G-6-PD detection kit free from anemia influences and detection method thereof

A G-6-PD, kit technology, applied in the direction of material analysis, measuring device, instrument, etc. by observing the effect on chemical indicator, can solve the problem of inaccurate test results, achieve flexible and convenient operation, correct measurement error, the effect of improving test accuracy

Active Publication Date: 2020-04-24
QINGDAO HIGHTOP BIOTECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of inaccurate detection results of anemia patients in the prior art, and propose a G-6-PD detection kit that can conveniently measure the degree of anemia of the subject and quickly confirm the amount of sample added and its

Method used

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  • G-6-PD detection kit free from anemia influences and detection method thereof
  • G-6-PD detection kit free from anemia influences and detection method thereof

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Embodiment

[0027] Preparation of G-6-PD test paper: soak the reaction film in the G-6-PD detection solution to saturation, cut it into shape after drying to obtain the G-6-PD detection block, and fix the G-6-PD detection block Paste on one end of the carrier to make a test paper;

[0028] Preparation of G-6-PD detection solution: 0.20mol / L Tris-HCl buffer solution (pH7.4); 0.15% phenazine dimethyl sulfate (M-PMS); 0.75% nitro blue tetrazolium (NBT); Glucose-6-phosphate disodium (G6P-Na 2 ); 12mmol / L Coenzyme II (NADP); 0.6mol / L MgCl 2 Mix in proportion to obtain G-6-PD detection solution.

[0029] Preparation of hemoglobin test paper: soak the reaction membrane in the hemoglobin detection solution to saturation, cut it into shape after drying to obtain a hemoglobin detection block, fix and paste the hemoglobin detection block on one end of the carrier, and prepare the test paper;

[0030] Preparation of hemoglobin detection solution: 0.1mol / L PBS buffer solution (pH6.5), 0.15% sodium ...

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Abstract

The invention belongs to the technical field of biochemical detection, and particularly relates to a G-6-PD detection kit free from anemia influences and a detection method thereof. The kit comprisesa sample detection device, hemolytic agent dry powder, hemoglobin test paper, a sample adding colorimetric card, G-6-PD test paper and a detection colorimetric card, wherein the sample detection device comprises a sample adding pipe, a first clamping groove and a second clamping groove. According to the kit and the detection method thereof provided by the invention, the anemia degree of a subjectcan be conveniently determined, the sample adding amount can be rapidly determined, the method is used for screening detection of erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) deficiency, thesample adding amount can be compensated according to hemoglobin test results, the test method is small in blood consumption and flexible and convenient in test, and meanwhile, the accuracy of the detection results is improved.

Description

technical field [0001] The invention belongs to the technical field of detection of in vitro diagnostic reagents, and in particular relates to a G-6-PD detection kit and a detection method thereof which are not affected by anemia. Background technique [0002] Anemia is a clinical syndrome in which the volume of peripheral blood red blood cells is lower than normal due to various reasons. (Hb) is most important. Chinese hematologists believe that in China's sea level areas, adult male Hb<120g / L (12.0g / dl), adult female Hb<110g / L (11.0g / dl), pregnant woman's Hb<120g / L, have anemia. Erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) is derived from erythrocytes, catalyzes glucose-6-phosphate, and the NAD-PH generated is the coenzyme of glutathione reductase, reduced glutathione (GSH ) is a necessary condition for maintaining the stability of hemoglobin and the integrity of the red blood cell membrane. In the absence of red blood cell G-6-PD, the function of th...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/01
CPCG01N21/78G01N21/01
Inventor 杨帆杨勤德邓旭崔欣顾晓云宋金玲刘秀艳
Owner QINGDAO HIGHTOP BIOTECH
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