Polyiodinated aromatic acid compound and application thereof in resisting adenovirus type 7

A compound and adenovirus technology, applied in antiviral agents, medical preparations containing active ingredients, organic chemistry, etc., can solve the problems of unreported inhibitory activity, achieve great clinical application prospects, reduce production, and inhibit replication and proliferation Effect

Active Publication Date: 2020-05-08
HUBEI UNIV OF TECH
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antiviral activity of iodocarboxylic acids, including the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polyiodinated aromatic acid compound and application thereof in resisting adenovirus type 7
  • Polyiodinated aromatic acid compound and application thereof in resisting adenovirus type 7
  • Polyiodinated aromatic acid compound and application thereof in resisting adenovirus type 7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] [Example 1] Research experiment on anti-ADV7 activity of polyiodoaromatic acid compounds L4~L7

[0030] The experimental situation is as follows:

[0031] 1. Test content:

[0032] Anti-ADV7 activity analysis of compounds: In the present invention, the anti-ADV7 activity of polyiodocarboxylic acids will be evaluated by combining cytopathic effect analysis and MTT assay cell survival rate detection method.

[0033] 2. Test method:

[0034] 2.1.1 Toxicity of compounds to host Hela cells

[0035] Hela cells were plated in 96-well plates at 37°C, 5% CO 2 After the monolayer was grown in the incubator, the cell culture solution was discarded, and the cell maintenance solution containing different concentrations of the test compound was added to continue the culture. After 48 hours, the cytotoxicity was visually observed under the microscope and recorded respectively, and the cell survival rate was determined by the MTT method. SPSS 11.5 software calculates the median tox...

Embodiment 2

[0046] [Example 2] The inhibitory effect test of polyiodoaromatic acid compounds L4, L5, L7 on the yield of ADV7 progeny virus

[0047] This embodiment has carried out in-depth research on the anti-ADV7 activity of polyiodocarboxylic acids (L4~L7), and implemented the inhibitory effect test of compounds L4, L5, and L7 on the production of ADV7 progeny virus, and the test conditions are as follows:

[0048] 1. Test content

[0049] After ADV7 infected Hela cells, the inhibitory effect of compounds L4, L5, and L7 on the production of ADV7 progeny virus was detected.

[0050] 2. Test method

[0051] Hela cells in the logarithmic growth phase were plated in 24-well plates, and 100TCID after the monolayer was overgrown 50 ADV7 infected cells, incubated at 37°C for 1.5h, removed the virus solution, washed three times with PBS, and added 200 μg / mL L4, 50 μg / mL L5, and 100 μg / mL L7 cell maintenance solution. After 48 hours, the cells and supernatant were collected and lysed by free...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a polyiodinated aromatic acid compound and an application thereof in resisting adenovirus type 7. Anti-ADV7 virus activity research experiments of polyiodinated carboxylic acidcomprising 3-amino-4-iodobenzoic acid (L4), 3,4-diiodobenzoic acid (L5), 3,5- diiodosalicylic acid (L6) and 2,3,5-triiodobenoic acid (L7) show that the compounds L4, L5 and L7 inhibit cytopathic effect (CPE) generated by ADV7 on host cells Hela, the cell survival rate is increased, the yield of filial generation viruses is reduced, and the compounds can be applied to preparation of anti-ADV7 virus drugs.

Description

technical field [0001] The invention relates to the technical field of antiviral drugs, and specifically refers to a polyiodoaromatic acid compound and its application in anti-adenovirus type 7. Background technique [0002] Adenoviruses (adenoviruses, ADVs) are a group of widely distributed DNA viruses. There are 52 known species of somatic adenoviruses, named ADV1-ADV52 respectively, divided into two genera, with a total of more than 100 serotypes. About one-third of the known serotypes of human adenoviruses are associated with human disease. Adenoviruses can invade various tissues and organs such as the respiratory tract, conjunctiva, gastrointestinal tract, and urinary tract. The main infection is the respiratory tract. In severe cases, it can cause pneumonia in children and accumulate in various systems of the body. Adenoviral pneumonia caused by adenovirus infection is one of the most serious types of pneumonia in children. The adenovirus types associated with respi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C229/60C07C63/70C07C65/05A61K31/196A61K31/192A61P31/20
CPCC07C229/60C07C63/70C07C65/05A61P31/20Y02A50/30
Inventor 魏艳红王海杰胡康洪李妮王龙胜朱茂春
Owner HUBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap