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Grading model for detecting benign and malignant degrees of skin tumors and application thereof

A skin tumor and model technology, which is applied in the measurement/examination of microorganisms, genomics, instruments, etc., can solve problems such as increasing the complexity of treatment, difficult to distinguish in situ melanoma and mole morphology, and increasing patient suffering.

Pending Publication Date: 2020-03-17
LISEN IMPRINTING DIAGNOSTICS (WUXI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early melanoma in situ can be easily resected by surgery, but melanoma in situ is difficult to distinguish from nevus in shape, which has always been a difficult point in the pathological diagnosis of melanoma. Some melanoma patients have already metastasized when they are clearly diagnosed. Increased the complexity of the treatment, but also increased the pain of the patient

Method used

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  • Grading model for detecting benign and malignant degrees of skin tumors and application thereof
  • Grading model for detecting benign and malignant degrees of skin tumors and application thereof
  • Grading model for detecting benign and malignant degrees of skin tumors and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] Imprinted gene analysis of embodiment 1 skin cancer

[0142] The detection method of the imprinted gene comprises the following steps:

[0143] (1) Obtain the tissue cell section (10 microns) of skin cancer, put into 10% neutral formalin solution and fix, in case RNA degrades, fixation time is 24 hours, paraffin embedding (FFPE), described Slides need to be detached with positive charges, and the slices are baked in a 40°C oven for more than 3 hours;

[0144] (2) Carry out dewaxing treatment according to the sample processing method of RNASCope to block the endogenous peroxidase activity in the sample, enhance permeability and expose RNA molecules;

[0145] (3) Design probes: design specific primers according to the imprinted gene sequence;

[0146] The design probes are designed according to imprinted genes Z1 (Gnas), Z6 (Plagl1), Z8 (Dcn), Z10 (Gatm), Z11 (Grb10), Z13 (Sgce) and Z16 (Snrpn / Snurf), specifically Select a sequence in the intron of each gene as a probe...

Embodiment 2

[0156] Example 2 Imprinted Gene Analysis of Skin Puncture Biopsy Samples

[0157] The skin puncture biopsy sample is to take out suspicious lesion tissue by puncture, fix with 10% neutral formalin solution for more than 24 hours, and other detection methods are the same as in Example 1.

[0158] From Figure 3(a)-Figure 3(f) It can be seen that each gene of Z1, Z6, Z8, Z10, Z11, Z13, and Z16 has a different response sensitivity to skin cancer, or in other words, the intensity and status of imprint loss corresponding to skin cancer expression are different.

[0159] Specifically, the sensitivity of each imprinted gene to skin cancer is as follows: Figure 4(a)-Figure 4(g) ,From Figure 4(a)-Figure 4(d) It can be seen that the imprinted deletion of the imprinted gene Z1 begins to appear in the malignant potential stage, and gradually rises to a higher level with the development of skin cancer, and the copy number abnormality of the imprinted gene begins to appear in the malign...

Embodiment 347

[0161] Example 3 Imprinted gene analysis of 47 cases of skin tumor samples

[0162] The tissues obtained from 47 skin cancer patients included skin biopsy samples (10 microns), and the detection method was the same as in Example 1.

[0163] From Figure 5(a)-Figure 5(g) It can be seen that the proportions of imprint deletion, copy number abnormality and total expression of the seven probes Z1, Z6, Z8, Z10, Z11, Z13 and Z16 in 47 cases of skin tumor tissue samples showed a distribution from low to high, according to For the distribution trend of different probes, we calculated the grading standard shown by the dotted line in the figure, and the imprint loss, copy number abnormality and total expression of each probe can be divided into 5 grades from low to high.

[0164] The specific classification is as follows:

[0165] It can be seen from Figure 5(a) that, for the imprinted gene Z1, the imprinted gene deletion expression is less than 15%, the abnormal expression of the cop...

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Abstract

The invention relates to a grading model for detecting benign and malignant degrees of skin tumors and application thereof. According to the model, changes of imprinting genes in tumors are graded bycalculating imprinting gene deletion expression quantity, imprinting gene copy number abnormal expression quantity and imprinting gene total expression quantity. According to the detection model and the device provided by the invention, the expression of the imprint deletion on the tissue and the cell sample of a skin tumor patient is represented by using the intuitive method, the imprint (trace)gene change is objectively, intuitively, early and accurately detected through adoption of an imprint gene in-situ labeling method, the quantitative model can be provided, and the great contribution is provided for the diagnosis of the skin tumor.

Description

technical field [0001] The present invention relates to the field of biotechnology, to the field of gene diagnosis, to a grading model for detecting the degree of benign and malignant skin tumors and its application, in particular to a grading model and its composition for a group of imprinted genes in detecting the degree of benign and malignant skin tumors installation. Background technique [0002] Skin cancer includes malignant melanoma, basal cell carcinoma, squamous cell carcinoma, eczema-like carcinoma, fibrosarcoma, etc. Skin cancers other than malignant melanoma are more common, but the degree of malignancy is relatively low, and they are not included in many countries. Cancer statistics, according to World Health Organization estimates, there are more than 3 million new cases every year. Malignant melanoma has a high degree of malignancy and is prone to systemic metastasis. According to the statistics of the World Health Organization, in 2012, there were 232,000 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20G16B5/00G16B25/10
CPCG16B25/10C12Q1/6886C12Q2600/112C12Q2600/154C12Q2600/158G16B20/10C12Q1/6888G16B5/00G16B20/20
Inventor 成彤周宁
Owner LISEN IMPRINTING DIAGNOSTICS (WUXI) CO LTD
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