightly stirred every 12h. Sampling at the end of mineralization, and put the mineral keratin In a 1.5 ml EP tube, 1 min was centrifuged at room temperature, discarded, collecting precipitation, resuspended with deionic water, washed 3 times, vacuum freeze drying, ground into powder to obtain mineral keratin particles;
[0040] 2) Taking step 1) The resulting mineralized corneum particles are resuspended into the 24-hole cell culture plate at a concentration of 500 mg / mL, and is placed in a 24-hole cell culture plate, and is placed in an oven at 20 ° C. 5H, Example 2 Mineralized corneum bionic material.
Embodiment 3
[0041] Example 3: Constructing mineralized keratin bionic materials
[0042] A method of preparing a mineralized keratin bionic material comprising the following steps:
[0043] 1) Adding a sterile simulated body fluid environment at a concentration of 500 mg / ml of a concentration of 500 mg / ml, and the mineralization treatment is 3 weeks, and the mixture is slightly dispersed every 12h. When the mineralization is ended, the mineral keratin is placed in In the 1.5 ml EP tube, 1000 rpm was centrifuged at room temperature for 30 min, discarded, collected, resuspended with deionic water, washed 3 times, vacuum freeze drying, ground into powder, to obtain mineral keratin particles;
[0044] 2) Take step 1) The resulting mineral keratin particles are resuspended in a 24-well cell culture plate at a concentration of 0.1 mg / ml, and is placed in an oven at 85 ° C after returning at the bottom of the 24-well cell culture plate. It was dried for 1 h to give Example 3 Mineralized corneu...
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