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Liquid composite mycorrhiza bacterial agent for promoting growth of blueberry seedlings, and preparation method thereof

A compound inoculant and compound bacteria technology, applied in the directions of plant growth regulators, botanical equipment and methods, biocides, etc., can solve the problems of lack of symbiotic mycorrhiza, lack of symbiotic mycorrhizal fungi, affecting the growth of blueberries, etc., and achieve control Production cost, saving operation time and required materials, significant effect of growth promotion

Inactive Publication Date: 2020-05-22
浙江省大盘山国家级自然保护区管理局
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Problems solved by technology

However, in the actual production of blueberries, on the one hand, blueberry seedlings are mostly aseptic seedlings obtained by tissue culture; on the other hand, in order to prevent pests and diseases, the planting soil or cultivation substrate is often disinfected, so that the blueberry root system lacks the necessary symbiotic mycorrhizal fungi. greatly affected the growth of blueberries
[0004] In order to make up for the deficiencies of chemical fertilizers and organic fertilizers, in view of the fact that blueberries are mycorrhizal-dependent plants and lack symbiotic mycorrhizal under cultivation conditions, we need a compound mycorrhizal agent to improve the growth of blueberries

Method used

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  • Liquid composite mycorrhiza bacterial agent for promoting growth of blueberry seedlings, and preparation method thereof

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Embodiment Construction

[0029] A liquid compound mycorrhizal agent for promoting the growth of blueberry seedlings and a preparation method thereof, comprising the following steps:

[0030] (1) Single strain expansion and cultivation:

[0031] Strain activation: In the ultra-clean workbench, pick a small amount of hyphae from the preserved strains T1, T2, and T3 and inoculate them on the potato dextrose agar medium plate, and culture them in the dark at 25 °C for 2 to 3 days, and then again Transfer to a new potato dextrose agar medium plate and cultivate for 2 to 3 days;

[0032] Preparation of shaker fermentation medium: wash potatoes, peel them, cut them into small pieces, weigh 80 ~ 120 g, add 700 ~ 800 ml of distilled water, pour them into a blender and crush them for 2 ~ 3 minutes to form a slurry, then add Glucose 20 g and penicillin 0.1 g were fully dissolved, and finally the volume of distilled water was adjusted to 1000 ml, and divided into 250 ml conical flasks, and 150 ml was poured into...

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Abstract

The invention discloses a liquid composite mycorrhizal bacterial agent for promoting growth of blueberry seedlings, and a preparation method thereof. The preparation method comprises the following steps: (1) propagating culture of a single strain; and (2) preparation of a complex bacterial agent, wherein the strains of the mycorrhizal bacterial agent disclosed by the invention is three mycorrhizalbacteria T1, T2 and T3 separated from the root system of Rhododendron simsii. According to the invention, after the bacterial agent is applied, the T1, T2 and T3 liquid composite bacterial agent hasthe most significant effect of promoting the growth of blueberry seedlings; and the preparation method saves the operation time and required materials, reduces waste discharge, improves the operationefficiency in production practice, and controls the batch production cost.

Description

technical field [0001] The invention relates to a liquid compound mycorrhizal agent for promoting the growth of blueberry seedlings and a preparation method thereof. Background technique [0002] Blueberry (Vaccinium corymbosum) belongs to the genus Vaccinium of the family Rhododendraceae. The pulp is delicate, sweet and sour, and rich in anthocyanins and other nutrients. It is a world-renowned economic berry plant. In recent years, the cultivation and production of blueberries in my country has developed rapidly. As of 2017, there have been large-scale plantings in nearly 30 provinces, municipalities and autonomous regions across the country. How to promote the growth of blueberries to improve economic benefits is a fundamental problem in the large-scale planting of blueberries. Although the application of chemical or organic fertilizers to blueberries can help the growth of blueberries, chemical fertilizers can easily lead to soil degradation and pollution and hinder the ...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01P3/00
Inventor 王盼刘亚陈江芳周钰鸿倪喜中陈子林金烽张国安包金亮
Owner 浙江省大盘山国家级自然保护区管理局
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