45 results about "Dimethylstilbestrol" patented technology

The invention relates to the technical fields of separation and analysis and particularly discloses a preparation method and application of a magnetic diethylstilbestrol molecularly-imprinted polymer based on an organic-inorganic hybridizing technology. The preparation method comprises the steps of preparing ferroferric oxide particles by using a coprecipitation method; preparing silanized ferroferric oxide by using a stober method; preparing an organic and inorganic hybrid magnetic diethylstilbestrol molecularly-imprinted polymer by using a bulk polymerization method; and grinding the polymer, sieving, adding an eluent to extract and elute, and then, drying to obtain the magnetic diethylstilbestrol molecularly-imprinted polymer. A target product, namely diethylstilbestrol, can be rapidly combined on the surface of the magnetic diethylstilbestrol molecularly-imprinted polymer synthesized by using the preparation method and can be rapidly separated from a reaction system under the action of an external magnetic field, so that the aim of efficiently separating a trace amount of diethylstilbestrol from a complex matrix at high specificity is achieved, and the sample pretreatment efficiency is greatly increased.

The invention provides cerrena unicolor derived laccase and a gene and application thereof, and belongs to the field of biotechnology. The amino acid sequence of the cerrena unicolor derived LacB laccase provided by the invention is as shown in SEQID NO.3, the overall-length sequence of the gene of the cerrena unicolor derived LacB laccase is as shown in SEQID NO.1, and the cDNA sequence is as shown in SEQID NO.2. The invention further discloses a fermentation production method of the LacB laccase. The LacB laccase is produced through fermentation of the cerrena unicolor, after the sixth day of fermentation culture, the laccase yield can reach 652 U/mL, and the fermentation production method has the advantages of being high in yield and short in enzyme production cycle. The LacB laccase has significant effects in environmental hormone degradation, and the degradation rate on diethylstilbestrol, hexestrol, dienestrol, beta-estradiol, bisphenol a and hydroprednisone is 98% or above. TheLacB laccase provides a new way for environmental pollutant government, and has good economic benefits and social benefits.

The invention discloses a multifunctional nano-enzyme. The multifunctional nano-enzyme is a nano-material formed by terbium ions, copper ions, cerium oxide and pyridine dicarboxylic acid through solvothermal reaction. The multifunctional nano-enzyme has the catalysis function and the luminescence indication function of peroxidase and laccase, and can catalytically degrade and emit light to displaydiethylstilbestrol. The invention also discloses a preparation method of the multifunctional nano-enzyme and application of the multifunctional nano-enzyme in degradation and determination of the diethylstilbestrol. The multifunctional nano-enzyme has high catalytic activity and stability, is low in cost and can be applied to removal and monitoring of the diethylstilbestrol in the environment.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

The invention discloses an electrochemical luminescence method for detecting diethylstilbestrol, and particularly belongs to the field of electrochemical luminescence detection. The operation processcomprises the steps of: (1) preparing a composite material of perylenetetracarboxylic acid (PTCA) immobilized aminated Ti-based MOFs (NH2-MIL-125); (2) preparing an electrochemical luminescence aptamer (apy) sensor; and (3) detecting diethylstilbestrol by using an electrochemical luminescence method, wherein an pt/PTCA/NH2-MIL-125 modified glassy carbon electrode is used as an operating electrode,an Ag/AgCl electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and a traditional three-electrode system is formed. The detection range of the electrochemical luminescence method is 1.0*10<-15> mol/L to 1.0*10<-6 > mol/L, and the lowest detection limit is 2.8*10<-16> mol/L. The electrochemical luminescence method for detecting diethylstilbestrol is low in cost, high in sensitivity, strong in specificity and simple to operate.

The invention discloses a method for degrading diethylstilbestrol by using a Trametes versicolor crude enzyme solution, and belongs to the technical field of environmental remediation, wherein the method comprises three steps of preparation of a Trametes versicolor fermentation broth, preparation of a Trametes versicolor crude enzyme liquid, and utilizing. In a process for degrading diethylstilbestrol by using a Trametes versicolor crude enzyme solution, 100-150 [mu]L of a 2.5 g/L DES-methanol solution and 1-2 mL of a Trametes versicolor extracellular crude enzyme solution are added, a 7.85-8.9 mL of an acetic acid buffer solution is added, and a reaction is performed for 3-4 h to complete the degradation of diethylstilbestrol. According to the invention, the method adopts a biological enzyme catalysis process, and has characteristics of high diethylstilbestrol removal rate, safety, no pollution and the like.

The invention discloses a method for improving the degradation rate of laccase for degrading diethylstilbestrol by using acetosyringone, and an application thereof, and belongs to the technical fieldof environmental pollution treatment. The method comprises the following steps: forming an acetosyringone-laccase medium buffer solution from acetosyringone and laccase, mixing the acetosyringone-laccase medium buffer solution with a diethylstilbestrol solution, performing a reaction in a shaking incubator, and quenching the reaction by using ethyl acetate to realize the degradation of the diethylstilbestrol. The acetosyringone is used as a redox medium for electron shuttle between laccase and a substrate, so that the steric hindrance of laccase is overcome, thereby the laccase can be well combined with the diethylstilbestrol, and the degradation efficiency of the laccase is remarkably improved. The method is simple and efficient to operate, and has extremely high application values in environmental pollution treatment.

The invention discloses a method for rapid catalytic degradation of diethylstilbestrol, and belongs to the technical field of environmental pollution treatment. The method comprises the following steps: dissolving diethylstilbestrol in methanol to form diethylstilbestrol mother liquor; dissolving laccase in water to form laccase mother liquor; diluting the diethylstilbestrol mother liquor into diethylstilbestrol standard solutions with different concentrations; mixing the diethylstilbestrol standard solution with the laccase mother solution, adding a buffer solution, extracting diethylstilbestrol with ethyl acetate, and terminating the reaction, wherein the reaction conditions are that the reaction temperature is 25-75 DEG C, the reaction pH is 3-8, and the degradation rate of a diethylstilbestrol solution with an initial concentration of 50 mg/L reaches 93.1% after the reaction is conducted for 6 h, the reaction is rapid, and secondary pollution is avoided.

The invention discloses an application of vanillin as a redox medium in increasing the diethylstilbestrol degradation rate of laccase. The concentration of vanillin is 0.01-1 mmol/L. The invention also discloses a method for increasing the diethylstilbestrol degradation rate of laccase by taking vanillin as the redox medium. The method comprises the following steps: 1, dissolving the laccase intoan acetic acid-sodium acetate buffer solution to prepare a laccase mother liquor, dissolving diethylstilbestrol in methanol to prepare a diethylstilbestrol solution, and dissolving vanillin in the acetic acid-sodium acetate buffer solution to prepare a vanillin solution; 2, mixing the prepared laccase mother liquor with the vanillin solution to obtain a laccase-vanillin solution, mixing the laccase-vanillin solution with the prepared diethylstilbestrol solution in a reaction system, putting the reaction system into a shaking incubator, and carrying out a reaction; and 3, extracting with ethylacetate extraction after the reaction is performed for half an hour in order to terminate the diethylstilbestrol reaction and complete degradation. The method adopting the vanillin as the reducing medium greatly increases the DES degradation rate of laccase, and reduces the degradation cost.

The invention provides a diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, a preparation method and application, and belongs to the technical field of analytical chemistry. A rabbit is fed with feedstuff containing diethylstilbestrol, diethylstilbestrol and hexestrol, so that tissues in the body of the rabbit contains the diethylstilbestrol, the diethylstilbestrol and the hexestrol, after administration is finished, the rabbit is killed, and muscles of the rabbit are taken, are homogenized and homogenized, are processed into meat paste, are freeze-dried under vacuum, and are sieved, bottled and packed to obtain a rabbit meat standard sample containing the diethylstilbestrol, the diethylstilbestrol and the hexestrol. By the method, the problems that a target object and matrix combination situation is not completely consistent to a real detecting sample, so that treatment results of extracting, purifying and the like are quite different from daily analysis samples are solved. The standard sample is a material standard sample, is uniform and stable, does not contain a preservative or an antioxidant, is easy to store, and is used for proficiency testing of a non-steroidal anabolic hormones drug residue testing item, verification of a detecting method, calibration of a testing instrument, and quality control and assessment of a testing result.

The invention provides a diethylstilbestrol (DES) mimic antigenic epitope peptide, and a preparation method and an application thereof. Firstly, based on a phage display peptide library technology, ananti-DES monoclonal antibody is used as a target, and a DES antigen mimic epitope having the sequence of YVYPQQK, YVYPPGP or YVYPQSR is selected from a phage random display heptapeptide library. Centering on immune binding characteristics of the antigen mimic epitope, a specific detection method is designed based on enzyme-linked immunosorbent assay, colloidal gold immunochromatography and fluorescent microsphere immunochromatography. The antigenic mimic epitope has similar immunoreactivity characteristics to those of natural DES molecules, can replace an expensive DES standard substance having teratogenic and carcinogenic effects to be used in immunological detection of DES; the detection results are accurate and the sensitivity is high. The harm to human body health in the DES detectionprocess is effectively reduced, and meanwhile, the cost is saved.

The invention discloses a photo-electrochemical diethylstilbestrol biosensor constructed based on a boron-doped poly-metallic oxide as well as preparation and application thereof. In a preparation method, firstly, a two-dimension nano material g-C3N4 is modified on ITO conductive glass by adopting an electro-deposition method; then, an iron cobalt hydroxide is subjected to in-situ growth by adopting a hydrothermal method, and further, a working electrode modified by a g-C3N4-loaded boron-doped iron cobalt oxide Bi@NixFe1-xO3/g-C3N4 is obtained; finally, a diethylstilbestrol antibody is loaded by utilizing good biocompatibility and large specific surface area of the material. During detection, as the boron-doped iron cobalt oxide Bi@NixFe1-xO3/g-C3N4 can catalyze L-ascorbic acid-2-trisodium phosphate AAP, L-ascorbic acid AA is generated in situ, and further, an electron donor is provided for photoelectric detection; by utilizing the effect of specific quantitative binding of the antibody and an antigen on electronic transmission capacity, the photocurrent strength is correspondingly reduced, and the construction of a photoelectric sensor for detecting diethylstilbestrol by adopting an unmarked photo-electrochemical method is finally realized.

The invention discloses a method for determining diethylstilbestrol by adopting up-conversion luminescence immunochromatography. The method is characterized in that according to the research, a solvothermal method is adopted to synthesize up-conversion nanoparticles (UCNPs)-NaYF4: Yb, Er, surface functionalization modification is carried out, the nanoparticles are coupled with a diethylstilbestrol(DES) monoclonal antibody to prepare a fluorescent probe, a nitrocellulose film is respectively sprayed on a bovine serum albumin-diethylstilbestrol (BSA-DES) conjugate and a goat anti-mouse secondary antibody to form a test strip detection line (T line) and a quality control line (C line), and an up-conversion luminescence immunochromatographic test strip is established for rapid quantitative detection; a method of DES is adopted; the experimental result shows that the DES linear quantitative detection range of the test strip is 25 to 10000 ng/mL (Y is equal to 0.43927 x-0.57647, and R2 is equal to 0.996), the detection limit is 20.84 ng/mL, the detection time of a single sample is 15 min, intra-batch and inter-batch variable coefficients are both smaller than 10%, the specificity recognition capacity is high, an average value of RSD detection values is about 15% after the kit is stored at 37 DEG C for 7 days; standard recovery experiments show that the average recovery rate is 90.1%-115.2%, relative standard deviation is smaller than 5%, and the method has good consistency with high performance liquid chromatography.

The invention relates to a preparing method of a qi-stagnancy and blood stasis uterus myoma model. Unpredictable stimulation is given while diethylstilbestrol liquid medicine and progesterone injection are given. The uterus myoma model can be established effectively, and the preparing method is safe, capable of being used for screening related drugs and good in application prospects.