Liquid composite mycorrhiza bacterial agent for promoting growth of blueberry seedlings, and preparation method thereof
A compound inoculant and compound bacteria technology, applied in the directions of plant growth regulators, botanical equipment and methods, biocides, etc., can solve the problems of lack of symbiotic mycorrhiza, lack of symbiotic mycorrhizal fungi, affecting the growth of blueberries, etc., and achieve control Production cost, saving operation time and required materials, significant effect of growth promotion
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[0029] A liquid compound mycorrhizal agent for promoting the growth of blueberry seedlings and a preparation method thereof, comprising the following steps:
[0030] (1) Single strain expansion and cultivation:
[0031] Strain activation: In the ultra-clean workbench, pick a small amount of hyphae from the preserved strains T1, T2, and T3 and inoculate them on the potato dextrose agar medium plate, and culture them in the dark at 25 °C for 2 to 3 days, and then again Transfer to a new potato dextrose agar medium plate and cultivate for 2 to 3 days;
[0032] Preparation of shaker fermentation medium: wash potatoes, peel them, cut them into small pieces, weigh 80 ~ 120 g, add 700 ~ 800 ml of distilled water, pour them into a blender and crush them for 2 ~ 3 minutes to form a slurry, then add Glucose 20 g and penicillin 0.1 g were fully dissolved, and finally the volume of distilled water was adjusted to 1000 ml, and divided into 250 ml conical flasks, and 150 ml was poured into...
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