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High performance liquid chromatography detection method for content of cochineal in food

A high-performance liquid chromatography and cochineal technology, which is applied in the field of high-performance liquid chromatography detection of carmine content in food, can solve the problems of undiscovered and undiscovered open patent documents of high-performance liquid chromatography detection methods, etc. Achieve the effect of saving time, rapid component detection and analysis, and short detection time

Inactive Publication Date: 2020-03-17
天津量信检验认证技术有限公司 +2
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  • Description
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  • Application Information

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Problems solved by technology

[0003] Through the search of the published patent documents, no published patent documents on the high-performance liquid chromatography detection method for the content of carmine in food have been found, and no published patent documents identical to this patent application have been found.

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  • High performance liquid chromatography detection method for content of cochineal in food

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Embodiment Construction

[0032] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0033] A high-performance liquid chromatography detection method for carmine content in food, the innovation of which is that the method includes the following steps:

[0034] 1) Preparation of standard working solution:

[0035] Weigh an appropriate amount of carminic acid standard substance, dissolve it with methanol, transfer to a volumetric flask and constant volume, and prepare carminic acid standard working solution of appropriate concentration for use;

[0036] 2) Preparation of sample working solution:

[0037] Accurately weigh 0.3g to 0.5g of sample into a 50mL centrifuge tube, add 10mL of HCl extract with a concentration of 2mol / L, vortex, ultrasonicate at 75°C for 30min, cool to room temperature, add 15mL of methanol, oscillate, ...

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Abstract

The invention relates to a high performance liquid chromatography detection method for the content of cochineal in food. The method is characterized by comprising the following steps: 1) preparing a standard working solution; 2) preparing a sample working solution; and 3) carrying out detecting and analyzing based on high performance liquid chromatography. The method is scientific and reasonable in design, can accurately analyze and detect the content of the cochineal in the food, enables the treatment process before analysis to be simpler and quicker, enables component detection and analysisto be quicker and more accurate, saves time, and is high in detection sensitivity, accurate in detection data, wide in application range, cost-saving and capable of improving the detection efficiency.

Description

technical field [0001] The invention belongs to the field of food additive detection, and relates to a high-performance liquid chromatography detection method for carmine content in food. Background technique [0002] Carmine is extracted from cochineal insects growing on cactus. It is an anthraquinone natural pigment. Unlike other natural pigments, its physical and chemical properties are very stable. It is regarded as the safest natural pigment and used in food, Production of cosmetics, pharmaceuticals and textiles. At present, my country has allowed its production as a food additive. Because cochineal is a natural pigment, and its color is naturally bright, it has great advantages over synthetic pigments. At present, some large food manufacturers have begun to use it to replace synthetic pigments. As a natural pigment additive, carmine can be used in beverages, meat products, candies, aperitifs, apple cider vinegar, yogurt, baked goods, jams, jellies, etc. It has been l...

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/062G01N2030/884
Inventor 谢亚楠李悦俞涵鲁瑶尹亮朱敬华郭兴旺
Owner 天津量信检验认证技术有限公司
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