Method for constructing type III collagen photoelectrochemical sensor based on rhenium disulfide nanosheet and application

A technology of rhenium disulfide and collagen, applied in the direction of electrochemical variables of materials, nanotechnology for sensing, nanotechnology for materials and surface science, etc., can solve the problem of time-consuming, non-concentrated ultrasonic energy, and peeling effect It has the advantages of simple preparation method, concentrated ultrasonic energy, and shortened peeling time.

Active Publication Date: 2020-04-24
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the peeling method of rhenium disulfide nanosheets is mainly to use water bath ultrasonic peeling in a single solvent. Due to the lack of concentration of ultrasonic energy, the whole peeling process takes a long time and the peeling effect is not good.

Method used

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  • Method for constructing type III collagen photoelectrochemical sensor based on rhenium disulfide nanosheet and application
  • Method for constructing type III collagen photoelectrochemical sensor based on rhenium disulfide nanosheet and application
  • Method for constructing type III collagen photoelectrochemical sensor based on rhenium disulfide nanosheet and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A kind of 1-butyl-3-dodecyl imidazolium bromide ionic liquid, its preparation method is as follows:

[0060] (1) Take by weighing sodium hydride (0.04mol, 0.96g) and add in the 120mL acetonitrile solution containing imidazole (0.02mol, 1.36g) several times in small amounts, form the acetonitrile suspension of white imidazole sodium after 3h of ice-bath reaction, cool to Standby at room temperature;

[0061] (2) Add bromododecane (0.01mol, 2.42g) to the acetonitrile suspension of imidazole sodium obtained in step (1), raise the temperature to 65° C., stir overnight, point the plate to monitor the reaction process, and pump out after the reaction is completed. Filter, remove insoluble impurities, and remove acetonitrile by rotary evaporation to obtain the crude product, then gradient elution through a silica gel column, the eluent is (CH 2 Cl 2 : MeOH=10:1), obtain light yellow oily liquid dodecyl imidazole;

[0062] (3) Dissolve dodecyl imidazole (0.01mol, 2.36g) and ...

Embodiment 2

[0065] A method for preparing rhenium disulfide nanosheets based on ionic liquid-assisted exfoliation, the steps are as follows:

[0066] (1) Take 0.1g of 1-butyl-3-dodecyl imidazolium bromide in a centrifuge tube, add 15mL of N-methylpyrrolidone to dissolve it completely, and then add 20mg of ReS 2 The powder is ultrasonicated with a probe ultrasonic machine, the probe ultrasonic treatment time is 3h, the power is 300W, the ultrasonic machine is set to work for 5s and pause for 2s, and the dispersed ReS 2 A solution of nanosheets;

[0067] (2) The ReS obtained in step (1) 2 The nanosheets were divided into centrifuge tubes, and centrifuged at 500rpm for 5min to remove blocky ReS 2 , the collection contains ReS 2 The supernatant of the nanosheets was centrifuged at 12,000rpm for 15min to remove the solvent, washed three times with deionized water and absolute ethanol alternately, and dried in vacuum to obtain ReS 2 Nanosheets.

[0068] figure 2 ReS prepared for this exa...

Embodiment 3

[0074] like Figure 7 As shown, a method for constructing type III collagen photoelectrochemical sensors based on rhenium disulfide nanosheets comprises the following steps:

[0075] S1. Glassy carbon electrode pretreatment: straight bare glassy carbon electrode (diameter 3mm), with 0.3μm Al 2 o 3 The suspension was polished, rinsed with distilled water, and then successively in HNO 3 , ethanol, double distilled water ultrasonic cleaning, dry for later use;

[0076] S2, with the ReS that embodiment 2 makes 2 Nanosheets dispersed in water to form 2mg·mL -1 For the dispersion solution, take 8 μL ReS 2 The dispersion liquid is drop-coated on the surface of the clean glassy carbon electrode obtained in step S1, and dried at room temperature to obtain ReS 2 / GCE modified electrode;

[0077] S3. Continue to drip-coat 6 μL of 10 μg / mL type III collagen antibody solution on the surface of the electrode prepared in step S2, dry it at room temperature, and use 0.01mol L -1 PBS b...

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Abstract

The invention discloses a method for constructing a type III collagen photoelectrochemical sensor based on a rhenium disulfide nanosheet and an application. The method comprises the following steps offirstly, preparing 1-butyl-3-dodecyl imidazole bromide salt ionic liquid; taking the ionic liquid as an auxiliary reagent; successfully manufacturing a rhenium disulfide two-dimensional lamellar nano-material by using probe ultrasonic stripping; and constructing the type III collagen photoelectrochemical sensor on the basis of a rhenium disulfide nano-sheet modified electrode and loading of a type III collagen antibody, and realizing specific detection of type III collagen by taking white light as an excitation light source. In the invention, the ionic liquid is used as a stripping agent to be inserted into or attached to a nano lamellar structure of rhenium disulfide so that rhenium disulfide nanosheet agglomeration can be effectively avoided, and stripping efficiency is improved; and rhenium disulfide belongs to a direct band gap material, and has a better photoelectric property than other transition metal disulphides so that high-selectivity and high-sensitivity analysis of the type III collagen is realized.

Description

technical field [0001] The invention belongs to the technical field of novel nano-functional materials and biosensing, and in particular relates to a method and application for constructing type III collagen photoelectrochemical sensors based on rhenium disulfide nanosheets. Background technique [0002] Abdominal aortic aneurysm (AAA) is a severe progressive degenerative disease characterized by dilation of the abdominal aorta, accompanied by degradation of the extracellular matrix of the arterial wall and the occurrence of chronic inflammation. The incidence rate of AAA among elderly men over 65 years old is as high as 8%, and the incidence rate of AAA is slightly higher than that of women. It is the 13th cause of death in my country. Smoking, advanced age and atherosclerosis have been proven to be the most important risk factors for AAA. Most AAA patients have no obvious clinical symptoms except occasional abdominal pain, and a small number of patients have ruptured tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N21/63B82Y15/00B82Y30/00B82Y40/00
CPCG01N27/308G01N27/3278G01N21/63B82Y15/00B82Y30/00B82Y40/00
Inventor 孙东黄雅娇张锦涛
Owner WENZHOU MEDICAL UNIV
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