Strain Anaerostipes sp B2131 and application thereof in inflammatory intestinal diseases

A technology of inflammatory bowel disease, cgmccno.1.5295, applied in the field of microorganisms, can solve the problem of lack of Lachnospiraceae strains and other problems, achieve significant application value, have no toxic side effects, and improve the effect of intestinal flora

Active Publication Date: 2020-05-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But up to now, there is still a lack of Lachnospiraceae strains clinically used for the treatment of IBD, so it is of great significance to find and develop Lachnospira strains

Method used

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  • Strain Anaerostipes sp B2131 and application thereof in inflammatory intestinal diseases
  • Strain Anaerostipes sp B2131 and application thereof in inflammatory intestinal diseases
  • Strain Anaerostipes sp B2131 and application thereof in inflammatory intestinal diseases

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0040] Reagents, materials and equipment

[0041]

[0042]

Embodiment 1

[0043] Embodiment 1, the isolation, cultivation and identification of Anaerostipes sp.B2131 bacteria

[0044] (1) Conditioned medium preparation

[0045] Pre-deoxygenated liquid BHI medium (containing 5% bovine serum and 0.1% cysteine): Weigh 37 g of brain heart infusion (BD company) into a 1 L Erlenmeyer flask, add 1 g of cysteine ​​and double distilled Dilute the water to 1000ml, stir to dissolve, and then sterilize in an autoclave at 121°C for 15min; Shake evenly to obtain pre-deoxygenated liquid BHI medium, and then divide into 50ml centrifuge tubes.

[0046] Pre-deoxygenated solid BHI medium (containing 5% bovine serum, 0.1% cysteine ​​and 1 mg / L aztreonam): take 37 g of brain heart infusion (BD company) in a conical flask with a capacity of 1 L, add 1g cysteine, 15g agar powder and double-distilled water to a volume of 1000ml, stir to dissolve, and then sterilize in an autoclave at 121°C for 15min; after the sterilization is completed, put the medium into an anaerobic ...

Embodiment 2

[0063] Example 2, Abundance Detection of Anaerostipes sp. Bacterial Content in IBD Patients and Healthy Controls

[0064] (1) Fecal flora DNA extraction

[0065] Stool samples from IBD patients were provided by inpatients in the Inflammatory Bowel Disease Center of Run Run Shaw Hospital Affiliated to Zhejiang University. After collection, the samples were frozen and stored in a -20°C refrigerator in time, and transported to the laboratory with dry ice in a -80°C refrigerator within 2 days. The fecal samples of healthy people were provided by healthy people for physical examination, and they were taken back to the laboratory within 5 hours and stored in a -80°C refrigerator. When extracting fecal DNA, take out the sample from -80°C, and take 100-150 mg of feces with a medicine spoon into a 1.5ml centrifuge tube without thawing; then, use the fecal flora DNA extraction kit (Qiagen) to extract the total DNA of fecal flora , and then use the Nanodrop 2000 UV micro-spectrophotomet...

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Abstract

The invention discloses a strain Anaerostipes sp B2131. The strain Anaerostipes sp B2131 has a collection number of CGMCC No. 1. 5295. The invention further simultaneously discloses application of thestrain Anaerostipes sp B2131 in preparation of drugs for preventing and / or treating inflammatory intestinal diseases. The enteric canal separated bacterium disclosed by the invention can be applied to products such as pharmaceutical compositions, foods, healthcare products and food additives as a probiotics preparation and is used for regulating a stable state of enteric canal florae and preventing and treating occurrence of enteric canal diseases such as the inflammatory intestinal diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of Anaerostipes sp. bacteria in preventing and / or treating inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease (Inflammatory Bowel Disease, IBD) is a group of diseases characterized by chronic inflammation of the digestive tract, mainly including ulcerative colitis subtypes (Ulcerative Colitis, UC) and Crohn's Disease subtypes (Crohn's Disease, CD ). The clinical manifestations of IBD are mainly persistent diarrhea, abdominal pain, rectal bleeding / blood in the stool, weight loss, etc., which seriously affect the quality of life of patients and are prone to relapse. It is considered a lifelong disease. As of 2016, the incidence of UC and CD in European and American countries exceeded 0.3% (incidence population / national population), among which the incidence of UC was the highest in Norway, reaching 0.505%, while ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/741A61P1/00A23L33/135C12R1/01
CPCA23V2002/00A61K35/741A23L33/135A61P1/00C12N1/205C12R2001/01A23V2200/32A23V2200/30
Inventor 孙德森许正平盛静浩白荣盘
Owner ZHEJIANG UNIV
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