Cancer suppressor gene and application thereof
A technology of gene and gene coding, applied in the field of tumor suppressor gene and its application, can solve the problem of less research on the application value of nasopharyngeal carcinoma, achieve the effect of inhibiting growth and invasion, and improving the expression of VILL gene
Active Publication Date: 2020-02-21
SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV +1
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Problems solved by technology
The current research results show that free DNA (cell free DNA, cfDNA) exists in the peripheral blood of various malignant tumors, such as lung cancer, liver cancer, esophageal cancer, breast cancer, colorectal cancer, etc., and is positively correlated with the occurrence and development of tumors. There are few studies on the application value of nasopharyngeal carcinoma
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[0037] In order to demonstrate the technical solutions, objectives and advantages of the present invention more concisely and clearly, the present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings.
[0038] 1. Materials and methods
[0039] 1.1 Main materials
[0040] The peripheral blood of 4 normal people and 6 patients with nasopharyngeal carcinoma, and the tissue of 10 human nasopharyngeal carcinomas were provided by Nanfang Hospital (cfDNA methylation detection was performed by Shenzhen Aise Gene Technology Co., Ltd.; immunohistochemistry was handed over by Nanfang Hospital Guangzhou Huayin Medical Laboratory Center, Medical University). Human kidney embryonic cell 293T was from the Cancer Institute of Southern Medical University; human nasopharyngeal carcinoma cell line HONE1 EBV was provided by Professor S.-W.Tsao of the University of Hong Kong; pTSB02-EGFP-FLAG-VILL and pTSB02-EGFP-FLAG were purchase...
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The invention discloses a diagnosis and treatment marker for nasopharyngeal carcinoma. The marker is VILL, experiments show that the expression of the VILL and a protein thereof is significantly reduced in nasopharyngeal carcinoma tissue and suggest that the VILL can be used in the diagnosis or treatment of the nasopharyngeal carcinoma. Further, in-vitro cell experiments confirm that the VILL is related to the proliferation, apoptosis, migration, and invasion of nasopharyngeal carcinoma cells and suggest that the VILL can be used as a drug target in the clinical treatment of the nasopharyngealcarcinoma. The invention also discloses the protein EIF3J interacting with a VILL gene. Experiments find that the EIF3J is positively correlated with the VILL and suggest that the expression level ofthe VILL gene can be increased through the EIF3J, so that the purpose of treating the nasopharyngeal carcinoma or nasopharyngeal carcinoma metastasis is achieved, and a novel treatment idea is provided.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a tumor suppressor gene related to nasopharyngeal carcinoma and its application. Background technique [0002] Nasopharyngeal carcinoma originates from the mucosa of the top and side walls of the nasopharyngeal cavity, and its incidence rate has become the first among head and neck tumors in my country. The incidence of nasopharyngeal carcinoma is high in southern my country, and its incidence decreases northward, and the incidence rate of men is higher than that of women. Interestingly, some studies have shown that Guangdong and Guangxi have a higher incidence of nasopharyngeal cancer because Cantonese is widely spoken. It is worth noting that the incidence of nasopharyngeal cancer remains high even after the southern population immigrated to other regions. This suggests that family inheritance, living habits, susceptibility genes and geographical distribution are clos...
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IPC IPC(8): C12Q1/6886G01N33/574G01N33/68A61K38/19A61P35/00
CPCC12Q1/6886G01N33/57407G01N33/57488G01N33/68A61K38/19A61P35/00C12Q2600/158G01N2333/4704
Inventor 黄仲曦申维玺温悦婷章建平潘星华
Owner SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV