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Methods for chromatography resin slurry determination

A resin and slurry technology, applied in the field of slurry concentration of packed chromatographic columns, can solve the problems of increasing method uncertainty and spending a lot of time

Pending Publication Date: 2020-05-26
REGENERON PHARM INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gravity settling is considered more consistent but can take a significant amount of time to complete and correction factors may need to be empirically determined and applied, adding to method uncertainty

Method used

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  • Methods for chromatography resin slurry determination
  • Methods for chromatography resin slurry determination
  • Methods for chromatography resin slurry determination

Examples

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example 1

[0040] According to the invention a method for determining the concentration of a stock is carried out. The method was performed with a 1 cm x 25 cm chromatography SNAP column. A KD Scientific syringe pump was used to provide a controlled flow rate to consolidate the resin samples. The following resins were tested: MabSelect SuRe TM , 50HQ and Capto TM Phenyl.

[0041] Table 1 shows the results of the method performed according to the invention.

[0042] Table 1: Results of the inventive method

[0043]

[0044]

[0045] As can be seen from the data in Table 1, the method of the invention demonstrated consistent and reproducible results with low standard deviations between repeated measurements. The slurry concentrations obtained according to the present invention result in an average bed height that differs from the target bed height by 0.4 cm or less. A low standard deviation indicates that the results are highly reproducible.

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Abstract

The present invention relates to methods for determining a slurry concentration for a slurry of a chromatography resin during a column packing. The present invention provides a more accurate and consistent method for determining a slurry concentration for packing a chromatography column. The method of the present invention utilizes an automated pump to provide a controlled flow rate to consolidatea resin sample. Based on the consolidated resin, a slurry concentration can be determined. The determined slurry concentration can be utilized to pack the resin in a chromatography column with highlyaccurate bed heights.

Description

technical field [0001] The present invention relates to a method for resin slurry determination of chromatography resin slurries used during column packing. More specifically, the present invention provides a more accurate and consistent method for determining the concentration of a slurry used to fill a chromatography column, eg, by means of axial compression. [0002] Background of the invention [0003] Resin slurry determination is a method commonly used to determine the amount of chromatographic media present in a water-based slurry. Resin slurry percentage or slurry concentration is used in calculations during column packing operations to determine how much aqueous slurry should be placed in the column to ensure proper operation and target bed height. The slurry concentration must be determined and entered into an automated chromatographically packed column (e.g. column) to consistently fill to the correct height and fill factor. In fact, such as Automatic chromat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/20G01N30/56G01F23/292
CPCB01D15/206G01F23/00G01N30/32G01N30/56G01N30/88G01N2030/324G01N2030/326G01N2030/565G01N2030/8804
Inventor N·毛E·雪莉J·加莱亚R·冯T·迪萨纳亚克B·希林S·卡弗
Owner REGENERON PHARM INC