Intracranial aneurysm marker ODAM and application thereof

A technique for intracranial aneurysm and product expression, applied in the field of tumor markers, to achieve the effect of strong selectivity, strong specificity and clear results

Inactive Publication Date: 2016-03-02
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenesis of intracranial aneurysms has been debated for many

Method used

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  • Intracranial aneurysm marker ODAM and application thereof
  • Intracranial aneurysm marker ODAM and application thereof
  • Intracranial aneurysm marker ODAM and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The collection of embodiment 1 case

[0036] All tissue samples came to the neurosurgery department of the hospital from October 2012 to May 2014 in patients with intracranial aneurysms diagnosed by DSA, and underwent microsurgical craniotomy. The aneurysm tissue samples were the aneurysm wall tissue removed during microsurgery, and the control normal cerebrovascular tissue samples were the superficial temporal artery (STA) and / or middle meningeal artery (MMA) of brain tumor craniotomy patients at the same period. In order to minimize the differences between samples, all aneurysm tissue samples were selected from saccular aneurysms. The obtained tissue samples were immediately placed in a liquid nitrogen tank.

Embodiment 2

[0037] Example 2 extracts the total RNA in the sample

[0038] 1) Put small pieces of aneurysm (normal control blood vessel) tissue in a petri dish, add 1ml Trizol, cut it into pieces with ophthalmic scissors, and transfer to a homogenizer for homogenization;

[0039] 2) After the homogenization is completed, take out the homogenate and place it in an EP tube, set the volume to 1ml, and store it at -70°C;

[0040] 3) Place at 15-30°C for 5 minutes, add 200 μl chloroform, shake vigorously for 15 sec, and place at 15-30°C for 10 minutes;

[0041] 4) 2-8°C, centrifuge at lower than 12000g / min for 10min, discard the supernatant, add 1ml of 75% ethanol, centrifuge at lower than 7500g / min for 5min, discard the supernatant, and spot off;

[0042] 5) After the precipitate is dried (it is translucent, do not completely dry, otherwise the stability will be reduced), dissolve it with RNase-freewater at 55-60°C, and freeze it at -70°C.

Embodiment 3

[0043] Example 3 High-throughput sequencing and analysis

[0044] After RNA extraction, agarose gel electrophoresis is performed. From the results of electrophoresis, it can be preliminarily judged whether the quality of the extracted RNA sample is qualified or not, and whether it can be used for further transcriptome sequencing. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2. The qualified samples were sent to the sequencing company for sequencing. The sequencing platform was the HiSeq2500 high-throughput sequencing platform of Illumina Company for high-throughput transcriptome deep sequencing. The results of data analysis provided by the sequencing company were combined with the literature to screen the differentially expressed gene ODAM.

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Abstract

The invention relates to an intracranial aneurysm marker ODAM and application thereof, in particular to a marker ODAM gene and application of expression products in preparing diagnosis and treatment preparations for intracranial aneurysm. High-throughput sequencing is carried out on intracranial aneurysm cases by the inventor, candidate genes ODAM with low expression are selected from genes with obvious difference expression, and low expression of ODAM in intracranial aneurysm tissue is verified through further molecular verification. By means of the intracranial aneurysm marker ODAM and the application thereof, a new monitoring and treating target of intracranial aneurysm is provided, and significant clinical application value is achieved.

Description

technical field [0001] The present invention relates to tumor markers, in particular to intracranial aneurysm marker ODAM and its application, and more specifically to the application of the marker ODAM gene and its expression product in the preparation of intracranial aneurysm diagnosis and treatment preparations. Background technique [0002] Intracranial aneurysm (IA) refers to the structural destruction of the intracranial artery wall caused by various factors, resulting in abnormal swelling of the artery wall. The incidence of intracranial aneurysms is relatively high. The survey results show that congenital aneurysms account for most of the population, and the most common ones are 40-66 years old. About 10% of patients die before admission, and the autopsy detection rate is about 1-5. %, the 30-day mortality rate after intracranial aneurysm rupture and bleeding is 45%, and 30% of the survivors have varying degrees of disability. In addition, there are still more than 5...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68A61K45/00A61P9/00
CPCA61K45/00C12Q1/6883C12Q2600/158G01N33/6893G01N2333/47G01N2800/329
Inventor 李志立黄光富陈隆益谭海斌王振宇石毅刘泠殷成王奇
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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