Method for regulating and controlling growth metabolic rule of indigenous microorganisms of oil pool
An endogenous microorganism and oil reservoir technology, applied in the field of microbial oil recovery, can solve the problems of no contribution to oil displacement effect, reduced water content, obvious oil displacement effect, etc., achieving targeted and operability, high input-output ratio, Controlling the effect of clear thinking
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Embodiment 1
[0029] JN in a block of Shengli Oilfield 12 The reservoir temperature is 70℃, the porosity is 32.5%, and the average permeability is 1500×10 -3 μm 2 , Crude oil viscosity 1256mPa·s, salinity 12368mg / L, geological reserves of 5.0×10 6 t, the comprehensive water content of the block before the test is 98.5%. Using the method of the present invention to implement microbial flooding to improve the oil recovery rate of the block, the specific steps are as follows:
[0030] (1) Sampling of test reservoirs
[0031] From the trial block JN 12 The obtained field samples are separated from oil and water to obtain the test block JN 12 5L of formation water sample.
[0032] (2) Determination of the growth and metabolism of endogenous microorganisms in oil reservoirs
[0033] First screen out the total bacterial concentration after activation is greater than 5.0×10 8 A / mL activator formula, the test results are shown in Table 1. The selected activator formula is whey 3.0g / L, urea 0.5g / L, and diamm...
Embodiment 2
[0059] GM in a block of Shengli Oilfield 21 The reservoir temperature is 75℃, the porosity is 31.5%, and the average permeability is 1200×10 -3 μm 2 , Crude oil viscosity is 2532mPa·s, salinity is 9860mg / L, geological reserve is 7.8×10 6 t, the comprehensive water content of the block before the test is 99.2%. Using the method of the present invention to implement microbial flooding to improve the oil recovery rate of the block, the specific steps are as follows:
[0060] (1) Sampling of test reservoirs
[0061] From the test block GM 21 The obtained field samples are separated from oil and water to obtain the test block GM 21 8L of formation water sample.
[0062] (2) Determination of the growth and metabolism of endogenous microorganisms in oil reservoirs
[0063] First screen out the total bacterial concentration after activation is greater than 5.0×10 8 A / mL activator formula, the test results are shown in Table 5. The selected activator formula is starch 5.0g / L, amino acid 0.4g / L...
Embodiment 3
[0091] JL in a block of Shengli Oilfield 5 The reservoir temperature is 85℃, the porosity is 33.1%, and the average permeability is 900×10 -3 μm 2 , Crude oil viscosity 3212mPa·s, salinity 9885mg / L, geological reserves of 9.2×10 6 t, the comprehensive water content of the block before the test is 98.7%. Using the method of the present invention to implement microbial flooding to improve the oil recovery rate of the block, the specific steps are as follows:
[0092] (1) Sampling of test reservoirs
[0093] From the trial block JL 5 The obtained on-site samples are separated from oil and water to obtain the test block JL 5 10L of formation water sample.
[0094] (2) Determination of the growth and metabolism of endogenous microorganisms in oil reservoirs
[0095] First screen out the total bacterial concentration after activation is greater than 5.0×10 8 A / mL activator formula, the test results are shown in Table 9. The selected activator formula is starch 1.0g / L, beef extract 0.4g / L, a...
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