46 results about "Dimethylstilbestrol" patented technology

The invention discloses a preparation method of a photoelectrochemical diethylstilbestrol sensor and belongs to the technical field of novel nano functional materials and biosensors. Firstly, a novel two-dimensional nano photoelectric material, namely a copper-doped nano photoelectric material, specifically a two-dimensional nano composite Cu-TiO2 / MoS2 obtained through in-situ compounding of molybdenum disulfide on a copper-doped titanium dioxide nano cube is prepared, and an diethylstilbestrol antibody is loaded and alkaline phosphatase is fixed to the material by the application of good biocompatibility and a large specific surface area of the material. During detection, due to the fact that alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate AAP to generate L-ascorbic acid AA in situ and then provide an electron donor for photoelectric detection, by the application of the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the photoelectric current intensity is lowered accordingly, and finally the photoelectric sensor for detecting diethylstilbestrol through an unmarked photoelectrochemical method is constructed.

The invention discloses a method for preparing a diethylstilbestrol molecularly imprinted electrochemical sensor based on electropolymerization of a p-aminothiophenol film. The method comprises sequential steps such as glassy carbon electrode pretreatment, electrode modification by multi-wall carbon nanotubes, electrode modification by nano gold, functional monomer self-assembly, template molecular pre-assembly, electropolymerization of the p-aminothiophenol film, and template molecule elution, and an organic phase as a polyelectrolyte solvent improves the number and selectivity of binding sites in a polymer film, and is beneficial to formation of a stable host-guest compound from a template molecule and a function monomer, so that the template molecule can maintain a good rigid structurein a polymer network structure and form a cavity with a memory effect. The preparation method is simple and easy to control, and the sensor has strong selectivity and stability, high sensitivity and low cost. The sensor is suitable for analysis and detection of diethylstilbestrol residues in animal-derived foods, feeds and other samples.

The invention relates to a multifunctional special feed for Tadpole of Rants chensinensis. The technical proposal adopted is that the feed consists of main materials and auxiliary materials; wherein, every ton of the main materials consists of 200 to 400kg of rice bran, 100 to 200kg of corn meal, 100 to 200kg of fish meal, 100 to 300kg of bean pulp powder, 100 to 200kg of meat meal, 50 to 100kg of wheat-middling and 20 to 70kg of wheat bran; every ton of the main materiala is added into the following auxiliary materials by weight: 200 to 800g of microbial inoculum agent, 200 to 500g of aquaculture vitamin complex, 200 to 500g of aquaculture compound mineral, 200 to 500g of antioxidant, 20 to 100g of inactivated aeromonas hydrophila vaccine and 20 to 50g of diethylstilbestrol. The preparation method is that firstly, the main materials are smashed, stirred; and then the auxiliary materials are added into the main materials and are stirred evenly, regulated in steam, granulated and cooled. The feed has the functions of sex induction and disease prevention for the Tadpole of Rants chensinensis.

The invention discloses a diethylstilbestrol degradation strain and an application thereof in sewage and animal manure treatment. Serratieae (Serratia sp.) S is preserved at China General Microbiological Culture Collection Center on August 26, 2014, wherein the strain preservation number is CGMCC 1.12941. The invention also discloses an application of the serratieae S and a fungicide of the serratieae S in degradation of estrogen, especially degradation of diethylstilbestrol. The serratieae S is obtained in a screening manner, the degradation rate on DES (diethylstilbestrol) of which the initial concentration is 50mg L<-1> can reach over 80% after an immobilized bacteria agent which is prepared from the strain is subjected to shake-flask culture in a laboratory for 7d, the immobilized bacteria agent is directly applied to DES-polluted water and animal manure, and the DES residue within a short period of time is reduced by over 90%, so that the problem of hazards of diethylstilbestrol DES to the water environment, animal manure and human health is solved.

The invention relates to the technical fields of separation and analysis and particularly discloses a preparation method and application of a magnetic diethylstilbestrol molecularly-imprinted polymer based on an organic-inorganic hybridizing technology. The preparation method comprises the steps of preparing ferroferric oxide particles by using a coprecipitation method; preparing silanized ferroferric oxide by using a stober method; preparing an organic and inorganic hybrid magnetic diethylstilbestrol molecularly-imprinted polymer by using a bulk polymerization method; and grinding the polymer, sieving, adding an eluent to extract and elute, and then, drying to obtain the magnetic diethylstilbestrol molecularly-imprinted polymer. A target product, namely diethylstilbestrol, can be rapidly combined on the surface of the magnetic diethylstilbestrol molecularly-imprinted polymer synthesized by using the preparation method and can be rapidly separated from a reaction system under the action of an external magnetic field, so that the aim of efficiently separating a trace amount of diethylstilbestrol from a complex matrix at high specificity is achieved, and the sample pretreatment efficiency is greatly increased.

The invention provides cerrena unicolor derived laccase and a gene and application thereof, and belongs to the field of biotechnology. The amino acid sequence of the cerrena unicolor derived LacB laccase provided by the invention is as shown in SEQID NO.3, the overall-length sequence of the gene of the cerrena unicolor derived LacB laccase is as shown in SEQID NO.1, and the cDNA sequence is as shown in SEQID NO.2. The invention further discloses a fermentation production method of the LacB laccase. The LacB laccase is produced through fermentation of the cerrena unicolor, after the sixth day of fermentation culture, the laccase yield can reach 652 U / mL, and the fermentation production method has the advantages of being high in yield and short in enzyme production cycle. The LacB laccase has significant effects in environmental hormone degradation, and the degradation rate on diethylstilbestrol, hexestrol, dienestrol, beta-estradiol, bisphenol a and hydroprednisone is 98% or above. TheLacB laccase provides a new way for environmental pollutant government, and has good economic benefits and social benefits.

The invention discloses a multifunctional nano-enzyme. The multifunctional nano-enzyme is a nano-material formed by terbium ions, copper ions, cerium oxide and pyridine dicarboxylic acid through solvothermal reaction. The multifunctional nano-enzyme has the catalysis function and the luminescence indication function of peroxidase and laccase, and can catalytically degrade and emit light to displaydiethylstilbestrol. The invention also discloses a preparation method of the multifunctional nano-enzyme and application of the multifunctional nano-enzyme in degradation and determination of the diethylstilbestrol. The multifunctional nano-enzyme has high catalytic activity and stability, is low in cost and can be applied to removal and monitoring of the diethylstilbestrol in the environment.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

The invention discloses an application of a dienestrol antibody in an immune electrochemical detection method of simultaneous detection of three female hormone analogous compounds, namely, diethylstilbestrol, dienestrol and hexestrol. The detection method includes following steps: (1) preparing an electrochemical sensor deposited by nano gold and modified by a graphene / strol / chitosan composition; (2) with K3[Fe(CN)6] as a probe, performing the simultaneous detection of the three strol on the basis of a cross immunoreaction of the dienestrol antibody to the three strol. In the method, a detection limit is 0.2 ng / mL and a linearity range is 1-4000 ng / mL of the diethylstilbestrol; the detection limit is 0.05 ng / mL and the linearity range is 10-4000 ng / mL of the dienestrol; and the detection limit is 0.5 ng / mL and the linearity range is 100-4000 ng / mL of the hexestrol.

The invention discloses an electrochemical luminescence method for detecting diethylstilbestrol, and particularly belongs to the field of electrochemical luminescence detection. The operation processcomprises the steps of: (1) preparing a composite material of perylenetetracarboxylic acid (PTCA) immobilized aminated Ti-based MOFs (NH2-MIL-125); (2) preparing an electrochemical luminescence aptamer (apy) sensor; and (3) detecting diethylstilbestrol by using an electrochemical luminescence method, wherein an pt / PTCA / NH2-MIL-125 modified glassy carbon electrode is used as an operating electrode,an Ag / AgCl electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and a traditional three-electrode system is formed. The detection range of the electrochemical luminescence method is 1.0*10<-15> mol / L to 1.0*10<-6 > mol / L, and the lowest detection limit is 2.8*10<-16> mol / L. The electrochemical luminescence method for detecting diethylstilbestrol is low in cost, high in sensitivity, strong in specificity and simple to operate.

The invention discloses a method for degrading diethylstilbestrol by using a Trametes versicolor crude enzyme solution, and belongs to the technical field of environmental remediation, wherein the method comprises three steps of preparation of a Trametes versicolor fermentation broth, preparation of a Trametes versicolor crude enzyme liquid, and utilizing. In a process for degrading diethylstilbestrol by using a Trametes versicolor crude enzyme solution, 100-150 [mu]L of a 2.5 g / L DES-methanol solution and 1-2 mL of a Trametes versicolor extracellular crude enzyme solution are added, a 7.85-8.9 mL of an acetic acid buffer solution is added, and a reaction is performed for 3-4 h to complete the degradation of diethylstilbestrol. According to the invention, the method adopts a biological enzyme catalysis process, and has characteristics of high diethylstilbestrol removal rate, safety, no pollution and the like.

The invention discloses a method for improving the degradation rate of laccase for degrading diethylstilbestrol by using acetosyringone, and an application thereof, and belongs to the technical fieldof environmental pollution treatment. The method comprises the following steps: forming an acetosyringone-laccase medium buffer solution from acetosyringone and laccase, mixing the acetosyringone-laccase medium buffer solution with a diethylstilbestrol solution, performing a reaction in a shaking incubator, and quenching the reaction by using ethyl acetate to realize the degradation of the diethylstilbestrol. The acetosyringone is used as a redox medium for electron shuttle between laccase and a substrate, so that the steric hindrance of laccase is overcome, thereby the laccase can be well combined with the diethylstilbestrol, and the degradation efficiency of the laccase is remarkably improved. The method is simple and efficient to operate, and has extremely high application values in environmental pollution treatment.

The invention discloses a method for rapid catalytic degradation of diethylstilbestrol, and belongs to the technical field of environmental pollution treatment. The method comprises the following steps: dissolving diethylstilbestrol in methanol to form diethylstilbestrol mother liquor; dissolving laccase in water to form laccase mother liquor; diluting the diethylstilbestrol mother liquor into diethylstilbestrol standard solutions with different concentrations; mixing the diethylstilbestrol standard solution with the laccase mother solution, adding a buffer solution, extracting diethylstilbestrol with ethyl acetate, and terminating the reaction, wherein the reaction conditions are that the reaction temperature is 25-75 DEG C, the reaction pH is 3-8, and the degradation rate of a diethylstilbestrol solution with an initial concentration of 50 mg / L reaches 93.1% after the reaction is conducted for 6 h, the reaction is rapid, and secondary pollution is avoided.

The invention belongs to the field of food and environment safety detection, and particularly relates to a preparation method and application of an up-conversion luminescence flexible biosensor for diethylstilbestrol detection. The preparation method comprises the following steps: step 1, preparing an oleic acid coated up-conversion nano material; step 2, preparing an aminated up-conversion nano material; step 3, preparing a flexible up-conversion luminescence sensor; and step 4, preparing a bio-functionalized flexible up-conversion luminescence sensor. The sensor is used for detecting diethylstilbestrol, and detection of diethylstilbestrol content in food and environmental samples is realized by establishing a diethylstilbestrol content detection standard curve; through preparation of the up-conversion luminescence flexible biosensor and a detection method of diethylstilbestrol, rapid and economical detection of diethylstilbestrol in food and environmental samples is realized, and the up-conversion luminescence flexible biosensor has a wide concentration detection range and a low detection limit; meanwhile, the sensor has a renewable function and has a good practical prospect.

The invention relates to a diethylstilbestrol single-chain antibody screening method and a purpose of a diethylstilbestrol single-chain antibody, and belongs to the technical field of biology. The single-chain antibody is screened from a ribosome showing mouse source natural antibody base and is subjected to soluble expression. The invention further relates to a diethylstilbestrol antibody screening method with a ribosome showing technology. The diethylstilbestrol single-chain antibody screened through the method can be used for pollution detection, development of immune quick test kits used for quick test of diethylstilbestrol residual and treatment of relevant diseases, and a foundation is laid for future detection and research on the diethylstilbestrol single-chain antibody.

The invention discloses a diethylstilbestrol artificial antigen and an antibody prepared from the antigen. The structural formula of a diethylstilbestrol half antigen is shown in a formula I. The original stereochemical structure of a DES molecule is remained, and production of a specific antibody is also facilitated. The diethylstilbestrol artificial antigen is a conjugate of a compound shown in the formula I and carrier protein. The invention also provides an antibody by taking the diethylstilbestrol artificial antigen as an immunogen and a kit for detecting diethylstilbestrol. The kit comprises the diethylstilbestrol half antigen and / or the diethylstilbestrol artificial antigen and / or antibody. The formula (I) is described in the specification.

The invention discloses an application of vanillin as a redox medium in increasing the diethylstilbestrol degradation rate of laccase. The concentration of vanillin is 0.01-1 mmol / L. The invention also discloses a method for increasing the diethylstilbestrol degradation rate of laccase by taking vanillin as the redox medium. The method comprises the following steps: 1, dissolving the laccase intoan acetic acid-sodium acetate buffer solution to prepare a laccase mother liquor, dissolving diethylstilbestrol in methanol to prepare a diethylstilbestrol solution, and dissolving vanillin in the acetic acid-sodium acetate buffer solution to prepare a vanillin solution; 2, mixing the prepared laccase mother liquor with the vanillin solution to obtain a laccase-vanillin solution, mixing the laccase-vanillin solution with the prepared diethylstilbestrol solution in a reaction system, putting the reaction system into a shaking incubator, and carrying out a reaction; and 3, extracting with ethylacetate extraction after the reaction is performed for half an hour in order to terminate the diethylstilbestrol reaction and complete degradation. The method adopting the vanillin as the reducing medium greatly increases the DES degradation rate of laccase, and reduces the degradation cost.

The invention relates to a preparation method and application of a photoelectrochemical diethylstilbestrol sensor based on a zinc and molybdenum co-doped bismuth vanadate array. The preparation methodspecifically comprises the following steps: taking the zinc and molybdenum co-doped bismuth vanadate array as a substrate material and electrically depositing the zinc and molybdenum co-doped bismuthvanadate array on indium tin oxide conductive glass; the wormlike porous structure of the zinc and molybdenum co-doped bismuth vanadate has a large surface area, so that light capture and biomoleculeloading can be increased; additional electrons can be added due to doping of zinc and molybdenum elements, so the separation efficiency of photo-induced electron holes is promoted; the prepared ferromanganese cyanide compound is a metal inorganic framework material with a catalytic function on hydrogen peroxide, and is used as a competitive marker; based on this, hydrogen peroxide is used as an electron donor in the system, a photoelectrochemical sensor based on a weakened signal competition strategy is constructed, sensitive detection of diethylstilbestrol is realized, and the method is of great significance to early diagnosis and monitoring of autoimmune diseases.

The invention provides a diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, a preparation method and application, and belongs to the technical field of analytical chemistry. A rabbit is fed with feedstuff containing diethylstilbestrol, diethylstilbestrol and hexestrol, so that tissues in the body of the rabbit contains the diethylstilbestrol, the diethylstilbestrol and the hexestrol, after administration is finished, the rabbit is killed, and muscles of the rabbit are taken, are homogenized and homogenized, are processed into meat paste, are freeze-dried under vacuum, and are sieved, bottled and packed to obtain a rabbit meat standard sample containing the diethylstilbestrol, the diethylstilbestrol and the hexestrol. By the method, the problems that a target object and matrix combination situation is not completely consistent to a real detecting sample, so that treatment results of extracting, purifying and the like are quite different from daily analysis samples are solved. The standard sample is a material standard sample, is uniform and stable, does not contain a preservative or an antioxidant, is easy to store, and is used for proficiency testing of a non-steroidal anabolic hormones drug residue testing item, verification of a detecting method, calibration of a testing instrument, and quality control and assessment of a testing result.

The invention provides a diethylstilbestrol (DES) mimic antigenic epitope peptide, and a preparation method and an application thereof. Firstly, based on a phage display peptide library technology, ananti-DES monoclonal antibody is used as a target, and a DES antigen mimic epitope having the sequence of YVYPQQK, YVYPPGP or YVYPQSR is selected from a phage random display heptapeptide library. Centering on immune binding characteristics of the antigen mimic epitope, a specific detection method is designed based on enzyme-linked immunosorbent assay, colloidal gold immunochromatography and fluorescent microsphere immunochromatography. The antigenic mimic epitope has similar immunoreactivity characteristics to those of natural DES molecules, can replace an expensive DES standard substance having teratogenic and carcinogenic effects to be used in immunological detection of DES; the detection results are accurate and the sensitivity is high. The harm to human body health in the DES detectionprocess is effectively reduced, and meanwhile, the cost is saved.

The invention discloses a photo-electrochemical diethylstilbestrol biosensor constructed based on a boron-doped poly-metallic oxide as well as preparation and application thereof. In a preparation method, firstly, a two-dimension nano material g-C3N4 is modified on ITO conductive glass by adopting an electro-deposition method; then, an iron cobalt hydroxide is subjected to in-situ growth by adopting a hydrothermal method, and further, a working electrode modified by a g-C3N4-loaded boron-doped iron cobalt oxide Bi@NixFe1-xO3 / g-C3N4 is obtained; finally, a diethylstilbestrol antibody is loaded by utilizing good biocompatibility and large specific surface area of the material. During detection, as the boron-doped iron cobalt oxide Bi@NixFe1-xO3 / g-C3N4 can catalyze L-ascorbic acid-2-trisodium phosphate AAP, L-ascorbic acid AA is generated in situ, and further, an electron donor is provided for photoelectric detection; by utilizing the effect of specific quantitative binding of the antibody and an antigen on electronic transmission capacity, the photocurrent strength is correspondingly reduced, and the construction of a photoelectric sensor for detecting diethylstilbestrol by adopting an unmarked photo-electrochemical method is finally realized.

The invention discloses a method for measuring content of 11 illegally added medicaments in veterinary drug powder. The method comprises the following steps of (1) pre-treating veterinary drug powder to be measured by adopting an organic solvent ultrasonic extraction method, wherein an organic solvent is methanol-water, and a volume ratio of methanol to water is (0.9-1.1):(0.9-1.1); (2) detecting olaquindox, ribavirin, amantadine, rimantadine, dexamethasone, chloramphenicol, diethylstilbestrol, furaltodone, nitrofurantoin, nitrofural and furazolidone in extract by adopting liquid chromatography-tandem mass spectrometry. According to the method, a sample can be simply pre-treated by adopting methanol / water (50+50) as an extraction reagent for ultrasonic extraction, and can be loaded on a machine for detection after passing through a filter membrane, and a recovery rate is high; the method is lower in detection limit, a detection limit of the olaquindox, ribavirin, amantadine, rimantadine, dexamethasone, chloramphenicol, furaltodone and furazolidone is 50mu g / kg, and a detection limit of the nitrofurantoin, nitrofural and diethylstilbestrol is 100mu g / kg.

The invention discloses a method for determining diethylstilbestrol by adopting up-conversion luminescence immunochromatography. The method is characterized in that according to the research, a solvothermal method is adopted to synthesize up-conversion nanoparticles (UCNPs)-NaYF4: Yb, Er, surface functionalization modification is carried out, the nanoparticles are coupled with a diethylstilbestrol(DES) monoclonal antibody to prepare a fluorescent probe, a nitrocellulose film is respectively sprayed on a bovine serum albumin-diethylstilbestrol (BSA-DES) conjugate and a goat anti-mouse secondary antibody to form a test strip detection line (T line) and a quality control line (C line), and an up-conversion luminescence immunochromatographic test strip is established for rapid quantitative detection; a method of DES is adopted; the experimental result shows that the DES linear quantitative detection range of the test strip is 25 to 10000 ng / mL (Y is equal to 0.43927 x-0.57647, and R2 is equal to 0.996), the detection limit is 20.84 ng / mL, the detection time of a single sample is 15 min, intra-batch and inter-batch variable coefficients are both smaller than 10%, the specificity recognition capacity is high, an average value of RSD detection values is about 15% after the kit is stored at 37 DEG C for 7 days; standard recovery experiments show that the average recovery rate is 90.1%-115.2%, relative standard deviation is smaller than 5%, and the method has good consistency with high performance liquid chromatography.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium dioxide-based two-dimensional nanocomposite material. It belongs to the technical field of new nanometer functional materials and biosensors. The present invention firstly prepares a novel two-dimensional sheet-like magnetic nano-photosensitive material FeCo-N@TiO2, utilizes the good biocompatibility, large specific surface area and ferromagnetism of the material, loads the diethylstilbestrol antibody, and then passes the pentylstilbestrol antibody The cross-linking effect of dialdehyde immobilizes horseradish peroxidase. During detection, since horseradish peroxidase can catalyze hydrogen peroxide, an electrochemical signal is generated, and then the specific quantitative combination of antibody and antigen is used to transfer electrons. Influenced by the ability, the current intensity is correspondingly reduced, and finally an electrochemical biosensor for detecting diethylstilbestrol with low cost, high sensitivity, good specificity, rapid detection and simple preparation is obtained.

The invention provides a method for detecting diethylstilbestrol hormones in liquid milk according to a liquid chromatograph-mass spectrometer method. A sample pretreatment method comprises the following steps: weighing 2 g of to-be-detected liquid milk, adding 3mL of acetonitrile, carrying out vortex mixing for 20-40 seconds, carrying out centrifugation at 4000-5000 r / min for 3-8 min, and transferring the supernate A, so as to obtain the supernate A; adding 2 mL of acetonitrile into the remaining precipitate, carrying out vortex mixing for 20-40 seconds, carrying out centrifugation at 4000-5000 r / min for 3-8 min, and transferring the supernate B, so as to obtain the supernate B; combining the supernate A and the supernate B, and then adding 5 mL of water for uniform mixing; finally, adjusting the pH value of the obtained mixture to 10.9-11.1 by utilizing 0.1-0.2 mol / L of NaOH, so as to obtain an extraction solution; conducting solid-phase extraction column purification on the extraction solution, so as to obtain the pretreated to-be-detected liquid milk. The method is simple and quick to operate, and both the detection and the recovery rate can meet the detection requirements.

The invention provides a method for detecting diethylstilbestrol residues in milk and milk powder. The method comprises the following steps: pretreating a to-be-detected sample, and then carrying out HPLC-MS / MS detection; and carrying out acidification acetonitrile extraction, QuEChERS technology purification and redissolution on a target compound, and directly measuring on a machine, wherein the pretreatment operation is simple. According to the invention, the optimized method is low in matrix effect, and experimental steps are simplified; and the method is simple, convenient and sensitive, and is suitable for measuring the diethylstilbestrol residual quantity in milk and milk powder, so that the method has a good practical application value.

The invention relates to a preparing method of a qi-stagnancy and blood stasis uterus myoma model. Unpredictable stimulation is given while diethylstilbestrol liquid medicine and progesterone injection are given. The uterus myoma model can be established effectively, and the preparing method is safe, capable of being used for screening related drugs and good in application prospects.

The invention discloses a photo-electrochemical diethylstilbestrol biosensor constructed based on a boron-doped poly-metallic oxide as well as preparation and application thereof. In a preparation method, firstly, a two-dimension nano material g-C3N4 is modified on ITO conductive glass by adopting an electro-deposition method; then, an iron cobalt hydroxide is subjected to in-situ growth by adopting a hydrothermal method, and further, a working electrode modified by a g-C3N4-loaded boron-doped iron cobalt oxide Bi@NixFe1-xO3 / g-C3N4 is obtained; finally, a diethylstilbestrol antibody is loaded by utilizing good biocompatibility and large specific surface area of the material. During detection, as the boron-doped iron cobalt oxide Bi@NixFe1-xO3 / g-C3N4 can catalyze L-ascorbic acid-2-trisodium phosphate AAP, L-ascorbic acid AA is generated in situ, and further, an electron donor is provided for photoelectric detection; by utilizing the effect of specific quantitative binding of the antibody and an antigen on electronic transmission capacity, the photocurrent strength is correspondingly reduced, and the construction of a photoelectric sensor for detecting diethylstilbestrol by adopting an unmarked photo-electrochemical method is finally realized.

The invention discloses the application of vanillin as a redox medium in improving the degradation rate of diethylstilbestrol by laccase, and the vanillin concentration is 0.01-1mmol / L. Also disclosed is a method for improving the degradation rate of diethylstilbestrol by laccase with vanillin as a redox medium, comprising the following steps: Step 1: dissolving laccase in acetic acid-sodium acetate buffer to prepare laccase mother liquor; dissolving diethylstilbestrol in methanol to prepare into a diethylstilbestrol solution; dissolving vanillin in acetic acid-sodium acetate buffer to prepare a vanillin solution; step 2: after mixing the prepared laccase mother liquor and vanillin solution into a laccase-vanillin solution, mix it with the prepared diethylstilbestrol solution Mixed in the reaction system, placed in a shaking incubator for reaction; Step 3: After half an hour of reaction, use ethyl acetate to extract to terminate the diethylstilbestrol reaction and complete the degradation. The invention uses vanillin as a reducing medium, which can greatly improve the degradation rate of DES degraded by laccase and reduce the degradation cost.

The invention relates to the technical field of separation and analysis, and particularly discloses a preparation method and application of a diethylstilbestrol magnetic molecularly imprinted polymer based on an organic-inorganic hybrid technology. The present invention adopts co-precipitation method to prepare ferric oxide particles, adopts stober method to prepare silanized ferric oxide, adopts bulk polymerization method to prepare organic-inorganic hybrid diethylstilbestrol magnetic molecularly imprinted polymer, grinds and sieves the polymer, and adds The eluent is extracted and eluted and then dried. The diethylstilbestrol magnetic molecularly imprinted polymer synthesized by the present invention can quickly bind the target diethylstilbestrol on its surface, and rapidly separate it from the reaction system under the action of an external magnetic field, so as to efficiently and highly specifically remove trace amounts of diethylstilbestrol from the reaction system. For the purpose of separation in complex matrices, the efficiency of sample pretreatment is greatly improved.