A method for improving the degradation rate of diethylstilbestrol by laccase

A technology of diethylstilbestrol and degradation rate, applied in chemical instruments and methods, biochemical equipment and methods, enzymes, etc., can solve problems such as decreased laccase processing ability

Active Publication Date: 2021-12-07
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when it is used for DES to degrade sewage, it generally takes about 6 hours to complete the degradation. However, in sewage with DES concentration higher than 50mg / L, the treatment capacity of laccase decreases, and the highest degradation rate is only 70%. about
Therefore, the degradation efficiency and degradation rate of laccase for DES sewage need to be further improved.

Method used

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  • A method for improving the degradation rate of diethylstilbestrol by laccase
  • A method for improving the degradation rate of diethylstilbestrol by laccase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Standard Preparation: diethylstilbestrol dissolved in methanol to form a 2.5g / L diethylstilbestrol mother liquor, the mother liquor was diluted diethylstilbestrol are released to a concentration 1,5,10,20,30,50mg / L, 100mg / L standard solution, diethylstilbestrol standard curve prepared using HPLC peak area detected.

[0032] The HPLC detection conditions are as follows:

[0033] Column: ZORBAX SB-C18 (150mm × 4.6mm × 5μm); detection conditions: injection volume 10μL, an equal volume of acetonitrile and water at the flow gradient 10min, the pump flow rate was 1.0mL / min, column temperature : 30 ℃, an ultraviolet detector at a wavelength of 240nm where the peak, the peak area value recording.

Embodiment 2

[0035] pH (1) using sodium acetate and acetic acid is formulated as a 0.01mol / L acetic acid 5.5 - sodium acetate buffer solution;

[0036] (2) Step (1) Preparation of acetic acid - sodium acetate buffer solution prepared at a concentration of 1mg / mL laccase mother liquor and the concentration of 0.01mol / L of ABTS solution, wherein the laccase is Coriolus versicolor produced, activity of 0.94U / mg;

[0037] (3) take 0.5mL laccase liquor, 0mL ABTS solution was added 0.4mL and 10mL DES mother liquor of the reaction system, the remaining solution is filled by the buffer, placed in a reaction 55 ℃ shaking incubator, set the speed 120r / min, the reaction using ethyl acetate solution was extracted twice after 0.5h, the organic phase was extracted rotary evaporated to dryness to do, using constant volume of methanol by vacuum filtration membrane sample, measure the concentration of the sample after the reaction by HPLC.

Embodiment 3

[0039] In Example 2, except that the step (3) Laccase 0.5mL mother liquor and the mother liquor and DES 0.4mL 0.1mL ABTS was added 10mL reaction system, filled by the remaining buffer solution.

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Abstract

The invention relates to a method for improving the degradation rate of laccase-degrading diethylstilbestrol, specifically, ABTS and laccase solution are used to form a laccase-ABTS medium solution, and then mixed with the diethylstilbestrol solution in the reaction system, and put into a shaking incubator to react for a period of time After a period of time, ethyl acetate is used to extract diethylstilbestrol to terminate the reaction to achieve the degradation of diethylstilbestrol. The present invention increases the degradation rate of diethylstilbestrol by laccase from the original 44.5% to 99.9% by preparing a laccase-ABTS medium solution and using it for the degradation of diethylstilbestrol. This is beneficial to the treatment of diethylstilbestrol sewage, and has practical application value in the field of sewage treatment.

Description

Technical field [0001] The present invention relates to the field of wastewater treatment technologies, particularly to a method for improving the degradation diethylstilbestrol laccase degradation rate. Background technique [0002] Diethylstilbestrol (Diethylstilbestrol, DES) is a synthetic non-steroidal estrogens or estrogen isobutyl, having stronger than the estrogenic activity of estradiol. From the beginning of 1938, it had been used in oral contraceptives and growth promoters in animal husbandry. After the study was found to be an endocrine disruptors, DES exposure have a strong negative impact on humans and animals, and even exposure to this synthetic estrogen cancer risk has been brought to the world. Had this, the US National Cancer Institute study reported that the use of DES during pregnancy, reproductive tract abnormalities, increasing the relative risk of infertility, pregnancy complications, cervical intraepithelial neoplasia (CIN) and clear cell adenocarcinoma. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N9/96C12N9/02C02F101/34
CPCC02F3/342C02F2101/345C12N9/0061C12N9/96C12Y110/03002
Inventor 刘红艳谢世伟林晓莲李海云李子院张会香单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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