Double-frame four-spectral imaging method and application thereof

Abstract

The invention relates to a double-frame four-spectral imaging method and application thereof. The method specifically comprises the following steps: sequentially switching light sources to capture twoframes of images after reaching focusing motionless of a color COMS camera; to be specific, correspondingly acquiring an RGB color channel image by superposing white light and an RGB three-channel optical filter during the obtaining of a first frame of image; and acquiring a corresponding Y-channel color image by superposing whit light and a yellow light filter during obtaining of a second frameof image. According to the double-frame four-spectral imaging method, light source switching is carried out and RGB and Y color channel spectral imaging can be successively acquired by the color COMScamera during double-frame shooting; compared with a time-sharing multi-spectral imaging system, the provided method enables the dependence on light sources to be weaker, only two light sources are needed to reduce the cost; and compared with the existing area array gray CMOS camera, the RGBY four-spectral imaging method enables image capturing time consumption to be shortened and image transmission requirement to be low; the obtained four-channel spectrum is used for cell detection, so that problems that the reliability and effectiveness of the existing manual detection result are difficult to guarantee are solved.

Description

technical field [0001] The invention relates to the technical field of image processing, in particular to a dual-frame four-spectrum imaging method and application. Background technique [0002] Malignant lesions of epithelial cells are the most common lesions. In pathology, exfoliative cytology is usually used for special staining, and then from a morphological point of view, it is completed by manual inspection of microscopic images of sample smears or by computer image processing techniques. [0003] The manual detection method has simple equipment, easy operation, and low cost, and is currently the main method for the diagnosis of malignant lesions of epithelial cells. However, this manual method is time-consuming and labor-intensive for inspectors, so it is difficult to guarantee the reliability and validity of manual inspection results. [0004] Due to the low efficiency of manual morphological analysis, combined with the relevant knowledge of digital image processing...

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Problems solved by technology

[0005] In order to overcome the above-mentioned deficiencies in the prior art, the present invention proposes a dual-frame four-spectrum imaging method, which can not only solve the problem that the existing time-sharing multi-spectral imaging system has a strong dependence on the light source, and the problem that the light source is easily damaged and the cost is high, but also It can solve the technical problems of time-consuming capture of four color channels and high requirements for image transmission in area-array grayscale CMOS cameras

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