Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Analysis method for succession of microbial communities during tobacco mellowing

A technology of microbial community and analysis method, which is applied in the field of analysis of microbial community succession in the process of tobacco leaf alcoholization, can solve the problems of lack of research and cognition of microbial community structure and dynamic changes, achieve reduced fermentation costs, broad application prospects, The effect of improving the quality of tobacco leaves

Inactive Publication Date: 2020-05-22
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of research and knowledge on the structure and dynamic changes of microbial flora during the alcoholization of tobacco leaves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Analysis method for succession of microbial communities during tobacco mellowing
  • Analysis method for succession of microbial communities during tobacco mellowing
  • Analysis method for succession of microbial communities during tobacco mellowing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Analysis method of microbial community succession in tobacco leaf alcoholization process.

[0029] 1) Sampling: Sampling the naturally aged tobacco leaf samples at the same time intervals from the beginning of aging. In this example, the tobacco leaf samples were Hongdahe K326 tobacco leaf variety from Yunnan, and the total 24 months from the beginning of natural aging to the end of aging were taken every 3 months, a total of 14 samples. The sample collection adopts the principle of random sampling. According to the sampling sequence, the marked sample numbers are: H1, H2, H3, H4, H5, H6, H7, K1, K2, K3, K4, K5, K6, K7, of which H1~H7 They are all samples of Dababa tobacco leaves from Yunnan, and K1-K7 are all samples of K326 tobacco leaves.

[0030] 2) Construction of gene library: Genomic DNA of samples collected at each time period was extracted, and 16SrRNA gene library and internal intertranscriptional region (ITS) gene library were respectively constru...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an analysis method for succession of microbial communities during tobacco mellowing, and belongs to the technical field of environmental microbes. In order to define microbialpopulation structures and dynamic changes thereof and predict microbial gene functions during tobacco mellowing, the invention provides the analysis method for the succession of the microbial communities during tobacco mellowing, and the analysis method comprises the following steps of (1) performing sampling: taking naturally mellowed tobacco samples at the beginning of mellowing at intervals ofthe same time; (2) constructing gene libraries: extracting genomic DNA of the samples collected in each time period, and constructing a 16S ribosomal RNA (16S rRNA) gene library and an internal transcribed spacer region gene library; and (3) analyzing data of each gene library constructed in the step (2) to obtain the rule of the succession of the microbial communities during tobacco mellowing. The analysis method can be used for giving guidance on artificially mellowing tobacco and establishing the microbial communities.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to an analysis method for microbial community succession in the alcoholization process of tobacco leaves. Background technique [0002] Tobacco leaf raw materials have defects of varying degrees in the internal quality after harvesting, such as heavy green taste, strong irritation, rough smoke, smoke aroma covered by miscellaneous gas, and bitter, spicy, astringent and other bad feelings. It cannot be directly used to make cigarettes, and must be properly alcoholized. Tobacco leaf fermentation methods mainly include artificial alcoholization and natural alcoholization. Natural aging is a slow natural fermentation process. Artificial alcoholization refers to the artificial control of temperature and relative humidity to accelerate the growth and changes of microorganisms on tobacco leaves, enzymatic reactions and chemical changes between other subst...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12Q1/689C12Q1/04A24B15/20
CPCA24B15/20C12Q1/689C12Q1/6895C12Q2600/158
Inventor 张海波王帆咸漠门潇张鸽陈国强石东祥
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com