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A preparation method and application of an electrochemical diethylstilbestrol sensor based on a titanium dioxide-based two-dimensional nanocomposite material

A technology of diethylstilbestrol and titanium dioxide, which is applied in the direction of scientific instruments, analytical materials, and material analysis by electromagnetic means, can solve the problems of unfavorable practical application, poor conductivity, and low sensitivity of electrochemical sensors, so as to increase electron transfer capacity and improve Effects of strength and stability, enhancement of resonance energy transfer capability

Inactive Publication Date: 2018-02-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemical sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 FeCo-N@TiO 2 preparation of

[0035]First, add 0.8 mmol of iron salt, 0.8 mmol of cobalt salt and 1 mmol of ammonium salt into 5 mL of tetrabutyl titanate, slowly add 0.5 mL of hydrofluoric acid during stirring, and react in the reactor at 160 °C for 24 hours , cooled to room temperature, centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50 °C; then, the ground powder was put into a muffle furnace with a heating rate of 1 °C / min at 480 °C Calcined for 60 min; finally, the calcined powder was cooled to room temperature to obtain FeCo-N@TiO 2 ;

[0036] Described iron salt is ferric sulfate;

[0037] Described cobalt salt is cobalt sulfate;

[0038] Described ammonium salt is ammonium sulfate.

Embodiment 2

[0039] Example 2 FeCo-N@TiO 2 preparation of

[0040] First, add 0.8 mmol of iron salt, 1.0 mmol of cobalt salt and 1 mmol of ammonium salt into 5 mL of tetrabutyl titanate, slowly add 0.65 mL of hydrofluoric acid during stirring, and react in the reactor at 180 °C for 21 hours , cooled to room temperature, centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50 °C; then, the ground powder was put into a muffle furnace with a heating rate of 2 °C / min, at 520 °C Calcined for 30 min; finally, the calcined powder was cooled to room temperature to obtain FeCo-N@TiO 2 ;

[0041] Described iron salt is ferric chloride;

[0042] Described cobalt salt is cobalt chloride;

[0043] Described ammonium salt is ammonium chloride.

Embodiment 3

[0044] Example 3 FeCo-N@TiO 2 preparation of

[0045] First, add 0.8 mmol of iron salt, 1.2 mmol of cobalt salt and 1 mmol of ammonium salt into 5 mL of tetrabutyl titanate, slowly add 0.8 mL of hydrofluoric acid during stirring, and react in the reactor at 200 °C for 18 hours , after cooling to room temperature, centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50°C; then, the ground powder was put into a muffle furnace with a heating rate of 3°C / min, at 560°C Calcined for 10 min; finally, the calcined powder was cooled to room temperature to obtain FeCo-N@TiO 2 ;

[0046] Described iron salt is ferric nitrate;

[0047] Described cobalt salt is cobalt nitrate;

[0048] Described ammonium salt is ammonium nitrate.

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Abstract

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium dioxide-based two-dimensional nanocomposite material. It belongs to the technical field of new nanometer functional materials and biosensors. The present invention firstly prepares a novel two-dimensional sheet-like magnetic nano-photosensitive material FeCo-N@TiO2, utilizes the good biocompatibility, large specific surface area and ferromagnetism of the material, loads the diethylstilbestrol antibody, and then passes the pentylstilbestrol antibody The cross-linking effect of dialdehyde immobilizes horseradish peroxidase. During detection, since horseradish peroxidase can catalyze hydrogen peroxide, an electrochemical signal is generated, and then the specific quantitative combination of antibody and antigen is used to transfer electrons. Influenced by the ability, the current intensity is correspondingly reduced, and finally an electrochemical biosensor for detecting diethylstilbestrol with low cost, high sensitivity, good specificity, rapid detection and simple preparation is obtained.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical diethylstilbestrol sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Diethylstilbestrol is a synthetic non-steroidal estrogen substance. At present, the methods for detecting diethylstilbestrol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need profes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 张勇杜斌马洪敏吴丹范大伟
Owner UNIV OF JINAN
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