G-6-PD detection kit free from anemia influences and detection method thereof

Abstract

The invention belongs to the technical field of biochemical detection, and particularly relates to a G-6-PD detection kit free from anemia influences and a detection method thereof. The kit comprisesa sample detection device, hemolytic agent dry powder, hemoglobin test paper, a sample adding colorimetric card, G-6-PD test paper and a detection colorimetric card, wherein the sample detection device comprises a sample adding pipe, a first clamping groove and a second clamping groove. According to the kit and the detection method thereof provided by the invention, the anemia degree of a subjectcan be conveniently determined, the sample adding amount can be rapidly determined, the method is used for screening detection of erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) deficiency, thesample adding amount can be compensated according to hemoglobin test results, the test method is small in blood consumption and flexible and convenient in test, and meanwhile, the accuracy of the detection results is improved.

Description

technical field [0001] The invention belongs to the technical field of detection of in vitro diagnostic reagents, and in particular relates to a G-6-PD detection kit and a detection method thereof which are not affected by anemia. Background technique [0002] Anemia is a clinical syndrome in which the volume of peripheral blood red blood cells is lower than normal due to various reasons. (Hb) is most important. Chinese hematologists believe that in China's sea level areas, adult male Hb<120g / L (12.0g / dl), adult female Hb<110g / L (11.0g / dl), pregnant woman's Hb<120g / L, have anemia. Erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) is derived from erythrocytes, catalyzes glucose-6-phosphate, and the NAD-PH generated is the coenzyme of glutathione reductase, reduced glutathione (GSH ) is a necessary condition for maintaining the stability of hemoglobin and the integrity of the red blood cell membrane. In the absence of red blood cell G-6-PD, the function of th...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of inaccurate detection results of anemia patients in the prior art, and propose a G-6-PD detection kit that can conveniently measure the degree of anemia of the subject and quickly confirm the amount of sample added and its The detection method is used for the screening detection of red blood cell glucose-6-phosphate dehydrogenase (G-6-PD) deficiency. The detection kit can compensate the sample amount according to the hemoglobin test result. Flexible and convenient, while improving the accuracy of test results

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