Preparation method of stem cell hair growth promoting factors capable of protecting and inducing regeneration of hair follicles
A stem cell germinal factor and stem cell technology, applied in the field of stem cell germinal factor preparation, can solve the problems of increasing the risk of cell contamination and cell damage, cumbersome UVB irradiation operation, etc., to shorten the culture period, reduce the risk of pollution, and increase the yield and productivity effects
Inactive Publication Date: 2020-06-05
陕西朗泰生物科技有限公司
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However, the inventors only used UVB irradiation and fibroblast conditioned medium to pretreat the cells during the culture process of adipose-derived mesenchymal stem cells. The dose requirements are fine, which may increase the risk of cell contamination and cell damage; on the other hand, the pretreatment of the above methods does not specifically induce human adipose-derived mesenchymal stem cells to produce cytokines and growth factors that promote or repair hair follicle regeneration
[0007] Through the patent search, there is currently no clinically applicable patent that specifically induces human adipose-derived mesenchymal stem cells to produce hair growth-promoting cytokines and growth factors
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[0046] 1) Primary extraction and subculture of human adipose-derived mesenchymal stem cells:
[0047] 1.1) Primary extraction of human adipose-derived mesenchymal stem cells: wash the extracted fat particles 3 times with PBS, add 0.15% type Ⅰ collagenase, digest in a shaker at 37°C, 220r / min, 30min, and filter through a sterile filter Afterwards, take the filtered liquid and centrifuge it in a centrifuge at 800r / min for 5min, wash it twice with PBS, resuspend and inoculate it in serum-free medium MSC Xeno-Free SFM (CorningCat.No 88-600-CV), and inoculate it in an incubator Routine cultivation;
[0048] 1.2) Subculture of mesenchymal stem cells: when the cell fusion rate reaches 80%, subculture at a ratio of 1:3, and conventional subculture in an incubator.
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The invention relates to a preparation method of stem cell hair growth promoting factors capable of protecting and inducing regeneration of hair follicles. The method comprises the following steps of(1) performing primary extraction and subculturing on human adipose tissue-derived mesenchymal stem cells; (2) performing amplifying pretreatment on the human adipose tissue-derived mesenchymal stem cells; (3) performing specific induction pretreatment on the human adipose tissue-derived mesenchymal stem cells; and (4) performing collection and concentration on paracrine cytokine and growth factors of the human adipose tissue-derived mesenchymal stem cells. According to the preparation method disclosed by the invention, through specific stimulation and pretreatment on the human adipose tissue-derived mesenchymal stem cells in the direction of regeneration of the hair follicles, massive cytokine and growth factors capable of protecting and promoting hair growth, namely stem cell hair growthpromoting factors are secreted, and the stem cell hair growth promoting factors can be applied to hair loss prevention and hair growth promoting treatment of AGA.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of a stem cell growth factor for protecting and inducing hair follicle regeneration. Background technique [0002] Androgenetic alopecia (androgenetic alopecia.AGA), also known as seborrheic alopecia, is the main type of pathological alopecia, and it is also the most common type of hair loss clinically. The main manifestations of AGA are progressive hair thinning and hair follicle atrophy, and may be accompanied by greasy scalp, itching and excessive dandruff. Epidemiological data show that the total incidence of AGA in males in my country is about 20%, the prevalence of males aged 18 to 30 is about 3%, and the prevalence of males over 50 years old exceeds 35%. With the acceleration of the pace of life and the increase of pressure, the prevalence of AGA is increasing year by year, and the age of onset is gradually becoming younger. B...
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IPC IPC(8): A61K35/28A61P17/14C12N5/0775C12N5/077
CPCA61K35/28A61P17/14C12N5/0653C12N5/0667C12N2500/38C12N2500/40C12N2501/33C12N2501/39C12N2501/999
Inventor 胡储涵
Owner 陕西朗泰生物科技有限公司
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