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A chip device and method for simulating tumor penetration in vitro

An in vitro simulation, chip technology, applied in the methods of supporting/immobilizing microorganisms, tissue cell/virus culture devices, measuring devices, etc., can solve the problems of research, difficult drug penetration behavior, long cell spheroid cycle, etc. Efficiency, the effect of reducing the cost of animal experiments

Active Publication Date: 2021-08-17
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, there are problems such as a long period of constructing cell spheres, and the properties of cell spheres are not completely uniform.
[0006] The penetration of drugs in tumor tissue, especially the penetration of nanoparticles and macromolecular drugs in tumor tissue is an important factor affecting the effect of anti-tumor drugs. However, the penetration behavior of drugs in tumors is difficult to study in vivo

Method used

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  • A chip device and method for simulating tumor penetration in vitro
  • A chip device and method for simulating tumor penetration in vitro
  • A chip device and method for simulating tumor penetration in vitro

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1: The chip device of the first structure of the present invention

[0055] Such as figure 1 As shown, the first chip device provided by the present invention includes a chip, and the chip has the following structure: the size of the topmost PDMS chip 11 is 6mm * 6mm, and the middle layer is a glass slide (10mm in diameter and 0.17mm in thickness) Cover glass) 12; the size and shape of the PVC support layer are the same as the glass slide layer, and in the central area of ​​the PVC support layer, corresponding to the area of ​​the chamber on the PDMS chip 11, an observation window 13 of 3.5mm×2mm is opened. The center of the PDMS chip 11 coincides with the center of the glass slide 12 .

[0056] Engraved in the PDMS chip 11 as figure 1 Shown microchannel 113 and chamber 114: wherein the left microchannel is the fluid inlet 111, the right microchannel is the fluid outlet 112, and the width and height of the microchannel 113 entering and leaving the chamber a...

Embodiment 2

[0057] Embodiment 2: the chip device of the second structure of the present invention

[0058] Such as figure 2 As shown, the second chip device provided by the present invention includes a chip, and the chip has the following structure: the size of the topmost PDMS chip 21 is 12mm * 9mm, and the middle layer is a glass slide (16mm in diameter and 0.17mm in thickness) cover glass) 22; the size and shape of the PVC support layer are the same as the glass slide layer, and in the central area of ​​the PVC support layer, corresponding to the area of ​​the chamber on the PDMS chip 21, an observation window 23 of 10mm * 4mm is opened (such as attached figure 2 shown). The center of the PDMS chip 21 coincides with the center of the glass slide 22 .

[0059] Engraved in the PDMS chip 22 as figure 2 The microfluidic channels and chambers are shown. Two symmetrical chambers 214 are set on the chip; the size of the chambers is 3200 μm×2200 μm, and the height of the chambers is 20...

Embodiment 3

[0060] Embodiment 3: the chip device of the third structure of the present invention

[0061] Such as image 3 As shown, the third kind of chip device provided by the present invention includes a chip, and the chip has the following structure: the size of the topmost PDMS chip 31 is 12mm * 9mm, and the middle layer is a glass slide with a diameter of 16mm and a thickness of 0.17mm ( cover glass) 32; the size and shape of the PVC support layer are the same as the glass slide layer, on both sides of the PVC support layer, corresponding to the area of ​​the chamber on the PDMS chip 31, two observation windows 33 of 5mm×2mm are opened (Such as image 3 shown). The center of the PDMS chip 31 coincides with the center of the glass slide 32 .

[0062] engraved in the PDMS chip as image 3 The microfluidic channels and chambers are shown. Two symmetrical chambers 314 are provided on the chip; the size of the chambers is 4900 μm×1200 μm, and the height of the chambers 314 is 150 μ...

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Abstract

The invention discloses a chip device and method for simulating tumor penetration in vitro. The chip device includes a chip; the chip is provided with at least one chamber; the chamber is provided with at least one structure for fixing cell spheres and at least one baffle structure, after the fluid flows into the chamber from the fluid inlet of the chamber, the baffle structure Under the barrier of the fixed cell sphere, the fluid flows out of the chamber through the fluid outlet of the chamber; the space size of the chamber is smaller than the space size of the three-dimensional tumor cell sphere at least in one dimension, and can accommodate at least one cell sphere. The chip device of the present invention can simulate the high density and high osmotic pressure characteristics of tumor tissue to a certain extent; can reflect the characteristics of nanoparticles and macromolecules penetrating into the tumor through convective mass transfer on the tumor surface; Predicting the penetration behavior of granular drugs in tumors is of great significance for the development, evaluation and quality monitoring of macromolecular and nanoparticle anti-tumor drugs.

Description

technical field [0001] The invention relates to a chip device and method for simulating tumor infiltration in vitro, belonging to the field of medical research and development and detection equipment. Background technique [0002] The development of drugs needs to go through two stages: preclinical research and clinical research. Although clinical research is the most effective and final method for drug screening, due to factors such as safety risks, ethics, and trial time costs, it can only be carried out under the premise of ensuring the basic effectiveness and safety of drugs. Therefore, in preclinical research, more accurate prediction of drug efficacy and safety through in vitro and animal models is the basis and guarantee for clinical research. Especially in the stage of in vitro research, developing a powerful drug evaluation model to simulate the action process of drugs in vivo is an important way to speed up the speed of drug development and screening and improve t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34C12Q1/02B01L3/00
CPCB01L3/5027C12M23/16C12M25/06C12M27/20C12M41/46G01N33/5005
Inventor 蒋国强景楠
Owner TSINGHUA UNIV
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