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Chicken IgY bifunctional antibody for treating helicobacter pylori

A Helicobacter pylori and antibody technology, applied in the direction of antibodies, antibacterial drugs, anti-tumor drugs, etc., can solve the problems of long oral IgY antibody treatment time, difficult to cure, shorten treatment time, etc.

Inactive Publication Date: 2020-06-05
W H P M BIORES & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] The purpose of the patent of the present invention is to solve the problem of the drug resistance of Helicobacter pylori treatment drugs and the long treatment time of oral IgY antibody, which is not easy to be cured. The method of bifunctional antibody is used to modify The chicken IgY antibody against Helicobacter pylori acts as an antibiotic and anti-Helicobacter pylori antibody, and has the effect of dual-function treatment of Helicobacter pylori, shortens the treatment time, reduces the resistance of Helicobacter pylori to antibiotics, reduces the toxic and side effects of antibiotics, and optimizes Treatment options for Helicobacter pylori

Method used

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  • Chicken IgY bifunctional antibody for treating helicobacter pylori
  • Chicken IgY bifunctional antibody for treating helicobacter pylori
  • Chicken IgY bifunctional antibody for treating helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0034] Anti-Helicobacter pylori IgY antibody conjugated with metronidazole

[0035] plan 1

[0036]

[0037] Synthesis of metronidazole hemisuccinate (MNZ-SA): Add 0.85 g of metronidazole, 1.25 g of succinic anhydride, and 5 mL of anhydrous pyridine into a 10 mL reaction flask, stir evenly on a magnetic stirrer, then heat and stir at 100 ° C for 16 After 2 hours, pyridine was evaporated under reduced pressure, 5 mL of ice water was added, extracted with chloroform pre-cooled at 4°C, chloroform was evaporated under reduced pressure, evaporated to dryness at room temperature, the obtained crystals were redissolved in pre-cooled chloroform, and washed with ice water for 2 Next, dehydrated with anhydrous sodium sulfate, evaporated under reduced pressure to remove chloroform, evaporated to dryness at room temperature, and obtained needle-like white crystals, namely metronidazole hemisuccinate (MNZ-SA).

[0038] Dissolve 10.8 mg of MNZ-SA in 0.5 mL of N, N'-dimethylformamide, ad...

Embodiment example 2

[0047] Anti-Helicobacter pylori IgY antibody conjugated clarithromycin

[0048] plan 1

[0049]

[0050] 1) Dissolve 0.3 g of clarithromycin and 0.2 g of tert-butyl bromoacetate in 10 mL of acetone, reflux for 20 hours and then filter to obtain a white solid; dissolve the obtained solid in 5 mL of tetrahydrofuran and 5 mL of formic acid for 20 hours at room temperature, and evaporate the solvent to obtain The product is clarithromycin carboxyl modification.

[0051] 2) Accurately weigh 2.88 mg of NHS, 10.23 mg of EDC, and 1.8 mg of clarithromycin carboxyl modification, and add them to 230 microliters of DMF. This solution is called solution A. Solution A was incubated at room temperature for 24 hours with stirring in the dark.

[0052] 3) Accurately weigh 20 mg of IgY, and add 1.72 mL of 0.01 moL / L (pH=7.4) phosphate buffer saline (PBS). This solution is called solution B. Add the incubated solution A to solution B drop by drop, stir while adding, and stir at room temp...

Embodiment example 3

[0059] Anti-Helicobacter pylori IgY antibody conjugated norfloxacin

[0060] plan 1

[0061]

[0062] 1) Accurately weigh 2.88mg of NHS, 10.23mg of EDC, 3.86mg of norfloxacin (NFLX), and add them to 230 microliters of DMF. This solution is called solution A. Solution A was incubated at room temperature for 24 hours with stirring in the dark.

[0063] 2) Accurately weigh 26.4 mg of IgY, and add 1.72 mL of 0.01 moL / L (pH=7.4) phosphate buffer saline (PBS). This solution is called solution B. Add the incubated solution A to solution B drop by drop, stir while adding, and stir at room temperature for 3 hours. After the reaction is completed, put it into a dialysis bag, dialyze with 0.01moL / L PBS solution at 4°C for 3 days, and change the solution every day 3 times, the norfloxacin-IgY conjugate was obtained.

[0064] Scenario 2

[0065]

[0066] 1: Synthesis of norfloxacin derivatives

[0067] 1) Add norfloxacin (0.3194g, 1mmol) and DMF (10mL) into a 50mL round bottom...

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Abstract

The invention provides a chicken IgY bifunctional antibody for treating helicobacter pylori, relates to the field of therapeutic bifunctional antibodies and relates to a chicken IgY antibody for treating helicobacter pylori and a modification method of the chicken IgY antibody. The invention aims at adopting a method of a bifunctional antibody to solve the problems of drug resistance of helicobacter pylori treatment medicines, long treatment period and radical treatment difficulty of an orally-taking IgY antibody and achieves the effect of antibiotic and anti-helicobacter pylori antibody bifunctional helicobacter pylori treatment by modifying a helicobacter pylori resistant chicken IgY antibody with multiple chemical substances and multiple different methods, so that the treatment period can be shortened, the drug resistance of the helicobacter pylori on antibiotics can be reduced, toxic and side effects of antibiotics can be reduced, and the treatment scheme on the helicobacter pylorican be optimized.

Description

[0001] Technical field: the present invention relates to the field of therapeutic bifunctional antibodies, and relates to chicken IgY antibodies and modification methods thereof for anti-Helicobacter pylori treatment. Background technique: [0002] Since Australian scholars Warren and Marshall first successfully isolated Helicobacter pylori (Hp) from human gastric mucosa in 1983, many studies have shown that Hp infection has become a cause of human health that cannot be ignored. [0003] The infection of Helicobacter pylori is universal, and about 50% of the population in the world has this bacterial infection, and the proportion of developing countries with backward economy is even higher. The infection rate among adults in my country is about 70%, and its infection rate is similar to that of The incidence of gastric cancer is positively correlated, that is, in areas with high infection rates, the incidence of gastric cancer is also high. [0004] Helicobacter pylori infection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/40A61K47/60A61P31/04A61P1/04A61P35/00A61K31/4164A61K31/7048A61K31/496A61K31/43
CPCA61K39/40A61K31/4164A61K31/7048A61K31/496A61K31/43A61K47/60A61P31/04A61P1/04A61P35/00A61K2300/00
Inventor 万志强万积成马运国
Owner W H P M BIORES & TECH
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