Manufacturing method of microgel stent based on flexible mold capillary folding technology

A flexible mold and manufacturing method technology, applied in biochemical equipment and methods, cell culture supports/coatings, microorganisms, etc., can solve the problems of difficult to form three-dimensional microgel scaffolds, difficult to control scaffold morphology and other problems

Active Publication Date: 2020-04-24
XI AN JIAOTONG UNIV
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Problems solved by technology

However, these methods are difficult to control the morphology of scaffolds. For example, microfluidic technology can only prepare spherical or nearly spherical scaffolds. Photolithography and traditional mold methods can only prepare scaffolds with columnar structures (the cross-sectional shape of the columnar body can be adjusted), which is difficult. Microgel Scaffolds with Shaped Three-Dimensional Topography

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  • Manufacturing method of microgel stent based on flexible mold capillary folding technology
  • Manufacturing method of microgel stent based on flexible mold capillary folding technology
  • Manufacturing method of microgel stent based on flexible mold capillary folding technology

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0022] A method for manufacturing a microgel support based on flexible mold capillary folding technology, comprising the following steps:

[0023] 1) Design of the flexible mold: In order to prepare microgel scaffolds with different shapes, the shape of the flexible mold is optimally designed using topological knowledge. The flexible mold includes a flexible substrate, and one side of the flexible substrate is provided with periodic grabbing Structure; expanding the outer surface of the designed microgel scaffold to a two-dimensional plane is the shape of the flexible mold;

[0024] 2) Preparation of flexible mold:

[0025] 2.1) Spin-coat the first layer of photoresist 2 on silicon wafer or glass wafer 1, its thickness h 1 It is in the micron scale, and then pre-baked on a hot plate; through the top ultraviolet light 4, it is exposed through the fir...

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Abstract

A manufacturing method of a microgel stent based on a flexible mold capillary folding technology comprises the following steps: firstly, a flexible mold is designed, then the flexible mold is prepared, a microgel stent is prepared, and a grabbing structure of the flexible mold is in contact with the liquid level of hydrogel so that the hydrogel will wet the surface of the grabbing structure and capillary force is automatically generated at a contact line position; subsequently, the flexible mold is gradually pulled away from the liquid level of the hydrogel, the grabbing structure is graduallybent and deformed until the grabbing structure is finally closed under the action of capillary force, the hydrogel is wrapped in a formed cavity, and then the hydrogel is cross-linked and cured; andfinally, demolding of the microgel stent is conducted, the flexible mold and the hydrogel are immersed in water, water molecules infiltrate the interface of the grabbing structure and the hydrogel anddestroy the binding force between the grabbing structure and the hydrogel, the grabbing structure is automatically opened, and the hydrogel automatically falls off. According to the invention, controllable manufacturing of micron-scale hydrogel stents with different morphologies can be realized.

Description

technical field [0001] The invention belongs to the technical field of biomanufacturing, and in particular relates to a method for manufacturing a microgel support based on a flexible mold capillary folding technology. Background technique [0002] Micron-scale bioscaffolds are important tools for three-dimensional cell culture and construction of three-dimensional tissue models in vitro. Cell culture using micron-scale bioscaffolds (typically hundreds of microns) can mimic the basic functions of human organ units in vitro. Its advantage lies in the small size of the formed micro-tissues, and the transmission of nutrients and the discharge of metabolites can be realized by means of diffusion. At present, the most commonly used material for micron-scale biological scaffolds is hydrogel (ie, microgel scaffold). The main advantage of hydrogel materials is that they have good biocompatibility and are conducive to cell adhesion; at the same time, hydrogels have suitable pore siz...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/38A61L27/52C12N5/00
CPCA61L27/18A61L27/38A61L27/52C12N5/00C12N2533/00C08L83/04
Inventor杨清振吕雪蒙徐峰张辉刘妍
OwnerXI AN JIAOTONG UNIV