A method for constructing the ULC characteristic map of the soil fritillaria medicinal material and its component content determination method

A technology of characteristic map and construction method, which is applied in the field of construction of UPLC characteristic map of Tubeimu medicinal material, can solve the problems of difficulty in fully reflecting the internal quality of the medicinal material, limited separation ability, lack of specificity, etc., and achieve stable specificity, fast method, and good reproducibility

Active Publication Date: 2021-09-07
GUANGDONG YIFANG PHARMA
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The 2015 edition of the "Chinese Pharmacopoeia" only uses tulimoside A as the content determination index. Due to the diversity and complexity of active ingredients in traditional Chinese medicine, the content determination of a single component is difficult to fully reflect the internal quality of the medicinal material, and it also lacks specificity
At present, the research on fingerprint / characteristic spectrum and content determination of Fritillaria adopts high performance liquid chromatography (HPLC method). This method takes a long time, has low sensitivity and limited separation ability, and can no longer solve the complex analysis process of traditional Chinese medicine. practical problems in

Method used

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  • A method for constructing the ULC characteristic map of the soil fritillaria medicinal material and its component content determination method
  • A method for constructing the ULC characteristic map of the soil fritillaria medicinal material and its component content determination method
  • A method for constructing the ULC characteristic map of the soil fritillaria medicinal material and its component content determination method

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Experimental program
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Effect test

Embodiment 1

[0042] Construction Method of UPLC Characteristic Map of Soil Fritillaria Medicinal Material

[0043] 1. The chromatographic conditions for ultra-high performance liquid chromatography analysis are as follows: use a WatersCortecs T3 chromatographic column with a specification of 2.1×100mm and 1.6 μm, use acetonitrile as mobile phase A, and use 0.1% phosphoric acid aqueous solution as mobile phase B to carry out gradient washing The column temperature is 30°C; the flow rate is 0.3ml / min; the detection wavelength is 203nm, and the injection volume is 1µl.

[0044] 2. Gradient elution conditions are: 0~4min, the volume fraction of mobile phase A changes from 5% to 11%, and the volume fraction of mobile phase B is 95%~89%; 4~7min, the volume fraction of mobile phase A changes 11%~20%, the volume fraction of mobile phase B changes to 89%~80%; 7~21min, the volume fraction of mobile phase A changes to 20%~28%, and the volume fraction of mobile phase B changes to 80%~ 72%; 21~30min, ...

Embodiment 2

[0066] Method for Determination of Component Content in Soil Fritillaria Medicinal Material

[0067] 1. The chromatographic conditions for ultra-high performance liquid chromatography analysis are as follows: use a WatersCortecs T3 chromatographic column with a specification of 2.1×100mm and 1.6 μm, use acetonitrile as mobile phase A, and use 0.1% phosphoric acid aqueous solution as mobile phase B to carry out gradient washing The column temperature is 30°C; the flow rate is 0.3ml / min; the detection wavelength is 203nm, and the injection volume is 1µl.

[0068] 2. Gradient elution conditions are: 0~4min, the volume fraction of mobile phase A changes from 5% to 11%, and the volume fraction of mobile phase B is 95%~89%; 4~7min, the volume fraction of mobile phase A changes 11%~20%, the volume fraction of mobile phase B changes to 89%~80%; 7~21min, the volume fraction of mobile phase A changes to 20%~28%, and the volume fraction of mobile phase B changes to 80%~ 72%; 21~30min, t...

Embodiment 3

[0103] Using UPLC characteristic chromatogram to identify the medicinal material of Fritillaria japonicus.

[0104] The soil fritillary medicinal material is a medicinal material purchased randomly in the market.

[0105] 1. The chromatographic conditions for ultra-high performance liquid chromatography analysis are as follows: use a WatersCortecs T3 chromatographic column with a specification of 2.1×100mm and 1.6 μm, use acetonitrile as mobile phase A, and use 0.1% phosphoric acid aqueous solution as mobile phase B to carry out gradient washing The column temperature is 30°C; the flow rate is 0.3ml / min; the detection wavelength is 203nm, and the injection volume is 1µl.

[0106] 2. Gradient elution conditions are: 0~4min, the volume fraction of mobile phase A changes from 5% to 11%, and the volume fraction of mobile phase B is 95%~89%; 4~7min, the volume fraction of mobile phase A changes 11%~20%, the volume fraction of mobile phase B changes to 89%~80%; 7~21min, the volume ...

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Abstract

The present invention relates to a method for constructing the UPLC characteristic map of the soil fritillary medicinal material and a method for determining the component content thereof. The method for determining the component content of the soil fritillary medicinal material comprises the following steps: (1) absorbing the reference substance solution and the to-be-tested solution respectively The sample solution of Tuifumu medicinal material was injected into the ultra-high performance liquid chromatograph, and the corresponding peak area was measured; (2) The content of Tuifumoside A was calculated by the external standard method; B. The content of Tuimoside C. In the present invention, the peak area is measured by constructing the UPLC characteristic map of the medicinal material Fritillaria chinensis, and the content of fritillin A is calculated by the external standard method, and then the contents of fritillin B and C are calculated by one test and multiple evaluations. , fundamentally quickly and accurately carry out quantitative and qualitative analysis of Soil Fritillaria, which has certain characteristics. The method described in the present invention is fast, stable and specific, and has a stable baseline, providing scientific basis for the quality control of Soil Fritillaria. new method.

Description

technical field [0001] The invention belongs to the field of identification of traditional Chinese medicines, and in particular relates to a method for constructing a UPLC characteristic map of a soil fritillary medicinal material and a method for determining its component content. Background technique [0002] Fritillaria is commonly known as wild melon, also known as false fritillary, grass fritillary, ground bitter gall, etc. It was originally recorded in "Supplements to Compendium of Materia Medica" compiled by Zhao in the Qing Dynasty. The 2015 edition of "Chinese Pharmacopoeia" stipulates that fritillaria is calabash The dry tubers of the family plant Fritillaria are widely distributed in Hebei, Shanxi, Shandong, Henan, Hubei, Hunan and other places. It is reported in the literature that a variety of saponins, sterols and their glycosides, esters, alkaloids, flavonoids and other components have been isolated from Fritillaria, among which saponins are the main active co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/86
CPCG01N30/06G01N30/34G01N30/8679G01N30/8686G01N30/88G01N2030/047G01N2030/884
Inventor 黄敏烨李振雨童培珍曹斯琼潘礼业孙冬梅陈向东魏梅程学仁
Owner GUANGDONG YIFANG PHARMA
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