Triple PCR primers, kit and method for simultaneously detecting vibrio parahaemolyticus of three genotypes

Abstract

The invention discloses triple PCR primers and a kit for simultaneously detecting vibrio parahaemolyticus of three genotypes. The triple PCR primers comprise a primer TDH_F, a primer TDH_R, a primer TRH_F, a primer TRH_R, a primer pVA1_F and a primer pVA1_R. The kit using the primers can be used for detecting whether the vibrio parahaemolyticus contains a tdh gene, a trh gene or a pVA1 plasmid, and is strong in specificity and high in accuracy.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to triple PCR primers, a kit and a detection method for simultaneously detecting three genotypes of vibrio parahaemolyticus. Background technique [0002] Vibrio parahemolyticus (Vibrio parahemolyticus) is a Gram-negative bacillus with arc-shaped, rod-shaped, filamentous and other shapes without spores. At present, the PCR methods used to detect the bacteria mainly include arbitrary primer PCR (arbitrarilyprimedPCR, Ap-PCR), conventional PCR, multiplex PCR (multiplex-PCR), real-time PCR (Real-timePCR). The molecular biology method of bacterial identification usually uses the detection of bacterial 16S rRNA gene, but the difference in 16S rRNA gene between species with close relatives is small, which often leads to the failure of interspecies identification. KWOK et al. analyzed the partial heat shock protein HSP60 gene sequence of 29 marine Vibrio strain...

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Problems solved by technology

[0004] At present, molecular biology methods based on the tlh gene, tdh gene, and trh gene of Vibrio parahaemolyticus are relatively mature. However, since pVA1 is a plasmid with special toxicity confirmed by research in recent years, the current international There is no detection method that can simultaneously detect whether Vibrio parahaemolyticus strains contain tdh gene, trh gene, or pVA1 plasmid

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