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Chip device and method for simulating tumor permeation in vitro

An in vitro simulation and chip technology, which is applied in the direction of supporting/immobilizing microorganisms, tissue cell/virus culture devices, measuring devices, etc., can solve the problems of difficult drug penetration behavior, research, and incompletely uniform properties of cell spheroids. Achieve the effect of reducing the cost of animal experiments and improving efficiency

Active Publication Date: 2020-03-31
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, there are problems such as a long period of constructing cell spheres, and the properties of cell spheres are not completely uniform.
[0006] The penetration of drugs in tumor tissue, especially the penetration of nanoparticles and macromolecular drugs in tumor tissue is an important factor affecting the effect of anti-tumor drugs. However, the penetration behavior of drugs in tumors is difficult to study in vivo

Method used

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  • Chip device and method for simulating tumor permeation in vitro
  • Chip device and method for simulating tumor permeation in vitro
  • Chip device and method for simulating tumor permeation in vitro

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1: The chip device of the first structure of the present invention

[0055] like figure 1 As shown, the first chip device provided by the present invention includes a chip, and the chip has the following structure: the size of the topmost PDMS chip 11 is 6mm * 6mm, and the middle layer is a glass slide (10mm in diameter and 0.17mm in thickness) Cover glass) 12; the size and shape of the PVC support layer are the same as the glass slide layer, and in the central area of ​​the PVC support layer, corresponding to the area of ​​the chamber on the PDMS chip 11, an observation window 13 of 3.5mm×2mm is opened. The center of the PDMS chip 11 coincides with the center of the glass slide 12 .

[0056] Engraved in the PDMS chip 11 as figure 1 Shown microchannel 113 and chamber 114: wherein the left microchannel is the fluid inlet 111, the right microchannel is the fluid outlet 112, and the width and height of the microchannel 113 entering and leaving the chamber are ...

Embodiment 2

[0057] Embodiment 2: the chip device of the second structure of the present invention

[0058] like figure 2 As shown, the second chip device provided by the present invention includes a chip, and the chip has the following structure: the size of the topmost PDMS chip 21 is 12mm * 9mm, and the middle layer is a glass slide (16mm in diameter and 0.17mm in thickness) cover glass) 22; the size and shape of the PVC support layer are the same as the glass slide layer, and in the central area of ​​the PVC support layer, corresponding to the area of ​​the chamber on the PDMS chip 21, an observation window 23 of 10mm * 4mm is opened (such as attached figure 2 shown). The center of the PDMS chip 21 coincides with the center of the glass slide 22 .

[0059] Engraved in the PDMS chip 22 as figure 2 The microfluidic channels and chambers are shown. Two symmetrical chambers 214 are set on the chip; the size of the chambers is 3200 μm×2200 μm, and the height of the chambers is 200 μ...

Embodiment 3

[0060] Embodiment 3: the chip device of the third structure of the present invention

[0061] like image 3 As shown, the third kind of chip device provided by the present invention includes a chip, and the chip has the following structure: the size of the topmost PDMS chip 31 is 12mm * 9mm, and the middle layer is a glass slide with a diameter of 16mm and a thickness of 0.17mm ( cover glass) 32; the size and shape of the PVC support layer are the same as the glass slide layer, on both sides of the PVC support layer, corresponding to the area of ​​the chamber on the PDMS chip 31, two observation windows 33 of 5mm×2mm are opened (like image 3 shown). The center of the PDMS chip 31 coincides with the center of the glass slide 32 .

[0062] Engraved in the PDMS chip as image 3 The microfluidic channels and chambers are shown. Two symmetrical chambers 314 are provided on the chip; the size of the chambers is 4900 μm×1200 μm, and the height of the chambers 314 is 150 μm; eac...

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Abstract

The invention discloses a chip device and method for simulating tumor permeation in vitro. The chip device comprises a chip. At least one chamber is formed in the chip; and at least one cell ball fixing structure and at least one baffling structure are arranged in the chamber. After flowing into the chamber from a fluid inlet of the chamber, fluid flows through the cell ball fixing structure underthe blocking of the baffling structure and then flows out of the chamber through a fluid outlet of the chamber. The space size of the chamber is at least smaller than the space size of a three-dimensional tumor cell ball in one dimension and can at least accommodate one cell ball. According to the invention, the high-density characteristic and the high osmotic pressure characteristic of tumor tissues can be simulated to a certain degree; the characteristic that nanoparticles and macromolecules permeate into the tumor through convective mass transfer on the surface of the tumor can be reflected; and the permeation behaviors of macromolecular and nano-particle drugs in tumors can be estimated. Therefore, the chip device and method have the important significance in development, evaluation and quality monitoring of macromolecular and nano-particle anti-tumor drugs.

Description

technical field [0001] The invention relates to a chip device and method for simulating tumor infiltration in vitro, belonging to the field of medical research and development and detection equipment. Background technique [0002] The development of drugs needs to go through two stages: preclinical research and clinical research. Although clinical research is the most effective and final method for drug screening, due to factors such as safety risks, ethics, and trial time costs, it can only be carried out under the premise of ensuring the basic effectiveness and safety of drugs. Therefore, in preclinical research, more accurate prediction of drug efficacy and safety through in vitro and animal models is the basis and guarantee for clinical research. Especially in the stage of in vitro research, developing a powerful drug evaluation model to simulate the action process of drugs in vivo is an important way to speed up the speed of drug development and screening and improve t...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34C12Q1/02B01L3/00
CPCB01L3/5027C12M23/16C12M25/06C12M27/20C12M41/46G01N33/5005
Inventor 蒋国强景楠
Owner TSINGHUA UNIV
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