Method for preparing diethylstilbestrol molecularly imprinted electrochemical sensor based on electropolymerization of p-aminothiophenol film and application thereof

A technology of mercaptoaniline membrane and diethylstilbestrol, which is applied in scientific instruments, material analysis through electromagnetic means, instruments, etc., to achieve the effects of short time consumption, good stability and fast response

Active Publication Date: 2018-12-07
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF4 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of diethylstilbestrol molecularly imprinted electrochemical sensors based on carbon nanotubes and go

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing diethylstilbestrol molecularly imprinted electrochemical sensor based on electropolymerization of p-aminothiophenol film and application thereof
  • Method for preparing diethylstilbestrol molecularly imprinted electrochemical sensor based on electropolymerization of p-aminothiophenol film and application thereof
  • Method for preparing diethylstilbestrol molecularly imprinted electrochemical sensor based on electropolymerization of p-aminothiophenol film and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0055] Example 1 A preparation method of diethylstilbestrol molecularly imprinted electrochemical sensor

[0056] A preparation method of the diethylstilbestrol molecularly imprinted electrochemical sensor in this embodiment, such as figure 1 As shown, proceed in sequence according to the following steps:

[0057] (11) Pretreatment of glassy carbon electrode

[0058] Use 0.5μm, 0.3μm, 0.05μm Al 2 O 3 The powder is polished on the chamois leather, rinsed with ultrapure water and then ultrasonically washed in absolute ethanol and ultrapure water, and then the electrode is placed in 0.5mol / L H 2 SO 4 In solution, cyclic scanning;

[0059] After taking out the electrode for washing, place it in the electroactive probe solution, where the electroactive probe is potassium ferricyanide / potassium ferrocyanide, and the electroactive probe solution is 5mmol / L potassium ferricyanide / ferrocyanide Potassium and 1mol / L potassium chloride solution are prepared according to the volume ratio of 1:1;

...

Example Embodiment

[0074] Example 2-6 Preparation method of diethylstilbestrol molecularly imprinted electrochemical sensor

[0075] This example is a preparation method of a diethylstilbestrol molecularly imprinted electrochemical sensor. The preparation steps and technical parameters are the same as those in Example 1, except that the corresponding technical parameters are different during the preparation process. The specific results are shown in Table 1.

[0076] Table 1 Technical parameters of the preparation process

[0077]

[0078] The potential range of the cycle scanning in steps (x1) (x=2-6) and steps (x2) (x=2-6) in the above embodiment 2-6 are both -0.3~1.2V; where multi-walled carbon nanotubes The method and amount of modified electrode with nano gold are the same as in Example 1.

[0079] The sensor prepared in Examples 2-6 has strong selectivity, good stability, high sensitivity, low cost, fast response, simple preparation steps, and easy control. Compared with other diethylstilbestrol d...

Example Embodiment

[0080] Example 7 Modification condition selection experiment of carboxylated multi-walled carbon nanotubes

[0081] In order to achieve the best modification effect of multi-walled carbon nanotubes, the dosage of the multi-walled carbon nanotube dispersion reagent and modifier was studied in this example. Among them, DMF, a reagent with good dispersion effect on MWCNTs, is combined with chitosan with good crosslinking effect, and the MWCNTs-DMF dispersion is combined with CS-1%CH 3 The COOH solution is mixed in equal volume to obtain CMWCNTs modifier. A proper amount of CMWCNTs modifier is dripped onto the electrode surface, and CMWCNTs can be evenly distributed.

[0082] In order to make the CMWCNTs modifier stick to the surface of glassy carbon stably without affecting the catalysis of CMWCNTs, in this example, CS-1%CH 3 The concentration of CS in COOH solution was studied. Such as figure 2 As shown, we compare 200mg, 300mg, 400mg, 500mg, 600mg CS in 20mL CS-1%CH respectively. 3...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing a diethylstilbestrol molecularly imprinted electrochemical sensor based on electropolymerization of a p-aminothiophenol film. The method comprises sequential steps such as glassy carbon electrode pretreatment, electrode modification by multi-wall carbon nanotubes, electrode modification by nano gold, functional monomer self-assembly, template molecular pre-assembly, electropolymerization of the p-aminothiophenol film, and template molecule elution, and an organic phase as a polyelectrolyte solvent improves the number and selectivity of binding sites in a polymer film, and is beneficial to formation of a stable host-guest compound from a template molecule and a function monomer, so that the template molecule can maintain a good rigid structurein a polymer network structure and form a cavity with a memory effect. The preparation method is simple and easy to control, and the sensor has strong selectivity and stability, high sensitivity and low cost. The sensor is suitable for analysis and detection of diethylstilbestrol residues in animal-derived foods, feeds and other samples.

Description

technical field [0001] The invention belongs to the fields of molecular imprinting technology, supramolecular chemistry and electrochemical analysis, and relates to a preparation method and application of a molecular imprinted electrochemical sensor, in particular to preparation of diethylstilbestrol molecular imprinted electrochemical sensor based on electropolymerized p-mercaptoaniline membrane method and its application. Background technique [0002] Diethylstilbesrtol (Diethylstilbesrtol) is a synthetic non-steroidal estrogen, which was born in the 1940s. Its pharmacology and efficacy are the same as the natural estrogen estradiol, so it is favored in the field of medicine, mainly as an oral hormone It is used to treat low estrogen and female physiological diseases caused by estrogen imbalance in the body. It can also treat prostate cancer and promote the metabolism of five major nutrients. In addition, diethylstilbestrol is used as an animal feed additive to promote ani...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 高文惠赵玲钰高林
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap