Application of vanillin as redox medium in increasing diethylstilbestrol degradation rate of laccase

Abstract

The invention discloses an application of vanillin as a redox medium in increasing the diethylstilbestrol degradation rate of laccase. The concentration of vanillin is 0.01-1 mmol / L. The invention also discloses a method for increasing the diethylstilbestrol degradation rate of laccase by taking vanillin as the redox medium. The method comprises the following steps: 1, dissolving the laccase intoan acetic acid-sodium acetate buffer solution to prepare a laccase mother liquor, dissolving diethylstilbestrol in methanol to prepare a diethylstilbestrol solution, and dissolving vanillin in the acetic acid-sodium acetate buffer solution to prepare a vanillin solution; 2, mixing the prepared laccase mother liquor with the vanillin solution to obtain a laccase-vanillin solution, mixing the laccase-vanillin solution with the prepared diethylstilbestrol solution in a reaction system, putting the reaction system into a shaking incubator, and carrying out a reaction; and 3, extracting with ethylacetate extraction after the reaction is performed for half an hour in order to terminate the diethylstilbestrol reaction and complete degradation. The method adopting the vanillin as the reducing medium greatly increases the DES degradation rate of laccase, and reduces the degradation cost.

Description

technical field [0001] The invention relates to the technical field of environmental pollution treatment, in particular to the application of vanillin as a redox medium in improving the degradation rate of diethylstilbestrol by laccase. Background technique [0002] Diethylstilbestrol (DES) is a synthetic non-steroidal estrogen or xenoestrogen, which has stronger estrogenic activity than estradiol (Korach et al., 1978; Shang et al., 2014). After being synthesized by a British chemist in 1938, it was initially used to treat gastrointestinal discomfort, dizziness and skin redness. Later, it was widely used in the medical field as a prescription drug to prevent miscarriage, and was also used to treat advanced prostate cancer, breast cancer, ovarian insufficiency, amenorrhea, uterine hypoplasia, dysfunctional uterine bleeding and other diseases (Goyal et al., 2001; Grenader et al., 2014; Koong et al., 2014). Diethylstilbestrol was banned in 1971 because it was found to induce ...

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Problems solved by technology

[0003] Lin et al. used ozone oxidation to degrade DES, but the effect was not significant (Lin et al., 2009)

Xu et al. studied the UV conversion of diethylstilbestrol and its persistent pollution to surface water under sunlight, and found that DES can be photocatalytically degraded, but also found that DES is a photochromic compound whose UV-induced intermediate can Conversion back to DES in sunlight, which apparently slows down the photodegradation of DES (Xu et al., 2017)

As a kind of fat-soluble compound DES is not easily degraded in the environment, the traditional treatment methods to degrade this kind of phenolic organic pollutants are not only expensive but also ineffective (Snyder et al., 2003; Tay et al., 2009)

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