Duck viral hepatitis and duck reovirus disease bivalent inactivated vaccine and preparation method thereof

A dual inactivated vaccine and reovirus disease technology, applied to biochemical equipment and methods, viruses, vaccines, etc., can solve problems such as duck lameness and economic losses in duck farming

Active Publication Date: 2020-04-14
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding ducks can see swelling of the hock joints, with yellow or blood-like exudates inside, and in severe cases, there are cheese-l...

Method used

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  • Duck viral hepatitis and duck reovirus disease bivalent inactivated vaccine and preparation method thereof
  • Duck viral hepatitis and duck reovirus disease bivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1—Isolation and Identification of Type 3 Duck Hepatitis Virus

[0079] Disease material treatment Collect the liver and follicle tissues of diseased ducks from the breeding duck flocks that have experienced egg production decline, cut the liver and follicle membranes, add sterilized saline at a ratio of 1:3, grind to make a tissue suspension, freeze and thaw, Centrifuge at 12000r / min for 5min. The supernatant was collected, sterilized by filtration, and set aside.

[0080] Disease material inoculation Inoculate 5 susceptible duck embryos 10-11 days old with the above-mentioned filtrate through the allantoic cavity, 0.2ml / embryo, and continue to hatch at 37°C. Duck embryos that died within 24 hours were discarded, and then the embryos were photographed twice a day, observed continuously until the fifth day, the allantoic fluid of duck embryos that died within 24 to 120 hours was aseptically harvested, and the lesions of duck embryos were observed. The collected...

Embodiment 2

[0087] Example 2—Isolation and identification of type 1 duck hepatitis virus

[0088] Disease material treatment Collect the liver and follicle tissues of diseased ducks from the breeding duck flocks that have experienced egg production decline, cut the liver and follicle membranes, add sterilized saline at a ratio of 1:3, grind to make a tissue suspension, freeze and thaw, Centrifuge at 12000r / min for 5min. The supernatant was collected, sterilized by filtration, and set aside.

[0089] Disease material inoculation Inoculate 5 9-10-day-old SPF chicken embryos with the above-mentioned filtrate through the allantoic cavity, 0.2ml / embryo, and continue to hatch at 37°C. Duck embryos that died within 24 hours were discarded, and the embryos were photographed twice a day thereafter, observed continuously until the fifth day, the allantoic fluid of dead chicken embryos was aseptically harvested within 24 to 120 hours, and the lesions of the chicken embryos were observed. The colle...

Embodiment 3

[0096] Example 3—Isolation and identification of novel duck reovirus

[0097] Disease material treatment Collect the spleen tissue of diseased ducks from the Cherry Valley duck group with spleen necrosis, cut it into pieces, add sterilized saline at a ratio of 1:3, grind to make a tissue suspension, freeze-thaw, and centrifuge at 12000r / min for 5min . The supernatant was collected, sterilized by filtration, and set aside.

[0098] Disease material inoculation Inoculate 5 6-7 day-old susceptible chicken embryos with the above-mentioned filtrate through the yolk sac, 0.2ml / embryo, and continue to hatch at 37°C. Duck embryos that died within 24 hours were discarded, and the embryos were photographed twice a day thereafter, observed continuously until the fifth day, the allantoic fluid of dead chicken embryos was aseptically harvested within 24 to 120 hours, and the lesions of the chicken embryos were observed. The collected allantoic fluid is poisonous and cryopreserved.

[00...

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Abstract

The invention relates to a duck viral hepatitis and duck reovirus disease bivalent inactivated vaccine and a preparation method thereof. According to the invention, based on the epidemic situation ofmixed infection of duck reovirus and duck hepatitis virus in domestic waterfowl epidemic diseases, an effective coping strategy is provided, wherein separated domestic epidemic strains of a duck reovirus 17117 strain, a duck hepatitis virus type 1 16115 strain and a duck hepatitis virus type 3 16023 strain are used as vaccine preparation strains, and antigen solution preparating, inactivating, concentrating and mixing according to a certain ratio is performed to prepare the duck viral hepatitis and duck reovirus disease bivalent inactivated vaccine; and in the application of the duck viral hepatitis and duck reovirus disease bivalent inactivated vaccine, currently epidemic type 1 and type 3 duck viral hepatitis and duck reovirus diseases can be simultaneously and effectively prevented after one immunization with the vaccine.

Description

technical field [0001] The invention relates to a dual inactivated vaccine for duck viral hepatitis and duck reovirus disease, which belongs to the field of veterinary biological products. Background technique [0002] Duck viral hepatitis is an acute, highly contagious and fatal infectious disease caused by duck hepatitis virus (DHV) in ducklings, characterized by hemorrhagic inflammation of the liver. The disease can occur all year round, and only occurs in ducklings during natural outbreaks, causing huge economic losses to the breeding industry. There are three traditional duck hepatitis virus serotypes that cause duck hepatitis types 1, 2 and 3. In recent years, due to factors such as immune pressure, serotype 1 duck hepatitis virus has shown a new epidemic situation. Scholars in Taiwan and South Korea have successively isolated typical small RNA viruses with similar genome structure to DHV-1, claiming that the new Taiwanese and South Korean novels do not have cross-ne...

Claims

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Application Information

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IPC IPC(8): A61K39/29A61K39/15A61K39/39A61P31/14C12N7/00C12N7/06C12R1/93
CPCA61K39/12A61K39/39A61P31/14C12N7/00A61K2039/70A61K2039/552A61K2039/5252C12N2770/32434C12N2720/12234C12N2770/32321C12N2770/32363C12N2720/12221C12N2720/12263A61K2300/00Y02A50/30
Inventor 张青婵彭建云孟照洁郭大伟李磊贾爱琴房宝英徐龙涛王蕾张连秀
Owner QILU ANIMAL HEALTH PROD
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