Specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae

A mycoplasma, specific technology, applied in the field of molecular biology, can solve the problems of reducing the clinical practicability of nucleic acid diagnostic methods to detect Mccp, and achieve the effect of low cost

Active Publication Date: 2020-06-05
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

With the continuous improvement of technology, these nucleic acid diagnostic methods have good specificity and sensitivity, but on the one hand, most of the amplification systems are based on DNA samples, which require professional laboratory equipment

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  • Specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae
  • Specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae
  • Specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae

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Embodiment Construction

[0020] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] 1. Reagents:

[0022] Enzyme (2×Flash PCR MasterMix (Dye), Kangwei Century), DNA extraction kit (bacterial genomic DNA extraction kit, Tiangen), and DNA samples were kept in the laboratory. Strains: All the strains used in the experiment were preserved by Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0023] 2. Mccp-specific sequence

[0024] By comparative genomics, the entire genome sequence of Mccp1601 and other members of the mycoplasma cluster (Mmc, Mmm, Mcc, Ml) were blasted. Some Mccp-specific sequences were obtained. According to the latest NCBI...

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Abstract

The invention discloses specific PCR primers used for detecting mycoplasma capricolum subsp. capripneumoniae. According to the specific PCR primers used for detecting the mycoplasma capricolum subsp.capripneumoniae, firstly, by conducting gene sequencing and comparing on the mycoplasma capricolum subsp. capripneumoniae, a target sequence used for detecting the mycoplasma capricolum subsp. capripneumoniae is screened out, a gene sequence of the target sequence is shown in SEQ ID NO.1 as shown in the description, then the specific PCR primers used for detecting the mycoplasma capricolum subsp.capripneumoniae are designed, the specific PCR primers used for detecting the mycoplasma capricolum subsp. capripneumoniae can distinguish the Mccp (mycoplasma capricolum subsp. capripneumoniae) fromother members of mycoplasma capricolum clusters and common ruminant mycoplasmas, show good specificity, and can be used as a novel diagnosis target, and on the basis of the specific PCR primers used for detecting the mycoplasma capricolum subsp. capripneumoniae, an Mccp diagnosis technology with higher clinical practicability is built.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a specific PCR primer for detecting mycoplasma goat pneumonia subspecies. Background technique [0002] Contagious caprine pleuropneumonia (CCPP) is a highly contagious goat respiratory disease caused by Mycoplasma capricolumsubsp. The rate is 60% to 80%, and it is one of the OIE legally notifiable diseases. The goat industry in more than 40 countries around the world is threatened by CCPP. Our country first reported goat "rotten lung disease" in Xinjiang in 1922, and it was widely popular in Northwest and North China. It is easy to control, but in recent years, the number of case reports has increased, showing a comeback trend, and the difficulty of control has increased. [0003] The CCPP pathogen Mccp belongs to the mycoplasma cluster. In addition to Mccp, members of this cluster include: Mycoides subsp.mycoides (Mmm), Mycoides subsp.capri (M.mycoides s...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/35
CPCC12Q1/689
Inventor 颜新敏吴娅琴储岳峰郝华芳陈胜利马丽娜刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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