Application of vanillin as redox medium in increasing diethylstilbestrol degradation rate of laccase

A technology of diethylstilbestrol and vanillin, which is applied in the field of environmental pollution treatment, can solve problems such as insignificant effect, slow down photodegradation, and poor effect, and achieve the effects of overcoming steric hindrance and kinetic limitations, simple operation, and low cost

Active Publication Date: 2020-03-17
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Lin et al. used ozone oxidation to degrade DES, but the effect was not significant (Lin et al., 2009)
Xu et al. studied the UV conversion of diethylstilbestrol and its persistent pollution to surface water under sunlight, and found that DES can be photocatalytically degraded, but also found that DES is a photochromic compound whose UV-induced intermediate

Method used

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  • Application of vanillin as redox medium in increasing diethylstilbestrol degradation rate of laccase
  • Application of vanillin as redox medium in increasing diethylstilbestrol degradation rate of laccase

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Draw Diethylstilbestrol Standard Curve

[0032] Diethylstilbestrol mother liquor with a concentration of 2.5g / L was diluted to obtain standard solutions of diethylstilbestrol with a concentration of 1mg / L, 5mg / L, 10mg / L, 20mg / L, 30mg / L, and 50mg / L respectively, and the HPLC detection method was used to draw Diethylstilbestrol standard curve, such as figure 1 shown.

[0033] HPLC detection conditions are chromatographic column: ZORBAX SB-C18 (150mm×4.6mm×5μm); detection conditions are: injection volume 10μL, elution with equal volume of acetonitrile and water as flow equal gradient for 10min, pump flow rate is 1.0mL / min, column temperature: 30°C, the ultraviolet detector detects the peak at 240nm wavelength, and records the peak area value.

Embodiment 2

[0035] (1) Mix 0.5mL 1mg / mL laccase mother solution, 0mL 0.01mol / L vanillin solution and 0.4mL 2.5g / L DES mother solution, supplement to 10mL with acetic acid-sodium acetate buffer solution, and place at 55°C , 120rpm shaking incubator reaction.

[0036] (2) After reacting for half an hour, use ethyl acetate to extract the solution twice, extract the organic phase and then evaporate to dryness, use methanol to constant volume, use vacuum filter membrane to sample, and measure the concentration of the reacted sample by HPLC. Measure and calculate the degradation rate.

[0037] Degradation rate calculation formula: degradation rate = (C 0 -C) / C 0 ×100%, where C 0 is the initial concentration and C is the final concentration.

[0038] After calculation, the degradation rate of this embodiment is 44.56%.

Embodiment 3

[0040](1) Mix 0.5mL 1mg / mL laccase mother solution, 0.1mL 0.01mol / L vanillin solution and 0.4ml 2.5g / L DES mother solution, supplement to 10mL with acetic acid-sodium acetate buffer solution, and place at 55°C , 120rpm shaking incubator reaction.

[0041] (2) After reacting for half an hour, use ethyl acetate to extract the solution twice, extract the organic phase and then evaporate to dryness, use methanol to constant volume, use vacuum filter membrane to sample, and measure the concentration of the reacted sample by HPLC. Measure and calculate the degradation rate.

[0042] Degradation rate calculation formula: degradation rate = (C 0 -C) / C 0 ×100%, where C 0 is the initial concentration and C is the final concentration.

[0043] After calculation, the degradation rate of this embodiment is 52.25%.

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Abstract

The invention discloses an application of vanillin as a redox medium in increasing the diethylstilbestrol degradation rate of laccase. The concentration of vanillin is 0.01-1 mmol/L. The invention also discloses a method for increasing the diethylstilbestrol degradation rate of laccase by taking vanillin as the redox medium. The method comprises the following steps: 1, dissolving the laccase intoan acetic acid-sodium acetate buffer solution to prepare a laccase mother liquor, dissolving diethylstilbestrol in methanol to prepare a diethylstilbestrol solution, and dissolving vanillin in the acetic acid-sodium acetate buffer solution to prepare a vanillin solution; 2, mixing the prepared laccase mother liquor with the vanillin solution to obtain a laccase-vanillin solution, mixing the laccase-vanillin solution with the prepared diethylstilbestrol solution in a reaction system, putting the reaction system into a shaking incubator, and carrying out a reaction; and 3, extracting with ethylacetate extraction after the reaction is performed for half an hour in order to terminate the diethylstilbestrol reaction and complete degradation. The method adopting the vanillin as the reducing medium greatly increases the DES degradation rate of laccase, and reduces the degradation cost.

Description

technical field [0001] The invention relates to the technical field of environmental pollution treatment, in particular to the application of vanillin as a redox medium in improving the degradation rate of diethylstilbestrol by laccase. Background technique [0002] Diethylstilbestrol (DES) is a synthetic non-steroidal estrogen or xenoestrogen, which has stronger estrogenic activity than estradiol (Korach et al., 1978; Shang et al., 2014). After being synthesized by a British chemist in 1938, it was initially used to treat gastrointestinal discomfort, dizziness and skin redness. Later, it was widely used in the medical field as a prescription drug to prevent miscarriage, and was also used to treat advanced prostate cancer, breast cancer, ovarian insufficiency, amenorrhea, uterine hypoplasia, dysfunctional uterine bleeding and other diseases (Goyal et al., 2001; Grenader et al., 2014; Koong et al., 2014). Diethylstilbestrol was banned in 1971 because it was found to induce ...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/28
CPCA62D3/02A62D2101/28
Inventor 刘红艳张文强唐琳谢世伟李培骏单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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