Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for simultaneous detection of ofloxacin and sarafloxacin in water body

A technology of ofloxacin and sarafloxacin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the influence of impurities cannot be ruled out, and the separation time is long, so as to achieve short detection time, strong selectivity, and high usage little effect

Inactive Publication Date: 2013-10-09
CHINESE RES ACAD OF ENVIRONMENTAL SCI
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography has high automation and good reproducibility, but for environmental samples with complex components, the separation time is long, and the influence of impurities cannot be ruled out, and the target compound can be accurately screened out for quantification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneous detection of ofloxacin and sarafloxacin in water body
  • Method for simultaneous detection of ofloxacin and sarafloxacin in water body
  • Method for simultaneous detection of ofloxacin and sarafloxacin in water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: recovery experiment

[0049]Take 1000ml of ultrapure water and 1000ml of actual water sample in a brown glass bottle, add 1ml of 100μg / L two kinds of trace fluoroquinolone antibiotics mixed standard stock solution to each bottle of water sample, mix well and adjust the pH of the water sample to about 3.0 with acid , using GF / F (Whatman, 0.7 μm) glass fiber membrane to filter to obtain the pretreated water sample. Use 6mL chromatographically pure acetone, 6mL chromatographically pure methanol, and 6mL 5mM 0.1% formic acid aqueous solution to activate the HLB (Waters, 500mg, 6cc) solid phase extraction column, and finally add 5mL ultrapure water to keep the resin in the small column in an activated state. Take 500~ Pass 1000mL water sample through the column, control the flow rate at 3.0-5.0mL / min; after the enrichment is completed, dry the HLB solid phase extraction column under nitrogen protection for 20min, then use 10mL eluent to elute the HLB column, co...

Embodiment 2

[0054] Example 2: The recovery results of water samples from Beijing Olympic Park.

[0055] Take 1000ml of river water samples from Beijing Olympic Park in a brown glass bottle, and carry out the standard addition experiment according to the method in Example 1, while taking 1000ml of river water samples without adding standards, as a blank control.

[0056] The results of the spiked water samples were brought into the standard curve equation, and the concentrations of the three sulfonamide antibiotics in the water samples to be tested were finally obtained by calculation. The recovery rate was calculated, and the results are shown in Table 2.

Embodiment 3

[0057] Example 3: Measurement results of actual water samples in Wangyanggou, Shijiazhuang, Hebei.

[0058] Take 1000ml of Shijiazhuang Wangyanggou river water sample in a brown glass bottle, and carry out the standard addition experiment according to the method in Example 1.

[0059] The concentrations of the two FQs in the samples were determined by high performance liquid chromatography-tandem mass spectrometry. The calculation results show that the two FQs detected in the water samples of Wangyanggou River in Shijiazhuang, that is, ofloxacin and sarafloxacin, are 4.36 and 3.916 ng / L.

[0060] Table 1

[0061]

[0062] Table 2

[0063]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for simultaneous detection of ofloxacin and sarafloxacin in water body. The method mainly comprises the following steps of adjusting a pH value of a water sample to 2-4 by an acid; filtering the water sample to obtain a pretreated water sample; activating an HLB solid-phase extraction column by acetone, methanol and a formic acid aqueous solution containing ammonium acetate successively; adding ultrapure water to keep a resin in the HLB solid phase extraction column in an activated state; passing the water sample through the column; drying the HLB solid-phase extraction column under the protection of nitrogen after enrichment is finished; eluting with an eluent; collecting the eluent; drying under a nitrogen flow; adding acetonitrile to dissolve residual materials for detection; and detecting the concentrations of ofloxacin and sarafloxacin quantitatively. The method is advantageous in that the pretreatment of the water sample is environment-friendly; and the method can be operated easily, has high enrichment coefficient, and can analyze the content of two trace fluoroquinolone antibiotics in a water environment rapidly and accurately.

Description

technical field [0001] The invention belongs to the technical field of detection of water environmental pollutants, in particular to a method for detecting the content of two trace fluoroquinolone antibiotics in water, more specifically for trace amounts of ofloxacin and sarafloxacin in surface water detection method. Background technique [0002] Fluoroquinolones (FQs) antibiotic drugs are a class of artificially synthesized antibacterial drugs, which have good antibacterial effects on Gram-negative bacteria and Gram-positive bacteria, because of their strong tissue penetration, low toxicity, and more It is widely used because of high drug tolerance and longer half-life. Fluoroquinolone antibiotics include norfloxacin (NOR), ciprofloxacin (CIP), ofloxacin (OFL), lomefloxacin (LOM), difloxacin (DIF), sarafloxacin (SAR) etc. Although some of these compounds can be absorbed by humans and livestock, and removed in sewage treatment processes, a large number of compounds are st...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88
Inventor 席北斗卢义许其功丁京涛高如泰张慧梁琼张媛
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com