A method for the detection of production-increasing amines by dispersive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry
A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of dispersive solid phase extraction combined with liquid chromatography tandem mass spectrometry to detect production-increasing amines, can solve the problems of large amount of organic reagents, and achieve high accuracy, high precision, and easy operation.
Active Publication Date: 2022-04-15
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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[0007] The purpose of the present invention is to provide a method for detecting the increased production of amines in combination with dispersive solid phase extraction combined with liquid chromatography tandem mass spectrometry, which is used to solve the problem of the use of organic reagents in existing traditional extraction techniques. volume problem
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[0019] This method of dispersive solid phase extraction combined with liquid chromatography tandem mass spectrometry to detect the increased production of amines uses PSA+GCB as the dispersed solid phase extraction adsorbent to purify the increased production of amines in the mung bean extract. After purifying the mung bean sample extract, UHPLC- MS / MS was used for qualitative and quantitative analysis. The pretreatment steps include weighing, vortex, centrifugation, and concentration steps; the instrumental analysis includes the qualitative and quantitative analysis of the target compound using UHPLC-MS / MS, as follows:
[0020] 1. Use PSA+GCB as the dispersive solid-phase extraction adsorbent to purify the amine production in the mung bean extract, specifically: weigh 2g mung bean sample into a 50mL polytetrafluoroethylene centrifuge tube, then add 0.2g sodium chloride, 1 g of anhydrous magnesium sulfate and 20 mL of acetic acid-acetonitrile solution with a volume ratio of 1:...
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The present invention relates to a method for detecting increased production of amines by means of dispersive solid-phase extraction combined with liquid chromatography tandem mass spectrometry, which comprises: weighing 2 g of mung bean samples into a 50 mL polytetrafluoroethylene centrifuge tube, and then adding 0.2 g of sodium chloride, 1 g of anhydrous Magnesium sulfate, 20 mL of acetic acid-acetonitrile solution with a volume ratio of 1:99, vortexed for 1 min, centrifuged at 10000 r / min for 3 min, took 5 mL of the supernatant and transferred it to a 15 mL PTFE centrifuge tube, added 50 mg of GCB, 200 mg PSA, mediated and shaken for 3 minutes, centrifuged at 10000 r / min for 5 minutes, and the supernatant was passed through a 0.22 μm microporous membrane; the qualitative and quantitative analysis of the mung bean extract was carried out by liquid chromatography tandem mass spectrometry, and the production increase in the mung bean extract was detected. amine. The invention has the characteristics of less solvent consumption, simple and fast operation, high accuracy and high recovery rate.
Description
1. Technical field: [0001] The invention relates to the technical field of residue detection in miscellaneous grains, in particular to a method for detecting production-increasing amines in combination with dispersive solid-phase extraction and liquid chromatography tandem mass spectrometry. 2. Background technology: [0002] Plant growth regulators can be used to regulate the synthesis of proteins, enzymes and nucleic acids in plants, and to regulate the different stages of plant growth and development, such as germination, rooting, flowering, fruiting, defoliation, dormancy and cell elongation, organ differentiation, flower buds, etc. Differentiation, etc. play a regulatory and control role. Some of them can increase the content of vitamins and chlorophyll in plants, some can change their plant type, some can enhance the ability of plants to resist drought, cold, salinity and pests, and some can increase production. Field crops, commercial crops, forest trees, vegetables,...
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IPC IPC(8): G01N30/02G01N30/08G01N30/14G01N30/34G01N30/32G01N30/54
CPCG01N30/02G01N30/08G01N30/14G01N30/34G01N30/32G01N30/54G01N2030/324
Inventor 高玉玲郭永霞李伟庆李东雪闫岩闫睿
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY