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Method for comparison of virulence of goat mycoplasma goat pneumonia subspecies strains

A technology for mycoplasma and goats, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of complex test operation, high feeding requirements, high price, etc., achieve good repeatability, reduce workload, and low cost Effect

Active Publication Date: 2020-02-21
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the experimental animal goat has disadvantages such as relatively expensive price, high feeding requirements, complicated test operation, and heavy workload.

Method used

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  • Method for comparison of virulence of goat mycoplasma goat pneumonia subspecies strains

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Embodiment 1

[0013] The present invention is illustrated below in conjunction with examples.

[0014] The method for comparing the virulence of Mycoplasma capricosum goat pneumonia subspecies strain of the present invention, the steps are as follows:

[0015] (1) Preparation of SPF chicken embryos: 7-8 days old healthy SPF chicken embryos were prepared. SPF eggs were purchased from Shandong Haotai Experimental Animal Breeding Co., Ltd. and incubated in an incubator (temperature 37.0-37.5°C, humidity 50%). Pick chicken embryos with good development, clear blood vessels, and vigorous vitality for vaccination, and discard non-fertilized eggs, dead embryos, and weak embryos.

[0016] (2) Mycoplasma capricoides subsp. goat pneumonia bacterial liquid preparation: resuscitate Mycoplasma goati subsp. goat pneumoniae F38 strain (NCTC, British Type Culture Collection Center, No. NCTC10192), M1801 strain (preserved by Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences), a...

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Abstract

The invention discloses a method for simply comparison of the virulence of goat mycoplasma goat pneumonia subspecies strains. The method comprises the steps: inoculating the resurgent goat mycoplasmagoat pneumonia subspecies strains of equal concentration to chicken embryos, then putting the inoculated chicken embryos in a thermostat box for cultivation, detecting the status and death situationsof the chicken embryos periodically, counting the fatality of the chicken embryos inoculated with the strains, and adopting the fatality for comparison and judgment. The method has the advantages of simple operation, low cost, no need for complicated conditions of animal breeding, easy availability of experimental animals, a short consumed time, good repeatability and the like, and the workload ofanimal experiments is reduced greatly, so that the defects of high price of experimental animal goats, large individual differences, high breeding requirements, complex experimental operation, a longexperiment cycle and high workload in the prior art are overcome.

Description

technical field [0001] The invention relates to a method for comparing certain properties of different strains of the same microorganism, in particular to a method for simply comparing the virulence of Mycoplasma capricosum subsp. goat pneumoniae strains. Background technique [0002] Goat contagious pleuropneumonia (CCPP) is an animal disease that seriously endangers the sheep industry. At present, the disease is mainly prevalent in Asia and Africa, and it is an OIE legally notifiable disease. The pathogen of the disease is Mycoplasma capricosum subspecies goat pneumonia, which mainly infects goats and wild goats, causing clinical symptoms such as fever, cough and dyspnea. To study and prepare the Mycoplasma capricum subspecies capricum pneumoniae vaccine, it is necessary to compare the virulence of different bacterial strains in order to obtain the best result of immune effect. It is also often necessary to determine its virulence in the pathogenic mechanism and virulence...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 陈胜利储岳峰郝华芳颜新敏刘永生毛婷
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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