Breeding method for low-abdominal-fat high-quality chickens
A fat, high-quality technology
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Embodiment 1
[0035] A breeding method for low-abdominal fat high-quality chickens, the breeding method infers the amount of deposited fat through the richness of the intestinal flora in the chicken feces, and uses the diversity of intestinal microorganisms as a selection index for predicting abdominal fat, Set up a basic chicken flock, eliminate and select breeder individual on the basis of the basic chicken flock, and described breeding method comprises the steps:
[0036] 1). Set up a basic chicken flock, and collect the feces in all basic chicken flocks to remove impurities in the feces, and the method for removing impurities in the feces is as follows: : the collected feces are put into the feces impurity pretreatment equipment, and the feces impurity pretreatment equipment includes a collecting tank, a filtering device and a stirring device, the filtering device is installed on the top of the collecting tank, and the stirring device is installed In the collecting tank, several vibrati...
Embodiment 2
[0045] In this embodiment, live experiments were adopted, and the test animals came from a certain original breeding farm, and Qingyuan Ma pheasant hens of the same age were used as the test subjects, and all test chickens adopted exactly the same diet structure and the same feeding conditions.
[0046] 1). The clean feces of all chickens in the basic group will be collected for microbial diversity analysis when they are raised to 120 days of age; the diversity analysis includes the following steps: primer design: using international general primer 515f / 806r, wherein, the Said 515f is: GTGCCAGCMGCCGCGGTAA, said 806r is: GGACTACHVGGGTWTCTAAT; DNA extraction: use microbial DNA extraction kit (Qiagen) to extract the total DNA of cecum contents; DNA amplification: use the above-mentioned 515f / 806r primer to amplify the 16S rDNA In the V4 region, the PCR product was purified and recovered with the QIAquick kit (QIAGEN); DNA sequencing: the PCR product was sequenced using Hiseq2500, ...
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