Method for improving degradation rate of laccase for degrading diethylstilbestrol by using acetosyringone, and application thereof

A technology of acetosyringone and diethylstilbestrol, which is applied in the field of environmental pollution technology treatment, can solve the problems of low degradation efficiency, achieve the effect of simple operation method, improve degradation efficiency, and overcome steric hindrance and kinetic limitations

Active Publication Date: 2020-03-17
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the process of catalytic degradation of laccase, there is still the problem of low degradation efficiency. Therefore

Method used

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  • Method for improving degradation rate of laccase for degrading diethylstilbestrol by using acetosyringone, and application thereof
  • Method for improving degradation rate of laccase for degrading diethylstilbestrol by using acetosyringone, and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 draws diethylstilbestrol standard curve

[0033] Diethylstilbestrol mother liquor with a concentration of 2.5g / L was diluted to obtain standard solutions of diethylstilbestrol with a concentration of 1mg / L, 5mg / L, 10mg / L, 20mg / L, 30mg / L, and 50mg / L respectively, and the HPLC detection method was used to draw Diethylstilbestrol standard curve, such as figure 1 shown.

Embodiment 2

[0035] (1) Mix the 1mg / mL laccase solution of 0.5ml, the 0.01mol / L acetosyringone solution of 0ml and the 2.5g / L diethylstilbestrol standard solution of 0.4ml, and dilute to the volume with acetic acid-sodium acetate buffer solution 10ml, placed in a 55°C shaking incubator for reaction;

[0036] (2) After reacting for 0.5 h, extract the reaction solution twice with ethyl acetate, extract the organic phase and then rotary evaporate to dryness, add methanol to constant volume, and use vacuum filter membrane to sample;

[0037] (3) carry out concentration measurement to the sample after reaction by HPLC detection method, according to formula, degradation rate=(C 0 -C) / C 0 ×100%, the calculated degradation rate of diethylstilbestrol is 44.56%.

Embodiment 3

[0039](1) Mix 0.5ml of 1mg / mL laccase solution, 0.1ml of 0.01mol / L acetosyringone solution and 0.4ml of 2.5g / L diethylstilbestrol standard solution, and dilute to volume with acetic acid-sodium acetate buffer solution to 10ml, put it in a shaking incubator at 55°C for reaction;

[0040] (2) After reacting for 0.5 h, extract the reaction solution twice with ethyl acetate, extract the organic phase and then rotary evaporate to dryness, add methanol to constant volume, and use vacuum filter membrane to sample;

[0041] (3) carry out concentration measurement to the sample after reaction by HPLC detection method, according to formula, degradation rate=(C 0 -C) / C 0 ×100%, the calculated degradation rate of diethylstilbestrol is 83.62%.

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Abstract

The invention discloses a method for improving the degradation rate of laccase for degrading diethylstilbestrol by using acetosyringone, and an application thereof, and belongs to the technical fieldof environmental pollution treatment. The method comprises the following steps: forming an acetosyringone-laccase medium buffer solution from acetosyringone and laccase, mixing the acetosyringone-laccase medium buffer solution with a diethylstilbestrol solution, performing a reaction in a shaking incubator, and quenching the reaction by using ethyl acetate to realize the degradation of the diethylstilbestrol. The acetosyringone is used as a redox medium for electron shuttle between laccase and a substrate, so that the steric hindrance of laccase is overcome, thereby the laccase can be well combined with the diethylstilbestrol, and the degradation efficiency of the laccase is remarkably improved. The method is simple and efficient to operate, and has extremely high application values in environmental pollution treatment.

Description

technical field [0001] The invention relates to the technical field of environmental pollution treatment, in particular to a method and application for improving the degradation rate of diethylstilbestrol by laccase by using acetosyringone. Background technique [0002] Diethylstilbestrol (DES) is a synthetic non-steroidal estrogen or xenoestrogen, which has stronger estrogenic activity than estradiol (Korach et al., 1978; Shang et al., 2014). After being synthesized by a British chemist in 1938, it was initially used to treat gastrointestinal discomfort, dizziness and skin redness. Later, it was widely used in the medical field as a prescription drug to prevent miscarriage, and was also used to treat advanced prostate cancer, breast cancer, ovarian insufficiency, amenorrhea, uterine hypoplasia, dysfunctional uterine bleeding and other diseases (Goyal et al., 2001; Grenader et al., 2014; Koong et al., 2014). Diethylstilbestrol was banned in 1971 because it was found to ind...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/28
CPCA62D3/02A62D2101/28
Inventor 刘红艳张文强谢世伟李霞海洪单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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